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Analysis of Yarrowia lipolytica growth, catabolism, and terpenoid biosynthesis during utilization of lipid-derived feedstock

This study employs biomass growth analyses and (13)C-isotope tracing to investigate lipid feedstock utilization by Yarrowia lipolytica. Compared to glucose, oil-feedstock in the minimal medium increases the yeast's biomass yields and cell sizes, but decreases its protein content (<20% of tot...

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Autores principales: Worland, Alyssa M., Czajka, Jeffrey J., Xing, Yun, Harper, Willie F., Moore, Aryiana, Xiao, Zhengyang, Han, Zhenlin, Wang, Yechun, Su, Wei Wen, Tang, Yinjie J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7300164/
https://www.ncbi.nlm.nih.gov/pubmed/32577396
http://dx.doi.org/10.1016/j.mec.2020.e00130
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author Worland, Alyssa M.
Czajka, Jeffrey J.
Xing, Yun
Harper, Willie F.
Moore, Aryiana
Xiao, Zhengyang
Han, Zhenlin
Wang, Yechun
Su, Wei Wen
Tang, Yinjie J.
author_facet Worland, Alyssa M.
Czajka, Jeffrey J.
Xing, Yun
Harper, Willie F.
Moore, Aryiana
Xiao, Zhengyang
Han, Zhenlin
Wang, Yechun
Su, Wei Wen
Tang, Yinjie J.
author_sort Worland, Alyssa M.
collection PubMed
description This study employs biomass growth analyses and (13)C-isotope tracing to investigate lipid feedstock utilization by Yarrowia lipolytica. Compared to glucose, oil-feedstock in the minimal medium increases the yeast's biomass yields and cell sizes, but decreases its protein content (<20% of total biomass) and enzyme abundances for product synthesis. Labeling results indicate a segregated metabolic network (the glycolysis vs. the TCA cycle) during co-catabolism of sugars (glucose or glycerol) with fatty acid substrates, which facilitates resource allocations for biosynthesis without catabolite repressions. This study has also examined the performance of a β-carotene producing strain in different growth mediums. Canola oil-containing yeast-peptone (YP) has resulted in the best β-carotene titer (121 ± 13 mg/L), two-fold higher than the glucose based YP medium. These results highlight the potential of Y. lipolytica for the valorization of waste-derived lipid feedstock.
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spelling pubmed-73001642020-06-22 Analysis of Yarrowia lipolytica growth, catabolism, and terpenoid biosynthesis during utilization of lipid-derived feedstock Worland, Alyssa M. Czajka, Jeffrey J. Xing, Yun Harper, Willie F. Moore, Aryiana Xiao, Zhengyang Han, Zhenlin Wang, Yechun Su, Wei Wen Tang, Yinjie J. Metab Eng Commun Full Length Article This study employs biomass growth analyses and (13)C-isotope tracing to investigate lipid feedstock utilization by Yarrowia lipolytica. Compared to glucose, oil-feedstock in the minimal medium increases the yeast's biomass yields and cell sizes, but decreases its protein content (<20% of total biomass) and enzyme abundances for product synthesis. Labeling results indicate a segregated metabolic network (the glycolysis vs. the TCA cycle) during co-catabolism of sugars (glucose or glycerol) with fatty acid substrates, which facilitates resource allocations for biosynthesis without catabolite repressions. This study has also examined the performance of a β-carotene producing strain in different growth mediums. Canola oil-containing yeast-peptone (YP) has resulted in the best β-carotene titer (121 ± 13 mg/L), two-fold higher than the glucose based YP medium. These results highlight the potential of Y. lipolytica for the valorization of waste-derived lipid feedstock. Elsevier 2020-05-16 /pmc/articles/PMC7300164/ /pubmed/32577396 http://dx.doi.org/10.1016/j.mec.2020.e00130 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Full Length Article
Worland, Alyssa M.
Czajka, Jeffrey J.
Xing, Yun
Harper, Willie F.
Moore, Aryiana
Xiao, Zhengyang
Han, Zhenlin
Wang, Yechun
Su, Wei Wen
Tang, Yinjie J.
Analysis of Yarrowia lipolytica growth, catabolism, and terpenoid biosynthesis during utilization of lipid-derived feedstock
title Analysis of Yarrowia lipolytica growth, catabolism, and terpenoid biosynthesis during utilization of lipid-derived feedstock
title_full Analysis of Yarrowia lipolytica growth, catabolism, and terpenoid biosynthesis during utilization of lipid-derived feedstock
title_fullStr Analysis of Yarrowia lipolytica growth, catabolism, and terpenoid biosynthesis during utilization of lipid-derived feedstock
title_full_unstemmed Analysis of Yarrowia lipolytica growth, catabolism, and terpenoid biosynthesis during utilization of lipid-derived feedstock
title_short Analysis of Yarrowia lipolytica growth, catabolism, and terpenoid biosynthesis during utilization of lipid-derived feedstock
title_sort analysis of yarrowia lipolytica growth, catabolism, and terpenoid biosynthesis during utilization of lipid-derived feedstock
topic Full Length Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7300164/
https://www.ncbi.nlm.nih.gov/pubmed/32577396
http://dx.doi.org/10.1016/j.mec.2020.e00130
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