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The relationship between clinics and the venom of the causative Amazon pit viper (Bothrops atrox)
Snake venoms are complex mixtures of proteins with toxic activities, with many distinct isoforms, affecting different physiological targets, comprised in a few protein families. It is currently accepted that this diversity in venom composition is an adaptive advantage for venom efficacy on a wide ra...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7302866/ https://www.ncbi.nlm.nih.gov/pubmed/32511239 http://dx.doi.org/10.1371/journal.pntd.0008299 |
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author | Moura-da-Silva, Ana Maria Contreras-Bernal, Jorge Carlos Cirilo Gimenes, Sarah Natalie Freitas-de-Sousa, Luciana Aparecida Portes-Junior, José Antonio da Silva Peixoto, Pedro Kei Iwai, Leo Mourão de Moura, Valéria Ferreira Bisneto, Pedro Lacerda, Marcus Mendonça da Silva, Iran de Lima Ferreira, Luiz Carlos Silva de Oliveira, Sâmella Hui Wen, Fan de Almeida Gonçalves Sachett, Jacqueline Monteiro, Wuelton M. |
author_facet | Moura-da-Silva, Ana Maria Contreras-Bernal, Jorge Carlos Cirilo Gimenes, Sarah Natalie Freitas-de-Sousa, Luciana Aparecida Portes-Junior, José Antonio da Silva Peixoto, Pedro Kei Iwai, Leo Mourão de Moura, Valéria Ferreira Bisneto, Pedro Lacerda, Marcus Mendonça da Silva, Iran de Lima Ferreira, Luiz Carlos Silva de Oliveira, Sâmella Hui Wen, Fan de Almeida Gonçalves Sachett, Jacqueline Monteiro, Wuelton M. |
author_sort | Moura-da-Silva, Ana Maria |
collection | PubMed |
description | Snake venoms are complex mixtures of proteins with toxic activities, with many distinct isoforms, affecting different physiological targets, comprised in a few protein families. It is currently accepted that this diversity in venom composition is an adaptive advantage for venom efficacy on a wide range of prey. However, on the other side, variability on isoforms expression has implications in the clinics of human victims of snakebites and in the efficacy of antivenoms. B. atrox snakes are responsible for most of the human accidents in Brazilian Amazon and the type and abundance of protein families on their venoms present individual variability. Thus, in this study we attempted to correlate the individual venom proteome of the snake brought to the hospital by the patient seeking for medical assistance with the clinical signs observed in the same patient. Individual variability was confirmed in venoms of the 14 snakes selected for the study. The abundance of each protein family was quite similar among the venom samples, while the isoforms composition was highly variable. Considering the protein families, the SVMP group presented the best correlation with bleeding disorders and edema. Considering individual isoforms, some isoforms of venom metalloproteinase (SVMP), C-type lectin-like toxins (CTL) and snake venom serine proteinases (SVSP) presented expression levels that with statistically significant positive correlation to signs and symptoms presented by the patients as bleeding disorders, edema, ecchymosis and blister formation. However, some unexpected data were also observed as the correlation between a CTL, CRISP or LAAO isoforms with blister formation, still to be confirmed with a larger number of samples. Although this is still a small number of patient samples, we were able to indicate that venom composition modulates clinical manifestations of snakebites, to confirm at the bedside the prominent role of SVMPs and to include new possible toxin candidates for the development of toxin inhibitors or to improve antivenom selectiveness, important actions for the next generation treatments of snakebites. |
format | Online Article Text |
id | pubmed-7302866 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-73028662020-06-19 The relationship between clinics and the venom of the causative Amazon pit viper (Bothrops atrox) Moura-da-Silva, Ana Maria Contreras-Bernal, Jorge Carlos Cirilo Gimenes, Sarah Natalie Freitas-de-Sousa, Luciana Aparecida Portes-Junior, José Antonio da Silva Peixoto, Pedro Kei Iwai, Leo Mourão de Moura, Valéria Ferreira Bisneto, Pedro Lacerda, Marcus Mendonça da Silva, Iran de Lima Ferreira, Luiz Carlos Silva de Oliveira, Sâmella Hui Wen, Fan de Almeida Gonçalves Sachett, Jacqueline Monteiro, Wuelton M. PLoS Negl Trop Dis Research Article Snake venoms are complex mixtures of proteins with toxic activities, with many distinct isoforms, affecting different physiological targets, comprised in a few protein families. It is currently accepted that this diversity in venom composition is an adaptive advantage for venom efficacy on a wide range of prey. However, on the other side, variability on isoforms expression has implications in the clinics of human victims of snakebites and in the efficacy of antivenoms. B. atrox snakes are responsible for most of the human accidents in Brazilian Amazon and the type and abundance of protein families on their venoms present individual variability. Thus, in this study we attempted to correlate the individual venom proteome of the snake brought to the hospital by the patient seeking for medical assistance with the clinical signs observed in the same patient. Individual variability was confirmed in venoms of the 14 snakes selected for the study. The abundance of each protein family was quite similar among the venom samples, while the isoforms composition was highly variable. Considering the protein families, the SVMP group presented the best correlation with bleeding disorders and edema. Considering individual isoforms, some isoforms of venom metalloproteinase (SVMP), C-type lectin-like toxins (CTL) and snake venom serine proteinases (SVSP) presented expression levels that with statistically significant positive correlation to signs and symptoms presented by the patients as bleeding disorders, edema, ecchymosis and blister formation. However, some unexpected data were also observed as the correlation between a CTL, CRISP or LAAO isoforms with blister formation, still to be confirmed with a larger number of samples. Although this is still a small number of patient samples, we were able to indicate that venom composition modulates clinical manifestations of snakebites, to confirm at the bedside the prominent role of SVMPs and to include new possible toxin candidates for the development of toxin inhibitors or to improve antivenom selectiveness, important actions for the next generation treatments of snakebites. Public Library of Science 2020-06-08 /pmc/articles/PMC7302866/ /pubmed/32511239 http://dx.doi.org/10.1371/journal.pntd.0008299 Text en © 2020 Moura-da-Silva et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Moura-da-Silva, Ana Maria Contreras-Bernal, Jorge Carlos Cirilo Gimenes, Sarah Natalie Freitas-de-Sousa, Luciana Aparecida Portes-Junior, José Antonio da Silva Peixoto, Pedro Kei Iwai, Leo Mourão de Moura, Valéria Ferreira Bisneto, Pedro Lacerda, Marcus Mendonça da Silva, Iran de Lima Ferreira, Luiz Carlos Silva de Oliveira, Sâmella Hui Wen, Fan de Almeida Gonçalves Sachett, Jacqueline Monteiro, Wuelton M. The relationship between clinics and the venom of the causative Amazon pit viper (Bothrops atrox) |
title | The relationship between clinics and the venom of the causative Amazon pit viper (Bothrops atrox) |
title_full | The relationship between clinics and the venom of the causative Amazon pit viper (Bothrops atrox) |
title_fullStr | The relationship between clinics and the venom of the causative Amazon pit viper (Bothrops atrox) |
title_full_unstemmed | The relationship between clinics and the venom of the causative Amazon pit viper (Bothrops atrox) |
title_short | The relationship between clinics and the venom of the causative Amazon pit viper (Bothrops atrox) |
title_sort | relationship between clinics and the venom of the causative amazon pit viper (bothrops atrox) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7302866/ https://www.ncbi.nlm.nih.gov/pubmed/32511239 http://dx.doi.org/10.1371/journal.pntd.0008299 |
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