Cargando…

Early Detection of Non-Small Cell Lung Cancer by Using a 12-microRNA Panel and a Nomogram for Assistant Diagnosis

Background: We previously identified a 12-microRNA (miRNA) panel (miRNA-17, miRNA-146a, miRNA-200b, miRNA-182, miRNA-155, miRNA-221, miRNA-205, miRNA-126, miRNA-7, miRNA-21, miRNA-145, and miRNA-210) that aided in the early diagnosis of non-small cell lung cancer (NSCLC). We validated the diagnostic...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Weidong, Chen, Dongni, Chen, Weiwei, Xin, Ziya, Huang, Zirui, Zhang, Xuewen, Xi, Kexing, Wang, Gongming, Zhang, Rusi, Zhao, Dechang, Liu, Li, Zhang, Lanjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7301755/
https://www.ncbi.nlm.nih.gov/pubmed/32596148
http://dx.doi.org/10.3389/fonc.2020.00855
_version_ 1783547751309508608
author Wang, Weidong
Chen, Dongni
Chen, Weiwei
Xin, Ziya
Huang, Zirui
Zhang, Xuewen
Xi, Kexing
Wang, Gongming
Zhang, Rusi
Zhao, Dechang
Liu, Li
Zhang, Lanjun
author_facet Wang, Weidong
Chen, Dongni
Chen, Weiwei
Xin, Ziya
Huang, Zirui
Zhang, Xuewen
Xi, Kexing
Wang, Gongming
Zhang, Rusi
Zhao, Dechang
Liu, Li
Zhang, Lanjun
author_sort Wang, Weidong
collection PubMed
description Background: We previously identified a 12-microRNA (miRNA) panel (miRNA-17, miRNA-146a, miRNA-200b, miRNA-182, miRNA-155, miRNA-221, miRNA-205, miRNA-126, miRNA-7, miRNA-21, miRNA-145, and miRNA-210) that aided in the early diagnosis of non-small cell lung cancer (NSCLC). We validated the diagnostic value of this miRNA panel and compared it with that of traditional tumor markers and radiological diagnosis. We constructed a nomogram based on the miRNA panel's results to predict the risk of NSCLC. Methods: Eighty-two participants with pulmonary nodules on a CT scan and who underwent a pathological examination and surgical treatment were enrolled in our study. Patients were randomly divided into a training group or a validation group. The miRNA concentrations were quantified by RT-PCR and log-transformed for analysis. The cutoff value was determined in the training group and then applied in the validation group. A comparison between the miRNAs and traditional tumor markers [CEA, NSE, and cytokeratin 19 fragment 21-1 (Cyfra21-1)] and radiological diagnosis was performed. A nomogram based on the miRNA panel's results to predict the risk of NSCLC was constructed. Results: The expression level of these 12 miRNAs was significantly higher in NSCLC patients than in benign patients. In the validation group, the specificity and positive predictive value were 96.4 and 95.8%, respectively, which were significantly higher than those using traditional tumor markers or radiological diagnosis. The sensitivity was 42.6%, which was also higher than that using tumor markers. Moreover, the sensitivity increased to 63.6% when the nodule diameters were larger than 2 cm. The miRNAs and seven clinical factors were integrated into the nomogram, and the calibration curves showed optimal agreement between the predicted and actual probabilities. Conclusions: Our miRNA panel has clinical value for the early detection of NSCLC. A nomogram was constructed and internally validated, and the results indicate that it can assist clinicians in making treatment recommendations in the clinic.
