hFRUIT: An optimized agent for optical clearing of DiI-stained adult human brain tissue

Here, we describe a new immersion-based clearing method suitable for optical clearing of thick adult human brain samples while preserving its lipids and lipophilic labels such as 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI). This clearing procedure is simple, easy to impl...

Descripción completa

Detalles Bibliográficos
Autores principales: Hildebrand, Sven, Schueth, Anna, Wangenheim, Klaus von, Mattheyer, Christian, Pampaloni, Francesco, Bratzke, Hansjürgen, Roebroeck, Alard F., Galuske, Ralf A. W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7305111/
https://www.ncbi.nlm.nih.gov/pubmed/32561795
http://dx.doi.org/10.1038/s41598-020-66999-3
_version_ 1783548389645877248
author Hildebrand, Sven
Schueth, Anna
Wangenheim, Klaus von
Mattheyer, Christian
Pampaloni, Francesco
Bratzke, Hansjürgen
Roebroeck, Alard F.
Galuske, Ralf A. W.
author_facet Hildebrand, Sven
Schueth, Anna
Wangenheim, Klaus von
Mattheyer, Christian
Pampaloni, Francesco
Bratzke, Hansjürgen
Roebroeck, Alard F.
Galuske, Ralf A. W.
author_sort Hildebrand, Sven
collection PubMed
description Here, we describe a new immersion-based clearing method suitable for optical clearing of thick adult human brain samples while preserving its lipids and lipophilic labels such as 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI). This clearing procedure is simple, easy to implement, and allowed for clearing of 5 mm thick human brain tissue samples within 12 days. Furthermore, we show for the first time the advantageous effect of the Periodate-Lysine-Paraformaldehyde (PLP) fixation as compared to the more commonly used 4% paraformaldehyde (PFA) on clearing performance.
format Online
Article
Text
id pubmed-7305111
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-73051112020-06-22 hFRUIT: An optimized agent for optical clearing of DiI-stained adult human brain tissue Hildebrand, Sven Schueth, Anna Wangenheim, Klaus von Mattheyer, Christian Pampaloni, Francesco Bratzke, Hansjürgen Roebroeck, Alard F. Galuske, Ralf A. W. Sci Rep Article Here, we describe a new immersion-based clearing method suitable for optical clearing of thick adult human brain samples while preserving its lipids and lipophilic labels such as 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI). This clearing procedure is simple, easy to implement, and allowed for clearing of 5 mm thick human brain tissue samples within 12 days. Furthermore, we show for the first time the advantageous effect of the Periodate-Lysine-Paraformaldehyde (PLP) fixation as compared to the more commonly used 4% paraformaldehyde (PFA) on clearing performance. Nature Publishing Group UK 2020-06-19 /pmc/articles/PMC7305111/ /pubmed/32561795 http://dx.doi.org/10.1038/s41598-020-66999-3 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Hildebrand, Sven
Schueth, Anna
Wangenheim, Klaus von
Mattheyer, Christian
Pampaloni, Francesco
Bratzke, Hansjürgen
Roebroeck, Alard F.
Galuske, Ralf A. W.
hFRUIT: An optimized agent for optical clearing of DiI-stained adult human brain tissue
title hFRUIT: An optimized agent for optical clearing of DiI-stained adult human brain tissue
title_full hFRUIT: An optimized agent for optical clearing of DiI-stained adult human brain tissue
title_fullStr hFRUIT: An optimized agent for optical clearing of DiI-stained adult human brain tissue
title_full_unstemmed hFRUIT: An optimized agent for optical clearing of DiI-stained adult human brain tissue
title_short hFRUIT: An optimized agent for optical clearing of DiI-stained adult human brain tissue
title_sort hfruit: an optimized agent for optical clearing of dii-stained adult human brain tissue
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7305111/
https://www.ncbi.nlm.nih.gov/pubmed/32561795
http://dx.doi.org/10.1038/s41598-020-66999-3
work_keys_str_mv AT hildebrandsven hfruitanoptimizedagentforopticalclearingofdiistainedadulthumanbraintissue
AT schuethanna hfruitanoptimizedagentforopticalclearingofdiistainedadulthumanbraintissue
AT wangenheimklausvon hfruitanoptimizedagentforopticalclearingofdiistainedadulthumanbraintissue
AT mattheyerchristian hfruitanoptimizedagentforopticalclearingofdiistainedadulthumanbraintissue
AT pampalonifrancesco hfruitanoptimizedagentforopticalclearingofdiistainedadulthumanbraintissue
AT bratzkehansjurgen hfruitanoptimizedagentforopticalclearingofdiistainedadulthumanbraintissue
AT roebroeckalardf hfruitanoptimizedagentforopticalclearingofdiistainedadulthumanbraintissue
AT galuskeralfaw hfruitanoptimizedagentforopticalclearingofdiistainedadulthumanbraintissue

Ejemplares similares