Guide-free Cas9 from pathogenic Campylobacter jejuni bacteria causes severe damage to DNA

CRISPR-Cas9 systems are enriched in human pathogenic bacteria and have been linked to cytotoxicity by an unknown mechanism. Here, we show that upon infection of human cells, Campylobacter jejuni secretes its Cas9 (CjeCas9) nuclease into their cytoplasm. Next, a native nuclear localization signal ena...

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Detalles Bibliográficos
Autores principales: Saha, Chinmoy, Mohanraju, Prarthana, Stubbs, Andrew, Dugar, Gaurav, Hoogstrate, Youri, Kremers, Gert-Jan, van Cappellen, Wiggert A., Horst-Kreft, Deborah, Laffeber, Charlie, Lebbink, Joyce H.G., Bruens, Serena, Gaskin, Duncan, Beerens, Dior, Klunder, Maarten, Joosten, Rob, Demmers, Jeroen A. A., van Gent, Dik, Mouton, Johan W., van der Spek, Peter J., van der Oost, John, van Baarlen, Peter, Louwen, Rogier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2020
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7299616/
https://www.ncbi.nlm.nih.gov/pubmed/32596446
http://dx.doi.org/10.1126/sciadv.aaz4849

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