Induced pluripotent stem cell-derived smooth muscle cells increase angiogenesis and accelerate diabetic wound healing

AIM: To assess the potential of human induced pluripotent stem cell-derived smooth muscle cells (hiPSC-SMC) to accelerate diabetic wound healing. METHODS: hiPSC-SMC were embedded in 3D collagen scaffolds and cultured in vitro for 72 h; scaffolds were then applied to diabetic, nude mouse, splinted ba...

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Detalles Bibliográficos
Autores principales: Gorecka, Jolanta, Gao, Xixiang, Fereydooni, Arash, Dash, Biraja C, Luo, Jiesi, Lee, Shin Rong, Taniguchi, Ryosuke, Hsia, Henry C, Qyang, Yibing, Dardik, Alan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Medicine Ltd 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7304438/
https://www.ncbi.nlm.nih.gov/pubmed/32228292
http://dx.doi.org/10.2217/rme-2019-0086
Descripción
Sumario:AIM: To assess the potential of human induced pluripotent stem cell-derived smooth muscle cells (hiPSC-SMC) to accelerate diabetic wound healing. METHODS: hiPSC-SMC were embedded in 3D collagen scaffolds and cultured in vitro for 72 h; scaffolds were then applied to diabetic, nude mouse, splinted back wounds to assess in vivo healing. Cultured medium after scaffold incubation was collected and analyzed for expression of pro-angiogenic cytokines. RESULTS: hiPSC-SMC secrete increased concentration of pro-angiogenic cytokines, compared with murine adipose derived stem cells. Delivery of hiPSC-SMC-containing collagen scaffolds accelerates diabetic wound healing and is associated with an increased number of total and M2 type macrophages. CONCLUSION: hiPSC-SMC promote angiogenesis and accelerate diabetic wound healing, making them a promising new candidate for treatment of diabetic wounds.

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