Cargando…

Role of Dopaminergic Receptors Within the Ventral Tegmental Area in Antinociception Induced by Chemical Stimulation of the Lateral Hypothalamus in an Animal Model of Orofacial Pain

INTRODUCTION: The ventral tegmental area (VTA), as one of the classical components of the brain reward circuitry, shares large neural networks with the pain processing system. We previously showed the role of VTA dopamine receptors in modulation of lateral hypothalamus (LH)-induced antinociception i...

Descripción completa

Detalles Bibliográficos
Autores principales: Matini, Tina, Haghparast, Amir, Rezaee, Laleh, Salehi, Sakineh, Tehranchi, Azita, Haghparast, Abbas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7304680/
https://www.ncbi.nlm.nih.gov/pubmed/32606911
http://dx.doi.org/10.2147/JPR.S255250
_version_ 1783548303012528128
author Matini, Tina
Haghparast, Amir
Rezaee, Laleh
Salehi, Sakineh
Tehranchi, Azita
Haghparast, Abbas
author_facet Matini, Tina
Haghparast, Amir
Rezaee, Laleh
Salehi, Sakineh
Tehranchi, Azita
Haghparast, Abbas
author_sort Matini, Tina
collection PubMed
description INTRODUCTION: The ventral tegmental area (VTA), as one of the classical components of the brain reward circuitry, shares large neural networks with the pain processing system. We previously showed the role of VTA dopamine receptors in modulation of lateral hypothalamus (LH)-induced antinociception in acute pain conditions. However, considering the fact that the neural systems involved in the mediation of tonic pain are not the same as those that mediate phasic pain. In the present study, we aimed to examine the role of intra-VTA dopamine receptors in LH-induced antinociceptive responses during tonic orofacial pain conditions. METHODS: Male Wistar rats weighing 230–250 g were implanted with two separate cannulae into the LH and VTA on the same side. Different solutions of carbachol (62.5, 125 and 250 nM), as a non-selective cholinergic receptor agonist that activates the LH projecting neurons, were microinjected into the LH. In the other groups, D1-like dopamine receptor antagonist, SCH-23390 (0.25, 1 and 4 µg/03 µL saline) or D2-like dopamine receptor antagonist, Sulpiride (0.25, 1 and 4 µg/0.3 µL DMSO 12%) were microinjected into VTA, 5 min prior intra-LH carbachol (250 nM), then subjected to orofacial formalin test. Intra-LH carbachol microinjection dose-dependently attenuated biphasic orofacial pain. RESULTS: Intra-VTA administration of SCH-23390 or Sulpiride dose-dependently decreased intra-LH carbachol-induced antinociception during both phases of orofacial formalin test with further effects in the late phase. DISCUSSION: The findings suggest that chemical stimulation of the LH by carbachol possibly activates the orexin projecting neurons and subsequently, the VTA dopaminergic neurons involved in the orofacial pain modulation. Detecting such neural circuitry offers an alternative approach in the development of more efficient therapies for such debilitating pain conditions.
format Online
Article
Text
id pubmed-7304680
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Dove
record_format MEDLINE/PubMed
spelling pubmed-73046802020-06-29 Role of Dopaminergic Receptors Within the Ventral Tegmental Area in Antinociception Induced by Chemical Stimulation of the Lateral Hypothalamus in an Animal Model of Orofacial Pain Matini, Tina Haghparast, Amir Rezaee, Laleh Salehi, Sakineh Tehranchi, Azita Haghparast, Abbas J Pain Res Original Research INTRODUCTION: The ventral tegmental area (VTA), as one of the classical components of the brain reward circuitry, shares large neural networks with the pain processing system. We previously showed the role of VTA dopamine receptors in modulation of lateral hypothalamus (LH)-induced antinociception in acute pain conditions. However, considering the fact that the neural systems involved in the mediation of tonic pain are not the same as those that mediate phasic pain. In the present study, we aimed to examine the role of intra-VTA dopamine receptors in LH-induced antinociceptive responses during tonic orofacial