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Analysis of Yarrowia lipolytica growth, catabolism, and terpenoid biosynthesis during utilization of lipid-derived feedstock
This study employs biomass growth analyses and (13)C-isotope tracing to investigate lipid feedstock utilization by Yarrowia lipolytica. Compared to glucose, oil-feedstock in the minimal medium increases the yeast's biomass yields and cell sizes, but decreases its protein content (<20% of tot...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7300164/ https://www.ncbi.nlm.nih.gov/pubmed/32577396 http://dx.doi.org/10.1016/j.mec.2020.e00130 |
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author | Worland, Alyssa M. Czajka, Jeffrey J. Xing, Yun Harper, Willie F. Moore, Aryiana Xiao, Zhengyang Han, Zhenlin Wang, Yechun Su, Wei Wen Tang, Yinjie J. |
author_facet | Worland, Alyssa M. Czajka, Jeffrey J. Xing, Yun Harper, Willie F. Moore, Aryiana Xiao, Zhengyang Han, Zhenlin Wang, Yechun Su, Wei Wen Tang, Yinjie J. |
author_sort | Worland, Alyssa M. |
collection | PubMed |
description | This study employs biomass growth analyses and (13)C-isotope tracing to investigate lipid feedstock utilization by Yarrowia lipolytica. Compared to glucose, oil-feedstock in the minimal medium increases the yeast's biomass yields and cell sizes, but decreases its protein content (<20% of total biomass) and enzyme abundances for product synthesis. Labeling results indicate a segregated metabolic network (the glycolysis vs. the TCA cycle) during co-catabolism of sugars (glucose or glycerol) with fatty acid substrates, which facilitates resource allocations for biosynthesis without catabolite repressions. This study has also examined the performance of a β-carotene producing strain in different growth mediums. Canola oil-containing yeast-peptone (YP) has resulted in the best β-carotene titer (121 ± 13 mg/L), two-fold higher than the glucose based YP medium. These results highlight the potential of Y. lipolytica for the valorization of waste-derived lipid feedstock. |
format | Online Article Text |
id | pubmed-7300164 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-73001642020-06-22 Analysis of Yarrowia lipolytica growth, catabolism, and terpenoid biosynthesis during utilization of lipid-derived feedstock Worland, Alyssa M. Czajka, Jeffrey J. Xing, Yun Harper, Willie F. Moore, Aryiana Xiao, Zhengyang Han, Zhenlin Wang, Yechun Su, Wei Wen Tang, Yinjie J. Metab Eng Commun Full Length Article This study employs biomass growth analyses and (13)C-isotope tracing to investigate lipid feedstock utilization by Yarrowia lipolytica. Compared to glucose, oil-feedstock in the minimal medium increases the yeast's biomass yields and cell sizes, but decreases its protein content (<20% of total biomass) and enzyme abundances for product synthesis. Labeling results indicate a segregated metabolic network (the glycolysis vs. the TCA cycle) during co-catabolism of sugars (glucose or glycerol) with fatty acid substrates, which facilitates resource allocations for biosynthesis without catabolite repressions. This study has also examined the performance of a β-carotene producing strain in different growth mediums. Canola oil-containing yeast-peptone (YP) has resulted in the best β-carotene titer (121 ± 13 mg/L), two-fold higher than the glucose based YP medium. These results highlight the potential of Y. lipolytica for the valorization of waste-derived lipid feedstock. Elsevier 2020-05-16 /pmc/articles/PMC7300164/ /pubmed/32577396 http://dx.doi.org/10.1016/j.mec.2020.e00130 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Full Length Article Worland, Alyssa M. Czajka, Jeffrey J. Xing, Yun Harper, Willie F. Moore, Aryiana Xiao, Zhengyang Han, Zhenlin Wang, Yechun Su, Wei Wen Tang, Yinjie J. Analysis of Yarrowia lipolytica growth, catabolism, and terpenoid biosynthesis during utilization of lipid-derived feedstock |
title | Analysis of Yarrowia lipolytica growth, catabolism, and terpenoid biosynthesis during utilization of lipid-derived feedstock |
title_full | Analysis of Yarrowia lipolytica growth, catabolism, and terpenoid biosynthesis during utilization of lipid-derived feedstock |
title_fullStr | Analysis of Yarrowia lipolytica growth, catabolism, and terpenoid biosynthesis during utilization of lipid-derived feedstock |
title_full_unstemmed | Analysis of Yarrowia lipolytica growth, catabolism, and terpenoid biosynthesis during utilization of lipid-derived feedstock |
title_short | Analysis of Yarrowia lipolytica growth, catabolism, and terpenoid biosynthesis during utilization of lipid-derived feedstock |
title_sort | analysis of yarrowia lipolytica growth, catabolism, and terpenoid biosynthesis during utilization of lipid-derived feedstock |
topic | Full Length Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7300164/ https://www.ncbi.nlm.nih.gov/pubmed/32577396 http://dx.doi.org/10.1016/j.mec.2020.e00130 |
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