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Oxidation processes related to seed storage and seedling growth of Malus sylvestris, Prunus avium and Prunus padus

Seeds stored in controlled conditions in gene banks, faster or slower lose their viability. The effects of seed moisture content levels (ca. 5, 8, 11%) combined with storage temperatures (-3°, -18°, -196°C) were investigated in terms of the description of seeds defined as orthodox under oxidative st...

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Autores principales: Wawrzyniak, Mikołaj Krzysztof, Kalemba, Ewa Marzena, Ratajczak, Ewelina, Chmielarz, Paweł
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7302524/
https://www.ncbi.nlm.nih.gov/pubmed/32555619
http://dx.doi.org/10.1371/journal.pone.0234510
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author Wawrzyniak, Mikołaj Krzysztof
Kalemba, Ewa Marzena
Ratajczak, Ewelina
Chmielarz, Paweł
author_facet Wawrzyniak, Mikołaj Krzysztof
Kalemba, Ewa Marzena
Ratajczak, Ewelina
Chmielarz, Paweł
author_sort Wawrzyniak, Mikołaj Krzysztof
collection PubMed
description Seeds stored in controlled conditions in gene banks, faster or slower lose their viability. The effects of seed moisture content levels (ca. 5, 8, 11%) combined with storage temperatures (-3°, -18°, -196°C) were investigated in terms of the description of seeds defined as orthodox under oxidative stress after seed storage, during germination, and initial seedling growth. Hydrogen peroxide (H(2)O(2)), thiobarbituric acid reactive substances (TBARS) and ascorbate (Asc) were analyzed in relation to seed germinability and seedlings emergence in three species: Malus sylvestris L., Prunus avium L. and Prunus padus L. The effect of seed storage conditions on H(2)O(2) levels appeared in germinated seeds after the third year of storage in each species. The H(2)O(2) levels were negatively correlated with the germination and seedling emergence of P. avium seeds after three years of storage under all examined combinations. The emergence of P. padus seedlings was not linked to any of the stress markers tested. The P. padus seed biochemical traits were least altered by storage conditions, and the seeds produced tolerant seedlings of relatively high levels of H(2)O(2) and TBARS. To cope with different H(2)O(2) levels, TBARS levels, and Asc levels in seeds of three species varying storage conditions different molecular responses, i.e. repairing mechanisms, were applied during stratification to compensate for the storage conditions and, as a result, seeds remained viable and seedlings were successfully established.
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spelling pubmed-73025242020-06-19 Oxidation processes related to seed storage and seedling growth of Malus sylvestris, Prunus avium and Prunus padus Wawrzyniak, Mikołaj Krzysztof Kalemba, Ewa Marzena Ratajczak, Ewelina Chmielarz, Paweł PLoS One Research Article Seeds stored in controlled conditions in gene banks, faster or slower lose their viability. The effects of seed moisture content levels (ca. 5, 8, 11%) combined with storage temperatures (-3°, -18°, -196°C) were investigated in terms of the description of seeds defined as orthodox under oxidative stress after seed storage, during germination, and initial seedling growth. Hydrogen peroxide (H(2)O(2)), thiobarbituric acid reactive substances (TBARS) and ascorbate (Asc) were analyzed in relation to seed germinability and seedlings emergence in three species: Malus sylvestris L., Prunus avium L. and Prunus padus L. The effect of seed storage conditions on H(2)O(2) levels appeared in germinated seeds after the third year of storage in each species. The H(2)O(2) levels were negatively correlated with the germination and seedling emergence of P. avium seeds after three years of storage under all examined combinations. The emergence of P. padus seedlings was not linked to any of the stress markers tested. The P. padus seed biochemical traits were least altered by storage conditions, and the seeds produced tolerant seedlings of relatively high levels of H(2)O(2) and TBARS. To cope with different H(2)O(2) levels, TBARS levels, and Asc levels in seeds of three species varying storage conditions different molecular responses, i.e. repairing mechanisms, were applied during stratification to compensate for the storage conditions and, as a result, seeds remained viable and seedlings were successfully established. Public Library of Science 2020-06-18 /pmc/articles/PMC7302524/ /pubmed/32555619 http://dx.doi.org/10.1371/journal.pone.0234510 Text en © 2020 Wawrzyniak et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Wawrzyniak, Mikołaj Krzysztof
Kalemba, Ewa Marzena
Ratajczak, Ewelina
Chmielarz, Paweł
Oxidation processes related to seed storage and seedling growth of Malus sylvestris, Prunus avium and Prunus padus
title Oxidation processes related to seed storage and seedling growth of Malus sylvestris, Prunus avium and Prunus padus
title_full Oxidation processes related to seed storage and seedling growth of Malus sylvestris, Prunus avium and Prunus padus
title_fullStr Oxidation processes related to seed storage and seedling growth of Malus sylvestris, Prunus avium and Prunus padus
title_full_unstemmed Oxidation processes related to seed storage and seedling growth of Malus sylvestris, Prunus avium and Prunus padus
title_short Oxidation processes related to seed storage and seedling growth of Malus sylvestris, Prunus avium and Prunus padus
title_sort oxidation processes related to seed storage and seedling growth of malus sylvestris, prunus avium and prunus padus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7302524/
https://www.ncbi.nlm.nih.gov/pubmed/32555619
http://dx.doi.org/10.1371/journal.pone.0234510
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