pain conditions. METHODS: Male Wistar rats weighing 230–250 g were implanted with two separate cannulae into the LH and VTA on the same side. Different solutions of carbachol (62.5, 125 and 250 nM), as a non-selective cholinergic receptor agonist that activates the LH projecting neurons, were microinjected into the LH. In the other groups, D1-like dopamine receptor antagonist, SCH-23390 (0.25, 1 and 4 µg/03 µL saline) or D2-like dopamine receptor antagonist, Sulpiride (0.25, 1 and 4 µg/0.3 µL DMSO 12%) were microinjected into VTA, 5 min prior intra-LH carbachol (250 nM), then subjected to orofacial formalin test. Intra-LH carbachol microinjection dose-dependently attenuated biphasic orofacial pain. RESULTS: Intra-VTA administration of SCH-23390 or Sulpiride dose-dependently decreased intra-LH carbachol-induced antinociception during both phases of orofacial formalin test with further effects in the late phase. DISCUSSION: The findings suggest that chemical stimulation of the LH by carbachol possibly activates the orexin projecting neurons and subsequently, the VTA dopaminergic neurons involved in the orofacial pain modulation. Detecting such neural circuitry offers an alternative approach in the development of more efficient therapies for such debilitating pain conditions. Dove 2020-06-15 /pmc/articles/PMC7304680/ /pubmed/32606911 http://dx.doi.org/10.2147/JPR.S255250 Text en © 2020 Matini et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Matini, Tina
Haghparast, Amir
Rezaee, Laleh
Salehi, Sakineh
Tehranchi, Azita
Haghparast, Abbas
Role of Dopaminergic Receptors Within the Ventral Tegmental Area in Antinociception Induced by Chemical Stimulation of the Lateral Hypothalamus in an Animal Model of Orofacial Pain
title Role of Dopaminergic Receptors Within the Ventral Tegmental Area in Antinociception Induced by Chemical Stimulation of the Lateral Hypothalamus in an Animal Model of Orofacial Pain
title_full Role of Dopaminergic Receptors Within the Ventral Tegmental Area in Antinociception Induced by Chemical Stimulation of the Lateral Hypothalamus in an Animal Model of Orofacial Pain
title_fullStr Role of Dopaminergic Receptors Within the Ventral Tegmental Area in Antinociception Induced by Chemical Stimulation of the Lateral Hypothalamus in an Animal Model of Orofacial Pain
title_full_unstemmed Role of Dopaminergic Receptors Within the Ventral Tegmental Area in Antinociception Induced by Chemical Stimulation of the Lateral Hypothalamus in an Animal Model of Orofacial Pain
title_short Role of Dopaminergic Receptors Within the Ventral Tegmental Area in Antinociception Induced by Chemical Stimulation of the Lateral Hypothalamus in an Animal Model of Orofacial Pain
title_sort role of dopaminergic receptors within the ventral tegmental area in antinociception induced by chemical stimulation of the lateral hypothalamus in an animal model of orofacial pain
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7304680/
https://www.ncbi.nlm.nih.gov/pubmed/32606911
http://dx.doi.org/10.2147/JPR.S255250
work_keys_str_mv AT matinitina roleofdopaminergicreceptorswithintheventraltegmentalareainantinociceptioninducedbychemicalstimulationofthelateralhypothalamusinananimalmodeloforofacialpain
AT haghparastamir roleofdopaminergicreceptorswithintheventraltegmentalareainantinociceptioninducedbychemicalstimulationofthelateralhypothalamusinananimalmodeloforofacialpain
AT rezaeelaleh roleofdopaminergicreceptorswithintheventraltegmentalareainantinociceptioninducedbychemicalstimulationofthelateralhypothalamusinananimalmodeloforofacialpain
AT salehisakineh roleofdopaminergicreceptorswithintheventraltegmentalareainantinociceptioninducedbychemicalstimulationofthelateralhypothalamusinananimalmodeloforofacialpain
AT tehranchiazita roleofdopaminergicreceptorswithintheventraltegmentalareainantinociceptioninducedbychemicalstimulationofthelateralhypothalamusinananimalmodeloforofacialpain
AT haghparastabbas roleofdopaminergicreceptorswithintheventraltegmentalareainantinociceptioninducedbychemicalstimulationofthelateralhypothalamusinananimalmodeloforofacialpain