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Isolation of potent SARS-CoV-2 neutralizing antibodies and protection from disease in a small animal model
Countermeasures to prevent and treat coronavirus disease 2019 (COVID-19) are a global health priority. We enrolled a cohort of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)–recovered participants, developed neutralization assays to investigate antibody responses, adapted our high-thro...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7299280/ https://www.ncbi.nlm.nih.gov/pubmed/32540903 http://dx.doi.org/10.1126/science.abc7520 |
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author | Rogers, Thomas F. Zhao, Fangzhu Huang, Deli Beutler, Nathan Burns, Alison He, Wan-ting Limbo, Oliver Smith, Chloe Song, Ge Woehl, Jordan Yang, Linlin Abbott, Robert K. Callaghan, Sean Garcia, Elijah Hurtado, Jonathan Parren, Mara Peng, Linghang Ramirez, Sydney Ricketts, James Ricciardi, Michael J. Rawlings, Stephen A. Wu, Nicholas C. Yuan, Meng Smith, Davey M. Nemazee, David Teijaro, John R. Voss, James E. Wilson, Ian A. Andrabi, Raiees Briney, Bryan Landais, Elise Sok, Devin Jardine, Joseph G. Burton, Dennis R. |
author_facet | Rogers, Thomas F. Zhao, Fangzhu Huang, Deli Beutler, Nathan Burns, Alison He, Wan-ting Limbo, Oliver Smith, Chloe Song, Ge Woehl, Jordan Yang, Linlin Abbott, Robert K. Callaghan, Sean Garcia, Elijah Hurtado, Jonathan Parren, Mara Peng, Linghang Ramirez, Sydney Ricketts, James Ricciardi, Michael J. Rawlings, Stephen A. Wu, Nicholas C. Yuan, Meng Smith, Davey M. Nemazee, David Teijaro, John R. Voss, James E. Wilson, Ian A. Andrabi, Raiees Briney, Bryan Landais, Elise Sok, Devin Jardine, Joseph G. Burton, Dennis R. |
author_sort | Rogers, Thomas F. |
collection | PubMed |
description | Countermeasures to prevent and treat coronavirus disease 2019 (COVID-19) are a global health priority. We enrolled a cohort of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)–recovered participants, developed neutralization assays to investigate antibody responses, adapted our high-throughput antibody generation pipeline to rapidly screen more than 1800 antibodies, and established an animal model to test protection. We isolated potent neutralizing antibodies (nAbs) to two epitopes on the receptor binding domain (RBD) and to distinct non-RBD epitopes on the spike (S) protein. As indicated by maintained weight and low lung viral titers in treated animals, the passive transfer of a nAb provides protection against disease in high-dose SARS-CoV-2 challenge in Syrian hamsters. The study suggests a role for nAbs in prophylaxis, and potentially therapy, of COVID-19. The nAbs also define protective epitopes to guide vaccine design. |
format | Online Article Text |
id | pubmed-7299280 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-72992802020-06-29 Isolation of potent SARS-CoV-2 neutralizing antibodies and protection from disease in a small animal model Rogers, Thomas F. Zhao, Fangzhu Huang, Deli Beutler, Nathan Burns, Alison He, Wan-ting Limbo, Oliver Smith, Chloe Song, Ge Woehl, Jordan Yang, Linlin Abbott, Robert K. Callaghan, Sean Garcia, Elijah Hurtado, Jonathan Parren, Mara Peng, Linghang Ramirez, Sydney Ricketts, James Ricciardi, Michael J. Rawlings, Stephen A. Wu, Nicholas C. Yuan, Meng Smith, Davey M. Nemazee, David Teijaro, John R. Voss, James E. Wilson, Ian A. Andrabi, Raiees Briney, Bryan Landais, Elise Sok, Devin Jardine, Joseph G. Burton, Dennis R. Science Research Articles Countermeasures to prevent and treat coronavirus disease 2019 (COVID-19) are a global health priority. We enrolled a cohort of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)–recovered participants, developed neutralization assays to investigate antibody responses, adapted our high-throughput antibody generation pipeline to rapidly screen more than 1800 antibodies, and established an animal model to test protection. We isolated potent neutralizing antibodies (nAbs) to two epitopes on the receptor binding domain (RBD) and to distinct non-RBD epitopes on the spike (S) protein. As indicated by maintained weight and low lung viral titers in treated animals, the passive transfer of a nAb provides protection against disease in high-dose SARS-CoV-2 challenge in Syrian hamsters. The study suggests a role for nAbs in prophylaxis, and potentially therapy, of COVID-19. The nAbs also define protective epitopes to guide vaccine design. American Association for the Advancement of Science 2020-08-21 2020-06-15 /pmc/articles/PMC7299280/ /pubmed/32540903 http://dx.doi.org/10.1126/science.abc7520 Text en Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Rogers, Thomas F. Zhao, Fangzhu Huang, Deli Beutler, Nathan Burns, Alison He, Wan-ting Limbo, Oliver Smith, Chloe Song, Ge Woehl, Jordan Yang, Linlin Abbott, Robert K. Callaghan, Sean Garcia, Elijah Hurtado, Jonathan Parren, Mara Peng, Linghang Ramirez, Sydney Ricketts, James Ricciardi, Michael J. Rawlings, Stephen A. Wu, Nicholas C. Yuan, Meng Smith, Davey M. Nemazee, David Teijaro, John R. Voss, James E. Wilson, Ian A. Andrabi, Raiees Briney, Bryan Landais, Elise Sok, Devin Jardine, Joseph G. Burton, Dennis R. Isolation of potent SARS-CoV-2 neutralizing antibodies and protection from disease in a small animal model |
title | Isolation of potent SARS-CoV-2 neutralizing antibodies and protection from disease in a small animal model |
title_full | Isolation of potent SARS-CoV-2 neutralizing antibodies and protection from disease in a small animal model |
title_fullStr | Isolation of potent SARS-CoV-2 neutralizing antibodies and protection from disease in a small animal model |
title_full_unstemmed | Isolation of potent SARS-CoV-2 neutralizing antibodies and protection from disease in a small animal model |
title_short | Isolation of potent SARS-CoV-2 neutralizing antibodies and protection from disease in a small animal model |
title_sort | isolation of potent sars-cov-2 neutralizing antibodies and protection from disease in a small animal model |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7299280/ https://www.ncbi.nlm.nih.gov/pubmed/32540903 http://dx.doi.org/10.1126/science.abc7520 |
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