format Online
Article
Text
id pubmed-7301755
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-73017552020-06-26 Early Detection of Non-Small Cell Lung Cancer by Using a 12-microRNA Panel and a Nomogram for Assistant Diagnosis Wang, Weidong Chen, Dongni Chen, Weiwei Xin, Ziya Huang, Zirui Zhang, Xuewen Xi, Kexing Wang, Gongming Zhang, Rusi Zhao, Dechang Liu, Li Zhang, Lanjun Front Oncol Oncology Background: We previously identified a 12-microRNA (miRNA) panel (miRNA-17, miRNA-146a, miRNA-200b, miRNA-182, miRNA-155, miRNA-221, miRNA-205, miRNA-126, miRNA-7, miRNA-21, miRNA-145, and miRNA-210) that aided in the early diagnosis of non-small cell lung cancer (NSCLC). We validated the diagnostic value of this miRNA panel and compared it with that of traditional tumor markers and radiological diagnosis. We constructed a nomogram based on the miRNA panel's results to predict the risk of NSCLC. Methods: Eighty-two participants with pulmonary nodules on a CT scan and who underwent a pathological examination and surgical treatment were enrolled in our study. Patients were randomly divided into a training group or a validation group. The miRNA concentrations were quantified by RT-PCR and log-transformed for analysis. The cutoff value was determined in the training group and then applied in the validation group. A comparison between the miRNAs and traditional tumor markers [CEA, NSE, and cytokeratin 19 fragment 21-1 (Cyfra21-1)] and radiological diagnosis was performed. A nomogram based on the miRNA panel's results to predict the risk of NSCLC was constructed. Results: The expression level of these 12 miRNAs was significantly higher in NSCLC patients than in benign patients. In the validation group, the specificity and positive predictive value were 96.4 and 95.8%, respectively, which were significantly higher than those using traditional tumor markers or radiological diagnosis. The sensitivity was 42.6%, which was also higher than that using tumor markers. Moreover, the sensitivity increased to 63.6% when the nodule diameters were larger than 2 cm. The miRNAs and seven clinical factors were integrated into the nomogram, and the calibration curves showed optimal agreement between the predicted and actual probabilities. Conclusions: Our miRNA panel has clinical value for the early detection of NSCLC. A nomogram was constructed and internally validated, and the results indicate that it can assist clinicians in making treatment recommendations in the clinic. Frontiers Media S.A. 2020-06-11 /pmc/articles/PMC7301755/ /pubmed/32596148 http://dx.doi.org/10.3389/fonc.2020.00855 Text en Copyright © 2020 Wang, Chen, Chen, Xin, Huang, Zhang, Xi, Wang, Zhang, Zhao, Liu and Zhang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Wang, Weidong
Chen, Dongni
Chen, Weiwei
Xin, Ziya
Huang, Zirui
Zhang, Xuewen
Xi, Kexing
Wang, Gongming
Zhang, Rusi
Zhao, Dechang
Liu, Li
Zhang, Lanjun
Early Detection of Non-Small Cell Lung Cancer by Using a 12-microRNA Panel and a Nomogram for Assistant Diagnosis
title Early Detection of Non-Small Cell Lung Cancer by Using a 12-microRNA Panel and a Nomogram for Assistant Diagnosis
title_full Early Detection of Non-Small Cell Lung Cancer by Using a 12-microRNA Panel and a Nomogram for Assistant Diagnosis
title_fullStr Early Detection of Non-Small Cell Lung Cancer by Using a 12-microRNA Panel and a Nomogram for Assistant Diagnosis
title_full_unstemmed Early Detection of Non-Small Cell Lung Cancer by Using a 12-microRNA Panel and a Nomogram for Assistant Diagnosis
title_short Early Detection of Non-Small Cell Lung Cancer by Using a 12-microRNA Panel and a Nomogram for Assistant Diagnosis
title_sort early detection of non-small cell lung cancer by using a 12-microrna panel and a nomogram for assistant diagnosis
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7301755/
https://www.ncbi.nlm.nih.gov/pubmed/32596148
http://dx.doi.org/10.3389/fonc.2020.00855
work_keys_str_mv AT wangweidong earlydetectionofnonsmallcelllungcancerbyusinga12micrornapanelandanomogramforassistantdiagnosis
AT chendongni earlydetectionofnonsmallcelllungcancerbyusinga12micrornapanelandanomogramforassistantdiagnosis
AT chenweiwei earlydetectionofnonsmallcelllungcancerbyusinga12micrornapanelandanomogramforassistantdiagnosis
AT xinziya earlydetectionofnonsmallcelllungcancerbyusinga12micrornapanelandanomogramforassistantdiagnosis
AT huangzirui earlydetectionofnonsmallcelllungcancerbyusinga12micrornapanelandanomogramforassistantdiagnosis
AT zhangxuewen earlydetectionofnonsmallcelllungcancerbyusinga12micrornapanelandanomogramforassistantdiagnosis
AT xikexing earlydetectionofnonsmallcelllungcancerbyusinga12micrornapanelandanomogramforassistantdiagnosis
AT wanggongming earlydetectionofnonsmallcelllungcancerbyusinga12micrornapanelandanomogramforassistantdiagnosis
AT zhangrusi earlydetectionofnonsmallcelllungcancerbyusinga12micrornapanelandanomogramforassistantdiagnosis
AT zhaodechang earlydetectionofnonsmallcelllungcancerbyusinga12micrornapanelandanomogramforassistantdiagnosis
AT liuli earlydetectionofnonsmallcelllungcancerbyusinga12micrornapanelandanomogramforassistantdiagnosis
AT zhanglanjun earlydetectionofnonsmallcelllungcancerbyusinga12micrornapanelandanomogramforassistantdiagnosis