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Effect of LINC00657 on Apoptosis of Breast Cancer Cells by Regulating miR-590-3p

OBJECTIVE: To investigate the effect of LINC00657 on breast carcinoma by regulating miR-590-3p. METHODS: Ninety-seven cases with breast carcinoma who were admitted to Qingdao Chengyang People’s Hospital were collected. The breast carcinoma (n=97) and tumor-adjacent tissues (n=97) of patients were co...

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Autores principales: Shan, Qiuli, Qu, Fan, Yang, Weiping, Chen, Ningning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7305342/
https://www.ncbi.nlm.nih.gov/pubmed/32606949
http://dx.doi.org/10.2147/CMAR.S249576
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author Shan, Qiuli
Qu, Fan
Yang, Weiping
Chen, Ningning
author_facet Shan, Qiuli
Qu, Fan
Yang, Weiping
Chen, Ningning
author_sort Shan, Qiuli
collection PubMed
description OBJECTIVE: To investigate the effect of LINC00657 on breast carcinoma by regulating miR-590-3p. METHODS: Ninety-seven cases with breast carcinoma who were admitted to Qingdao Chengyang People’s Hospital were collected. The breast carcinoma (n=97) and tumor-adjacent tissues (n=97) of patients were collected during the operation with the permission of the patients. The expressions of LINC00657 and miR-590-3p were detected in breast carcinoma cells and tissues. The breast carcinoma cells were transfected and their proliferation, migration, invasion and apoptosis were detected. RESULTS: LINC00657 was highly expressed in breast carcinoma tissues, while miR-590-3p was reduced (P<0.05). The proliferation, invasion and migration of cells transfected with si-LINC00657 or miR-590-3p-mimics were significantly inhibited, and the apoptosis rate increased, resulting in the up-regulation of the expressions of apoptosis-related proteins Bax and Caspase-3 and the reduction of Bcl-2 (P<0.05). After si-LINC00657 or miR-590-3p-mimics, the level of GOLPH3 decreased. Through double luciferase report and RIP experiment, it was confirmed that LINC00657 could act as a sponge of miR-590-3p to negatively regulate its expression. After correlation analysis, it was concluded that there was a negative correlation between LINC00657 and miR-590-3p. Rescue experiments concluded that co-transfection of si-LINC00657+miR-590-3P-inhibitor could reverse the inhibitory action of si-LINC00657 on breast carcinoma cells. CONCLUSION: LINC00657 can participate in the biological behavior process of breast carcinoma by regulating miR-590-3p/GOLPH3 signal.
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spelling pubmed-73053422020-06-29 Effect of LINC00657 on Apoptosis of Breast Cancer Cells by Regulating miR-590-3p Shan, Qiuli Qu, Fan Yang, Weiping Chen, Ningning Cancer Manag Res Original Research OBJECTIVE: To investigate the effect of LINC00657 on breast carcinoma by regulating miR-590-3p. METHODS: Ninety-seven cases with breast carcinoma who were admitted to Qingdao Chengyang People’s Hospital were collected. The breast carcinoma (n=97) and tumor-adjacent tissues (n=97) of patients were collected during the operation with the permission of the patients. The expressions of LINC00657 and miR-590-3p were detected in breast carcinoma cells and tissues. The breast carcinoma cells were transfected and their proliferation, migration, invasion and apoptosis were detected. RESULTS: LINC00657 was highly expressed in breast carcinoma tissues, while miR-590-3p was reduced (P<0.05). The proliferation, invasion and migration of cells transfected with si-LINC00657 or miR-590-3p-mimics were significantly inhibited, and the apoptosis rate increased, resulting in the up-regulation of the expressions of apoptosis-related proteins Bax and Caspase-3 and the reduction of Bcl-2 (P<0.05). After si-LINC00657 or miR-590-3p-mimics, the level of GOLPH3 decreased. Through double luciferase report and RIP experiment, it was confirmed that LINC00657 could act as a sponge of miR-590-3p to negatively regulate its expression. After correlation analysis, it was concluded that there was a negative correlation between LINC00657 and miR-590-3p. Rescue experiments concluded that co-transfection of si-LINC00657+miR-590-3P-inhibitor could reverse the inhibitory action of si-LINC00657 on breast carcinoma cells. CONCLUSION: LINC00657 can participate in the biological behavior process of breast carcinoma by regulating miR-590-3p/GOLPH3 signal. Dove 2020-06-15 /pmc/articles/PMC7305342/ /pubmed/32606949 http://dx.doi.org/10.2147/CMAR.S249576 Text en © 2020 Shan et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Shan, Qiuli
Qu, Fan
Yang, Weiping
Chen, Ningning
Effect of LINC00657 on Apoptosis of Breast Cancer Cells by Regulating miR-590-3p
title Effect of LINC00657 on Apoptosis of Breast Cancer Cells by Regulating miR-590-3p
title_full Effect of LINC00657 on Apoptosis of Breast Cancer Cells by Regulating miR-590-3p
title_fullStr Effect of LINC00657 on Apoptosis of Breast Cancer Cells by Regulating miR-590-3p
title_full_unstemmed Effect of LINC00657 on Apoptosis of Breast Cancer Cells by Regulating miR-590-3p
title_short Effect of LINC00657 on Apoptosis of Breast Cancer Cells by Regulating miR-590-3p
title_sort effect of linc00657 on apoptosis of breast cancer cells by regulating mir-590-3p
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7305342/
https://www.ncbi.nlm.nih.gov/pubmed/32606949
http://dx.doi.org/10.2147/CMAR.S249576
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