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A novel Cas9-targeted long-read assay for simultaneous detection of IDH1/2 mutations and clinically relevant MGMT methylation in fresh biopsies of diffuse glioma

Molecular biomarkers provide both diagnostic and prognostic results for patients with diffuse glioma, the most common primary brain tumor in adults. Here, we used a long-read nanopore-based sequencing technique to simultaneously assess IDH mutation status and MGMT methylation level in 4 human cell l...

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Detalles Bibliográficos
Autores principales:	Wongsurawat, Thidathip, Jenjaroenpun, Piroon, De Loose, Annick, Alkam, Duah, Ussery, David W., Nookaew, Intawat, Leung, Yuet-Kin, Ho, Shuk-Mei, Day, John D., Rodriguez, Analiz
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2020
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7305623/ https://www.ncbi.nlm.nih.gov/pubmed/32563269 http://dx.doi.org/10.1186/s40478-020-00963-0

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