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Constructing arrays of nucleosome positioning sequences using Gibson Assembly for single-molecule studies

As the basic building blocks of chromatin, nucleosomes play a key role in dictating the accessibility of the eukaryotic genome. Consequently, nucleosomes are involved in essential genomic transactions such as DNA transcription, replication and repair. In order to unravel the mechanisms by which nucl...

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Detalles Bibliográficos
Autores principales: Spakman, Dian, King, Graeme A., Peterman, Erwin J. G., Wuite, Gijs J. L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7303147/
https://www.ncbi.nlm.nih.gov/pubmed/32555215
http://dx.doi.org/10.1038/s41598-020-66259-4