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Critical Role of Type III Interferon in Controlling SARS-CoV-2 Infection in Human Intestinal Epithelial Cells
Severe acute respiratory syndrome-related coronavirus-2 (SARS-CoV-2) is an unprecedented worldwide health problem that requires concerted and global approaches to stop the coronavirus 2019 (COVID-19) pandemic. Although SARS-CoV-2 primarily targets lung epithelium cells, there is growing evidence tha...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cell Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7303637/ https://www.ncbi.nlm.nih.gov/pubmed/32610043 http://dx.doi.org/10.1016/j.celrep.2020.107863 |
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author | Stanifer, Megan L. Kee, Carmon Cortese, Mirko Zumaran, Camila Metz Triana, Sergio Mukenhirn, Markus Kraeusslich, Hans-Georg Alexandrov, Theodore Bartenschlager, Ralf Boulant, Steeve |
author_facet | Stanifer, Megan L. Kee, Carmon Cortese, Mirko Zumaran, Camila Metz Triana, Sergio Mukenhirn, Markus Kraeusslich, Hans-Georg Alexandrov, Theodore Bartenschlager, Ralf Boulant, Steeve |
author_sort | Stanifer, Megan L. |
collection | PubMed |
description | Severe acute respiratory syndrome-related coronavirus-2 (SARS-CoV-2) is an unprecedented worldwide health problem that requires concerted and global approaches to stop the coronavirus 2019 (COVID-19) pandemic. Although SARS-CoV-2 primarily targets lung epithelium cells, there is growing evidence that the intestinal epithelium is also infected. Here, using both colon-derived cell lines and primary non-transformed colon organoids, we engage in the first comprehensive analysis of the SARS-CoV-2 life cycle in human intestinal epithelial cells (hIECs). Our results demonstrate that hIECs fully support SARS-CoV-2 infection, replication, and production of infectious de novo virus particles. We found that viral infection elicits an extremely robust intrinsic immune response where interferon-mediated responses are efficient at controlling SARS-CoV-2 replication and de novo virus production. Taken together, our data demonstrate that hIECs are a productive site of SARS-CoV-2 replication and suggest that the enteric phase of SARS-CoV-2 may participate in the pathologies observed in COVID-19 patients by contributing to increasing patient viremia and fueling an exacerbated cytokine response. |
format | Online Article Text |
id | pubmed-7303637 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Cell Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-73036372020-06-19 Critical Role of Type III Interferon in Controlling SARS-CoV-2 Infection in Human Intestinal Epithelial Cells Stanifer, Megan L. Kee, Carmon Cortese, Mirko Zumaran, Camila Metz Triana, Sergio Mukenhirn, Markus Kraeusslich, Hans-Georg Alexandrov, Theodore Bartenschlager, Ralf Boulant, Steeve Cell Rep Article Severe acute respiratory syndrome-related coronavirus-2 (SARS-CoV-2) is an unprecedented worldwide health problem that requires concerted and global approaches to stop the coronavirus 2019 (COVID-19) pandemic. Although SARS-CoV-2 primarily targets lung epithelium cells, there is growing evidence that the intestinal epithelium is also infected. Here, using both colon-derived cell lines and primary non-transformed colon organoids, we engage in the first comprehensive analysis of the SARS-CoV-2 life cycle in human intestinal epithelial cells (hIECs). Our results demonstrate that hIECs fully support SARS-CoV-2 infection, replication, and production of infectious de novo virus particles. We found that viral infection elicits an extremely robust intrinsic immune response where interferon-mediated responses are efficient at controlling SARS-CoV-2 replication and de novo virus production. Taken together, our data demonstrate that hIECs are a productive site of SARS-CoV-2 replication and suggest that the enteric phase of SARS-CoV-2 may participate in the pathologies observed in COVID-19 patients by contributing to increasing patient viremia and fueling an exacerbated cytokine response. Cell Press 2020-06-19 /pmc/articles/PMC7303637/ /pubmed/32610043 http://dx.doi.org/10.1016/j.celrep.2020.107863 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Stanifer, Megan L. Kee, Carmon Cortese, Mirko Zumaran, Camila Metz Triana, Sergio Mukenhirn, Markus Kraeusslich, Hans-Georg Alexandrov, Theodore Bartenschlager, Ralf Boulant, Steeve Critical Role of Type III Interferon in Controlling SARS-CoV-2 Infection in Human Intestinal Epithelial Cells |
title | Critical Role of Type III Interferon in Controlling SARS-CoV-2 Infection in Human Intestinal Epithelial Cells |
title_full | Critical Role of Type III Interferon in Controlling SARS-CoV-2 Infection in Human Intestinal Epithelial Cells |
title_fullStr | Critical Role of Type III Interferon in Controlling SARS-CoV-2 Infection in Human Intestinal Epithelial Cells |
title_full_unstemmed | Critical Role of Type III Interferon in Controlling SARS-CoV-2 Infection in Human Intestinal Epithelial Cells |
title_short | Critical Role of Type III Interferon in Controlling SARS-CoV-2 Infection in Human Intestinal Epithelial Cells |
title_sort | critical role of type iii interferon in controlling sars-cov-2 infection in human intestinal epithelial cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7303637/ https://www.ncbi.nlm.nih.gov/pubmed/32610043 http://dx.doi.org/10.1016/j.celrep.2020.107863 |
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