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Practical considerations for reducing mortality rates in alloxan-induced diabetic rabbits

Chemically-induced diabetic animal models have been employed in many areas of diabetes mellitus (DM) research, but managing post-induction animal survival rates remains one of the main downsides. The aim of the present study was to propose a reliable approach to animal management and monitoring afte...

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Autores principales: Bacevic, Miljana, Rompen, Eric, Radermecker, Regis, Drion, Pierre, Lambert, France
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7305394/
https://www.ncbi.nlm.nih.gov/pubmed/32577551
http://dx.doi.org/10.1016/j.heliyon.2020.e04103
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author Bacevic, Miljana
Rompen, Eric
Radermecker, Regis
Drion, Pierre
Lambert, France
author_facet Bacevic, Miljana
Rompen, Eric
Radermecker, Regis
Drion, Pierre
Lambert, France
author_sort Bacevic, Miljana
collection PubMed
description Chemically-induced diabetic animal models have been employed in many areas of diabetes mellitus (DM) research, but managing post-induction animal survival rates remains one of the main downsides. The aim of the present study was to propose a reliable approach to animal management and monitoring after DM induction in a rabbit model in order to reduce animal mortality rates. DM was induced by injecting alloxan in 12 New Zealand White rabbits. A preventive subcutaneous glucose administration to counteract a potentially lethal hypoglycemic phase following alloxan injection was performed on individual bases. Blood glucose level (BGL) was checked hourly for the first 36 h, then every 2 h until the hyperglycemic state was confirmed. All 12 rabbits survived a 48-hour post-induction phase. The critical hypoglycemic phase's start points and duration differed significantly among the rabbits, lasting from 6.7 to 37 h (19.75 ± 8.44). The rabbits entered the final hyperglycemic phase 18 h at the earliest and 42 h at the latest after induction (26.63 ± 7.07). The average daily BGLs throughout the study period ranged from 268 to 512 mg/dL (413.73 ± 76.69). Eleven rabbits survived until the end of the experiment. The variability of rabbits' responses to alloxan injection emphasizes the importance of monitoring rabbit behavior and thoroughly checking BGLs, followed by a preventive glucose administration based on rabbits' individual needs for up to 36 h after alloxan injection. The proposed approach seems to reduce animal mortality.
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spelling pubmed-73053942020-06-22 Practical considerations for reducing mortality rates in alloxan-induced diabetic rabbits Bacevic, Miljana Rompen, Eric Radermecker, Regis Drion, Pierre Lambert, France Heliyon Article Chemically-induced diabetic animal models have been employed in many areas of diabetes mellitus (DM) research, but managing post-induction animal survival rates remains one of the main downsides. The aim of the present study was to propose a reliable approach to animal management and monitoring after DM induction in a rabbit model in order to reduce animal mortality rates. DM was induced by injecting alloxan in 12 New Zealand White rabbits. A preventive subcutaneous glucose administration to counteract a potentially lethal hypoglycemic phase following alloxan injection was performed on individual bases. Blood glucose level (BGL) was checked hourly for the first 36 h, then every 2 h until the hyperglycemic state was confirmed. All 12 rabbits survived a 48-hour post-induction phase. The critical hypoglycemic phase's start points and duration differed significantly among the rabbits, lasting from 6.7 to 37 h (19.75 ± 8.44). The rabbits entered the final hyperglycemic phase 18 h at the earliest and 42 h at the latest after induction (26.63 ± 7.07). The average daily BGLs throughout the study period ranged from 268 to 512 mg/dL (413.73 ± 76.69). Eleven rabbits survived until the end of the experiment. The variability of rabbits' responses to alloxan injection emphasizes the importance of monitoring rabbit behavior and thoroughly checking BGLs, followed by a preventive glucose administration based on rabbits' individual needs for up to 36 h after alloxan injection. The proposed approach seems to reduce animal mortality. Elsevier 2020-06-17 /pmc/articles/PMC7305394/ /pubmed/32577551 http://dx.doi.org/10.1016/j.heliyon.2020.e04103 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Bacevic, Miljana
Rompen, Eric
Radermecker, Regis
Drion, Pierre
Lambert, France
Practical considerations for reducing mortality rates in alloxan-induced diabetic rabbits
title Practical considerations for reducing mortality rates in alloxan-induced diabetic rabbits
title_full Practical considerations for reducing mortality rates in alloxan-induced diabetic rabbits
title_fullStr Practical considerations for reducing mortality rates in alloxan-induced diabetic rabbits
title_full_unstemmed Practical considerations for reducing mortality rates in alloxan-induced diabetic rabbits
title_short Practical considerations for reducing mortality rates in alloxan-induced diabetic rabbits
title_sort practical considerations for reducing mortality rates in alloxan-induced diabetic rabbits
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7305394/
https://www.ncbi.nlm.nih.gov/pubmed/32577551
http://dx.doi.org/10.1016/j.heliyon.2020.e04103
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