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Protein expression-independent response of intensity-based pH-sensitive fluorophores in Escherichia coli
Fluorescent proteins that modulate their emission intensities when protonated serve as excellent probes of the cytosolic pH. Since the total fluorescence output fluctuates significantly due to variations in the fluorophore levels in cells, eliminating the dependence of the signal on protein concentr...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7302705/ https://www.ncbi.nlm.nih.gov/pubmed/32555627 http://dx.doi.org/10.1371/journal.pone.0234849 |