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Indoor dust extracellular vesicles promote cancer lung metastasis by inducing tumour necrosis factor-α

Indoor pollutants are important problems to public health. Among indoor pollutants, indoor dust contains extracellular vesicles (EVs), which are associated with pulmonary inflammation. However, it has not been reported whether indoor dust EVs affect the cancer lung metastasis. In this study, we isol...

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Autores principales: Dinh, Nhung Thi Hong, Lee, Jaewook, Lee, Jaemin, Kim, Sang Soo, Go, Gyeongyun, Bae, Seoyoon, Jun, Ye In, Yoon, Yae Jin, Roh, Tae-Young, Gho, Yong Song
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7301719/
https://www.ncbi.nlm.nih.gov/pubmed/32595916
http://dx.doi.org/10.1080/20013078.2020.1766821
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author Dinh, Nhung Thi Hong
Lee, Jaewook
Lee, Jaemin
Kim, Sang Soo
Go, Gyeongyun
Bae, Seoyoon
Jun, Ye In
Yoon, Yae Jin
Roh, Tae-Young
Gho, Yong Song
author_facet Dinh, Nhung Thi Hong
Lee, Jaewook
Lee, Jaemin
Kim, Sang Soo
Go, Gyeongyun
Bae, Seoyoon
Jun, Ye In
Yoon, Yae Jin
Roh, Tae-Young
Gho, Yong Song
author_sort Dinh, Nhung Thi Hong
collection PubMed
description Indoor pollutants are important problems to public health. Among indoor pollutants, indoor dust contains extracellular vesicles (EVs), which are associated with pulmonary inflammation. However, it has not been reported whether indoor dust EVs affect the cancer lung metastasis. In this study, we isolated indoor dust EVs and investigated their roles in cancer lung metastasis. Upon intranasal administration, indoor dust EVs enhanced mouse melanoma lung metastasis in a dose-dependent manner in mice. Pre-treatment or co-treatment of indoor dust EVs significantly promoted melanoma lung metastasis, whereas post-treatment of the EVs did not. In addition, the lung lysates from indoor dust EV-treated mice significantly increased tumour cell migration in vitro. We observed that tumour necrosis factor-α played important roles in indoor dust EV-mediated promotion of tumour cell migration in vitro and cancer lung metastasis in vivo. Furthermore, Pseudomonas EVs, the main components of indoor dust EVs, and indoor dust EVs showed comparable effects in promoting tumour cell migration in vitro and cancer lung metastasis in vivo. Taken together, our results suggest that indoor dust EVs, at least partly contributed by Pseudomonas EVs, are potential promoting agents of cancer lung metastasis.
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spelling pubmed-73017192020-06-25 Indoor dust extracellular vesicles promote cancer lung metastasis by inducing tumour necrosis factor-α Dinh, Nhung Thi Hong Lee, Jaewook Lee, Jaemin Kim, Sang Soo Go, Gyeongyun Bae, Seoyoon Jun, Ye In Yoon, Yae Jin Roh, Tae-Young Gho, Yong Song J Extracell Vesicles Research Article Indoor pollutants are important problems to public health. Among indoor pollutants, indoor dust contains extracellular vesicles (EVs), which are associated with pulmonary inflammation. However, it has not been reported whether indoor dust EVs affect the cancer lung metastasis. In this study, we isolated indoor dust EVs and investigated their roles in cancer lung metastasis. Upon intranasal administration, indoor dust EVs enhanced mouse melanoma lung metastasis in a dose-dependent manner in mice. Pre-treatment or co-treatment of indoor dust EVs significantly promoted melanoma lung metastasis, whereas post-treatment of the EVs did not. In addition, the lung lysates from indoor dust EV-treated mice significantly increased tumour cell migration in vitro. We observed that tumour necrosis factor-α played important roles in indoor dust EV-mediated promotion of tumour cell migration in vitro and cancer lung metastasis in vivo. Furthermore, Pseudomonas EVs, the main components of indoor dust EVs, and indoor dust EVs showed comparable effects in promoting tumour cell migration in vitro and cancer lung metastasis in vivo. Taken together, our results suggest that indoor dust EVs, at least partly contributed by Pseudomonas EVs, are potential promoting agents of cancer lung metastasis. Taylor & Francis 2020-05-19 /pmc/articles/PMC7301719/ /pubmed/32595916 http://dx.doi.org/10.1080/20013078.2020.1766821 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles. http://creativecommons.org/licenses/by-nc/4.0/ http://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Dinh, Nhung Thi Hong
Lee, Jaewook
Lee, Jaemin
Kim, Sang Soo
Go, Gyeongyun
Bae, Seoyoon
Jun, Ye In
Yoon, Yae Jin
Roh, Tae-Young
Gho, Yong Song
Indoor dust extracellular vesicles promote cancer lung metastasis by inducing tumour necrosis factor-α
title Indoor dust extracellular vesicles promote cancer lung metastasis by inducing tumour necrosis factor-α
title_full Indoor dust extracellular vesicles promote cancer lung metastasis by inducing tumour necrosis factor-α
title_fullStr Indoor dust extracellular vesicles promote cancer lung metastasis by inducing tumour necrosis factor-α
title_full_unstemmed Indoor dust extracellular vesicles promote cancer lung metastasis by inducing tumour necrosis factor-α
title_short Indoor dust extracellular vesicles promote cancer lung metastasis by inducing tumour necrosis factor-α
title_sort indoor dust extracellular vesicles promote cancer lung metastasis by inducing tumour necrosis factor-α
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7301719/
https://www.ncbi.nlm.nih.gov/pubmed/32595916
http://dx.doi.org/10.1080/20013078.2020.1766821
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