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Supracellular Actomyosin Mediates Cell-Cell Communication and Shapes Collective Migratory Morphology

During collective cell migration, front cells tend to extend a predominant leading protrusion, which is rarely present in cells at the side or rear positions. Using Drosophila border cells (BCs) as a model system of collective migration, we revealed the presence of a supracellular actomyosin network...

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Detalles Bibliográficos
Autores principales: Wang, Heng, Guo, Xuan, Wang, Xianping, Wang, Xiaobo, Chen, Jiong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7300135/
https://www.ncbi.nlm.nih.gov/pubmed/32535019
http://dx.doi.org/10.1016/j.isci.2020.101204
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author Wang, Heng
Guo, Xuan
Wang, Xianping
Wang, Xiaobo
Chen, Jiong
author_facet Wang, Heng
Guo, Xuan
Wang, Xianping
Wang, Xiaobo
Chen, Jiong
author_sort Wang, Heng
collection PubMed
description During collective cell migration, front cells tend to extend a predominant leading protrusion, which is rarely present in cells at the side or rear positions. Using Drosophila border cells (BCs) as a model system of collective migration, we revealed the presence of a supracellular actomyosin network at the peripheral surface of BC clusters. We demonstrated that the Myosin II-mediated mechanical tension as exerted by this peripheral supracellular network not only mediated cell-cell communication between leading BC and non-leading BCs but also restrained formation of prominent protrusions at non-leading BCs. Further analysis revealed that a cytoplasmic dendritic actin network that depends on the function of Arp2/3 complex interacted with the actomyosin network. Together, our data suggest that the outward pushing or protrusive force as generated from Arp2/3-dependent actin polymerization and the inward restraining force as produced from the supracellular actomyosin network together determine the collective and polarized morphology of migratory BCs.
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spelling pubmed-73001352020-06-22 Supracellular Actomyosin Mediates Cell-Cell Communication and Shapes Collective Migratory Morphology Wang, Heng Guo, Xuan Wang, Xianping Wang, Xiaobo Chen, Jiong iScience Article During collective cell migration, front cells tend to extend a predominant leading protrusion, which is rarely present in cells at the side or rear positions. Using Drosophila border cells (BCs) as a model system of collective migration, we revealed the presence of a supracellular actomyosin network at the peripheral surface of BC clusters. We demonstrated that the Myosin II-mediated mechanical tension as exerted by this peripheral supracellular network not only mediated cell-cell communication between leading BC and non-leading BCs but also restrained formation of prominent protrusions at non-leading BCs. Further analysis revealed that a cytoplasmic dendritic actin network that depends on the function of Arp2/3 complex interacted with the actomyosin network. Together, our data suggest that the outward pushing or protrusive force as generated from Arp2/3-dependent actin polymerization and the inward restraining force as produced from the supracellular actomyosin network together determine the collective and polarized morphology of migratory BCs. Elsevier 2020-05-27 /pmc/articles/PMC7300135/ /pubmed/32535019 http://dx.doi.org/10.1016/j.isci.2020.101204 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Wang, Heng
Guo, Xuan
Wang, Xianping
Wang, Xiaobo
Chen, Jiong
Supracellular Actomyosin Mediates Cell-Cell Communication and Shapes Collective Migratory Morphology
title Supracellular Actomyosin Mediates Cell-Cell Communication and Shapes Collective Migratory Morphology
title_full Supracellular Actomyosin Mediates Cell-Cell Communication and Shapes Collective Migratory Morphology
title_fullStr Supracellular Actomyosin Mediates Cell-Cell Communication and Shapes Collective Migratory Morphology
title_full_unstemmed Supracellular Actomyosin Mediates Cell-Cell Communication and Shapes Collective Migratory Morphology
title_short Supracellular Actomyosin Mediates Cell-Cell Communication and Shapes Collective Migratory Morphology
title_sort supracellular actomyosin mediates cell-cell communication and shapes collective migratory morphology
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7300135/
https://www.ncbi.nlm.nih.gov/pubmed/32535019
http://dx.doi.org/10.1016/j.isci.2020.101204
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