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Amino acid metabolism, lipid metabolism, and oxidative stress are associated with post-stroke depression: a metabonomics study

BACKGROUND: Post-stroke depression (PSD) is a mood disorder characterized by depression and anhedonia caused by stroke. Metabolomics identified metabolites associated with PSD, but previous studies are based on gas chromatography (GC)/mass spectrometry (MS). This study aimed to perform a liquid chro...

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Autores principales: Wang, Man, Gui, Xianwei, Wu, Lanxiang, Tian, Sheng, Wang, Hansen, Xie, Liang, Wu, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7305607/
https://www.ncbi.nlm.nih.gov/pubmed/32563250
http://dx.doi.org/10.1186/s12883-020-01780-7
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author Wang, Man
Gui, Xianwei
Wu, Lanxiang
Tian, Sheng
Wang, Hansen
Xie, Liang
Wu, Wei
author_facet Wang, Man
Gui, Xianwei
Wu, Lanxiang
Tian, Sheng
Wang, Hansen
Xie, Liang
Wu, Wei
author_sort Wang, Man
collection PubMed
description BACKGROUND: Post-stroke depression (PSD) is a mood disorder characterized by depression and anhedonia caused by stroke. Metabolomics identified metabolites associated with PSD, but previous studies are based on gas chromatography (GC)/mass spectrometry (MS). This study aimed to perform a liquid chromatography (LC)-MS-based metabolomics study of the plasma metabolite profiles between patients with PSD and controls. METHODS: This was a prospective study of patients with stroke enrolled between July and December 2017 at the Second Affiliated Hospital of Nanchang University. Patients were grouped as Hamilton Depression Rating Scale > 7 (PSD) or < 7 (controls). Metabonomics profiling of plasma sampled was conducted by LC-MS. By combining multivariable and univariable statistical analyses, significant differential metabolites between the two groups were screened. The threshold for significant differences was VIP ≥1 and P < 0.05. Log(2)FC is the logarithm of the mean ratio between the two groups. RESULTS: There were no significant difference with respect to age, NIHSS score, and MMSE between the two groups (all P > 0.05). There were six differential metabolites between the PSD and stroke groups, of which three metabolites were increased and three were decreased. Compared with the control group, p-chlorophenylalanine (Log(2)FC = 1.37, P = 0.03), phenylacetyl glutamine (Log(2)FC = 0.21, P = 0.048), and DHA (Log(2)FC = 0.77, P = 0.01) levels were higher in the PSD group, while betaine (trimethylglycine) (Log(2)FC = − 0.79, P = 0.04), palmitic acid (Log(2)FC = − 0.51, P = 0.001), and MHPG-SO(4) (Log(2)FC = − 2.37, P = 0.045) were decreased. CONCLUSION: Plasma metabolomics showed that amino acid metabolism (phenylacetyl glutamine, p-chlorophenylalanine, trimethylglycine), lipid metabolism (DHA, palmitic acid, trimethylglycine), and oxidative stress (DHA, palmitic acid, trimethylglycine) were associated with PSD. These results could help to reveal the pathophysiological mechanism of PSD and eventually identify treatment targets.
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spelling pubmed-73056072020-06-22 Amino acid metabolism, lipid metabolism, and oxidative stress are associated with post-stroke depression: a metabonomics study Wang, Man Gui, Xianwei Wu, Lanxiang Tian, Sheng Wang, Hansen Xie, Liang Wu, Wei BMC Neurol Research Article BACKGROUND: Post-stroke depression (PSD) is a mood disorder characterized by depression and anhedonia caused by stroke. Metabolomics identified metabolites associated with PSD, but previous studies are based on gas chromatography (GC)/mass spectrometry (MS). This study aimed to perform a liquid chromatography (LC)-MS-based metabolomics study of the plasma metabolite profiles between patients with PSD and controls. METHODS: This was a prospective study of patients with stroke enrolled between July and December 2017 at the Second Affiliated Hospital of Nanchang University. Patients were grouped as Hamilton Depression Rating Scale > 7 (PSD) or < 7 (controls). Metabonomics profiling of plasma sampled was conducted by LC-MS. By combining multivariable and univariable statistical analyses, significant differential metabolites between the two groups were screened. The threshold for significant differences was VIP ≥1 and P < 0.05. Log(2)FC is the logarithm of the mean ratio between the two groups. RESULTS: There were no significant difference with respect to age, NIHSS score, and MMSE between the two groups (all P > 0.05). There were six differential metabolites between the PSD and stroke groups, of which three metabolites were increased and three were decreased. Compared with the control group, p-chlorophenylalanine (Log(2)FC = 1.37, P = 0.03), phenylacetyl glutamine (Log(2)FC = 0.21, P = 0.048), and DHA (Log(2)FC = 0.77, P = 0.01) levels were higher in the PSD group, while betaine (trimethylglycine) (Log(2)FC = − 0.79, P = 0.04), palmitic acid (Log(2)FC = − 0.51, P = 0.001), and MHPG-SO(4) (Log(2)FC = − 2.37, P = 0.045) were decreased. CONCLUSION: Plasma metabolomics showed that amino acid metabolism (phenylacetyl glutamine, p-chlorophenylalanine, trimethylglycine), lipid metabolism (DHA, palmitic acid, trimethylglycine), and oxidative stress (DHA, palmitic acid, trimethylglycine) were associated with PSD. These results could help to reveal the pathophysiological mechanism of PSD and eventually identify treatment targets. BioMed Central 2020-06-20 /pmc/articles/PMC7305607/ /pubmed/32563250 http://dx.doi.org/10.1186/s12883-020-01780-7 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Wang, Man
Gui, Xianwei
Wu, Lanxiang
Tian, Sheng
Wang, Hansen
Xie, Liang
Wu, Wei
Amino acid metabolism, lipid metabolism, and oxidative stress are associated with post-stroke depression: a metabonomics study
title Amino acid metabolism, lipid metabolism, and oxidative stress are associated with post-stroke depression: a metabonomics study
title_full Amino acid metabolism, lipid metabolism, and oxidative stress are associated with post-stroke depression: a metabonomics study
title_fullStr Amino acid metabolism, lipid metabolism, and oxidative stress are associated with post-stroke depression: a metabonomics study
title_full_unstemmed Amino acid metabolism, lipid metabolism, and oxidative stress are associated with post-stroke depression: a metabonomics study
title_short Amino acid metabolism, lipid metabolism, and oxidative stress are associated with post-stroke depression: a metabonomics study
title_sort amino acid metabolism, lipid metabolism, and oxidative stress are associated with post-stroke depression: a metabonomics study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7305607/
https://www.ncbi.nlm.nih.gov/pubmed/32563250
http://dx.doi.org/10.1186/s12883-020-01780-7
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