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Development of ultrahigh‐performance liquid chromatography/mass spectrometry and ultrahigh‐performance supercritical fluid chromatography/mass spectrometry assays to determine the concentration of Bitrex™ and sodium saccharin in homemade facemask fit testing solutions

Fast and easily transferable chromatography/mass spectrometry assays were required to detect and quantify the amount of Bitrex™ and sodium saccharin in homemade facemask fit testing solutions. METHODS: Bitrex™ solutions were analysed using reversed‐phase ultrahigh‐performance liquid chromatography c...

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Detalles Bibliográficos
Autores principales: Herniman, Julie M., Langley, G. John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7300544/
https://www.ncbi.nlm.nih.gov/pubmed/32492215
http://dx.doi.org/10.1002/rcm.8848
Descripción
Sumario:Fast and easily transferable chromatography/mass spectrometry assays were required to detect and quantify the amount of Bitrex™ and sodium saccharin in homemade facemask fit testing solutions. METHODS: Bitrex™ solutions were analysed using reversed‐phase ultrahigh‐performance liquid chromatography coupled with positive ion electrospray ionisation mass spectrometry (UHPLC/ESI‐MS). Separation was achieved using a mobile phase gradient with an Acquity BEH C18‐packed column. Sodium saccharin solutions were analysed using ultrahigh‐performance supercritical fluid chromatography coupled with negative ion electrospray ionisation (UHPSFC/ESI‐MS). Separation was achieved using isocratic elution with an Acquity UPC(2) Torus Diol packed column and a methanol (25 mM ammonium acetate) co‐solvent. RESULTS: The calibration curves obtained using the ratio of the active compound to an internal standard generated linear regression values (R(2)) >0.99. Samples analysed prior to and after an autoclave sterilisation process and bottling gave repeatable measurements within 10% of the expected concentration. CONCLUSIONS: The two assays afford a fast robust and quantitative analytical method for the detection of the active components used to test the efficacy of the homemade facemask testing solutions.