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Near-infrared photoimmunotherapy is effective treatment for colorectal cancer in orthotopic nude-mouse models
BACKGROUND: Photoimmunotherapy (PIT) employs the use of a near-infrared (NIR) laser to activate an antibody conjugated to a NIR-activatable dye to induce cancer cell death. PIT has shown to be effective in a number of studies, however, there are no data on its use in colorectal cancer in an orthotop...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7302706/ https://www.ncbi.nlm.nih.gov/pubmed/32555717 http://dx.doi.org/10.1371/journal.pone.0234643 |
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author | Hollandsworth, Hannah M. Amirfakhri, Siamak Filemoni, Filemoni Molnar, Justin Hoffman, Robert M. Yazaki, Paul Bouvet, Michael |
author_facet | Hollandsworth, Hannah M. Amirfakhri, Siamak Filemoni, Filemoni Molnar, Justin Hoffman, Robert M. Yazaki, Paul Bouvet, Michael |
author_sort | Hollandsworth, Hannah M. |
collection | PubMed |
description | BACKGROUND: Photoimmunotherapy (PIT) employs the use of a near-infrared (NIR) laser to activate an antibody conjugated to a NIR-activatable dye to induce cancer cell death. PIT has shown to be effective in a number of studies, however, there are no data on its use in colorectal cancer in an orthotopic model. METHODS: Humanized anti-CEA antibody (M5A) was conjugated to NIR-activatable IRDye700DX (M5A-700). PIT was validated in vitro with a colon cancer cell-line, using a laser intensity of either 4 J/cm(2), 8 J/cm(2), or 16 J/cm(2). Orthotopic colon cancer mouse models were established by surgical implantation of LS174T tumor fragments onto the cecum. M5A-700 was administered and PIT was performed 24 hours later using a 690 nm laser. Repeat PIT was performed after 7 days in one group. Control mice received laser treatment only. RESULTS: In vitro PIT demonstrated tumor cell death in a laser intensity dose-dependent fashion. In orthotopic models, control mice demonstrated persistent tumor growth. Mice that underwent PIT one time had tumor growth arrested for one week, after which re-growth occurred. The group that received repeated PIT exposure had persistent inhibition of tumor growth. CONCLUSION: PIT arrests tumor growth in colon cancer orthotopic nude-mouse models. Repeated PIT arrests colon cancer growth for a longer period of time. PIT may be a useful therapy in the future as an adjunct to surgical resection or as primary therapy to suppress tumor progression. |
format | Online Article Text |
id | pubmed-7302706 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-73027062020-06-19 Near-infrared photoimmunotherapy is effective treatment for colorectal cancer in orthotopic nude-mouse models Hollandsworth, Hannah M. Amirfakhri, Siamak Filemoni, Filemoni Molnar, Justin Hoffman, Robert M. Yazaki, Paul Bouvet, Michael PLoS One Research Article BACKGROUND: Photoimmunotherapy (PIT) employs the use of a near-infrared (NIR) laser to activate an antibody conjugated to a NIR-activatable dye to induce cancer cell death. PIT has shown to be effective in a number of studies, however, there are no data on its use in colorectal cancer in an orthotopic model. METHODS: Humanized anti-CEA antibody (M5A) was conjugated to NIR-activatable IRDye700DX (M5A-700). PIT was validated in vitro with a colon cancer cell-line, using a laser intensity of either 4 J/cm(2), 8 J/cm(2), or 16 J/cm(2). Orthotopic colon cancer mouse models were established by surgical implantation of LS174T tumor fragments onto the cecum. M5A-700 was administered and PIT was performed 24 hours later using a 690 nm laser. Repeat PIT was performed after 7 days in one group. Control mice received laser treatment only. RESULTS: In vitro PIT demonstrated tumor cell death in a laser intensity dose-dependent fashion. In orthotopic models, control mice demonstrated persistent tumor growth. Mice that underwent PIT one time had tumor growth arrested for one week, after which re-growth occurred. The group that received repeated PIT exposure had persistent inhibition of tumor growth. CONCLUSION: PIT arrests tumor growth in colon cancer orthotopic nude-mouse models. Repeated PIT arrests colon cancer growth for a longer period of time. PIT may be a useful therapy in the future as an adjunct to surgical resection or as primary therapy to suppress tumor progression. Public Library of Science 2020-06-18 /pmc/articles/PMC7302706/ /pubmed/32555717 http://dx.doi.org/10.1371/journal.pone.0234643 Text en © 2020 Hollandsworth et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Hollandsworth, Hannah M. Amirfakhri, Siamak Filemoni, Filemoni Molnar, Justin Hoffman, Robert M. Yazaki, Paul Bouvet, Michael Near-infrared photoimmunotherapy is effective treatment for colorectal cancer in orthotopic nude-mouse models |
title | Near-infrared photoimmunotherapy is effective treatment for colorectal cancer in orthotopic nude-mouse models |
title_full | Near-infrared photoimmunotherapy is effective treatment for colorectal cancer in orthotopic nude-mouse models |
title_fullStr | Near-infrared photoimmunotherapy is effective treatment for colorectal cancer in orthotopic nude-mouse models |
title_full_unstemmed | Near-infrared photoimmunotherapy is effective treatment for colorectal cancer in orthotopic nude-mouse models |
title_short | Near-infrared photoimmunotherapy is effective treatment for colorectal cancer in orthotopic nude-mouse models |
title_sort | near-infrared photoimmunotherapy is effective treatment for colorectal cancer in orthotopic nude-mouse models |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7302706/ https://www.ncbi.nlm.nih.gov/pubmed/32555717 http://dx.doi.org/10.1371/journal.pone.0234643 |
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