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Epidermal growth factor in sacran hydrogel film accelerates fibroblast migration

Epidermal growth factor (EGF) accelerates epidermal regeneration, and it is widely studied as a wound-healing agent. However, the special carrier for the topical administration of EGF is urgently needed to deliver EGF on the wound site. In a preceding study, sacran hydrogel film (Sac-HF) showed a po...

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Autores principales: Wathoni, Nasrul, Rusdiana, Taofik, Hasanah, Aliya Nur, Pratama, Arvenda Rezky, Okajima, Maiko, Kaneko, Tatsuo, Mohammed, Ahmed Fouad Abdelwahab, Putera, Bayu Winata, Arima, Hidetoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7305779/
https://www.ncbi.nlm.nih.gov/pubmed/32587820
http://dx.doi.org/10.4103/japtr.JAPTR_147_19
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author Wathoni, Nasrul
Rusdiana, Taofik
Hasanah, Aliya Nur
Pratama, Arvenda Rezky
Okajima, Maiko
Kaneko, Tatsuo
Mohammed, Ahmed Fouad Abdelwahab
Putera, Bayu Winata
Arima, Hidetoshi
author_facet Wathoni, Nasrul
Rusdiana, Taofik
Hasanah, Aliya Nur
Pratama, Arvenda Rezky
Okajima, Maiko
Kaneko, Tatsuo
Mohammed, Ahmed Fouad Abdelwahab
Putera, Bayu Winata
Arima, Hidetoshi
author_sort Wathoni, Nasrul
collection PubMed
description Epidermal growth factor (EGF) accelerates epidermal regeneration, and it is widely studied as a wound-healing agent. However, the special carrier for the topical administration of EGF is urgently needed to deliver EGF on the wound site. In a preceding study, sacran hydrogel film (Sac-HF) showed a possible use as a dressing material for wound healing, as well as a good capability as a drug carrier. In the current study, we prepared Sac-HF containing EGF (Sac/EGF-HF) and then characterized their physicochemical properties, including thickness, swelling ratio, degradability, tensile strength, and morphology. In addition, we have also conducted thermal and crystallography studies using differential scanning calorimetry (DSC) and X-ray diffraction, respectively. Furthermore, we investigated the in vitro influence of Sac/EGF-HF on cell migration using a fibroblast cell line. Morphology study confirmed that the casting method used for the film preparation resulted in a homogeneous film of Sac/EGF-HF. Furthermore, EGF significantly increased the thickness, tensile strength, and degradability of Sac/EGF-HF compared to Sac-HF. Sac/EGF-HF had a lower swelling ability compared to Sac-HF; this result corroborated the tensile strength result. Interestingly, X-ray diffraction and DSC results showed that Sac/EGF-HF had an amorphous shape. The in vitro studies revealed that Sac/EGF-HF induced the fibroblast migration activity. These results conclude that Sac/EGF-HF has the potential properties of HF for biomedical applications.
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spelling pubmed-73057792020-06-24 Epidermal growth factor in sacran hydrogel film accelerates fibroblast migration Wathoni, Nasrul Rusdiana, Taofik Hasanah, Aliya Nur Pratama, Arvenda Rezky Okajima, Maiko Kaneko, Tatsuo Mohammed, Ahmed Fouad Abdelwahab Putera, Bayu Winata Arima, Hidetoshi J Adv Pharm Technol Res Original Article Epidermal growth factor (EGF) accelerates epidermal regeneration, and it is widely studied as a wound-healing agent. However, the special carrier for the topical administration of EGF is urgently needed to deliver EGF on the wound site. In a preceding study, sacran hydrogel film (Sac-HF) showed a possible use as a dressing material for wound healing, as well as a good capability as a drug carrier. In the current study, we prepared Sac-HF containing EGF (Sac/EGF-HF) and then characterized their physicochemical properties, including thickness, swelling ratio, degradability, tensile strength, and morphology. In addition, we have also conducted thermal and crystallography studies using differential scanning calorimetry (DSC) and X-ray diffraction, respectively. Furthermore, we investigated the in vitro influence of Sac/EGF-HF on cell migration using a fibroblast cell line. Morphology study confirmed that the casting method used for the film preparation resulted in a homogeneous film of Sac/EGF-HF. Furthermore, EGF significantly increased the thickness, tensile strength, and degradability of Sac/EGF-HF compared to Sac-HF. Sac/EGF-HF had a lower swelling ability compared to Sac-HF; this result corroborated the tensile strength result. Interestingly, X-ray diffraction and DSC results showed that Sac/EGF-HF had an amorphous shape. The in vitro studies revealed that Sac/EGF-HF induced the fibroblast migration activity. These results conclude that Sac/EGF-HF has the potential properties of HF for biomedical applications. Wolters Kluwer - Medknow 2020 2020-04-22 /pmc/articles/PMC7305779/ /pubmed/32587820 http://dx.doi.org/10.4103/japtr.JAPTR_147_19 Text en Copyright: © 2020 Journal of Advanced Pharmaceutical Technology & Research http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Wathoni, Nasrul
Rusdiana, Taofik
Hasanah, Aliya Nur
Pratama, Arvenda Rezky
Okajima, Maiko
Kaneko, Tatsuo
Mohammed, Ahmed Fouad Abdelwahab
Putera, Bayu Winata
Arima, Hidetoshi
Epidermal growth factor in sacran hydrogel film accelerates fibroblast migration
title Epidermal growth factor in sacran hydrogel film accelerates fibroblast migration
title_full Epidermal growth factor in sacran hydrogel film accelerates fibroblast migration
title_fullStr Epidermal growth factor in sacran hydrogel film accelerates fibroblast migration
title_full_unstemmed Epidermal growth factor in sacran hydrogel film accelerates fibroblast migration
title_short Epidermal growth factor in sacran hydrogel film accelerates fibroblast migration
title_sort epidermal growth factor in sacran hydrogel film accelerates fibroblast migration
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7305779/
https://www.ncbi.nlm.nih.gov/pubmed/32587820
http://dx.doi.org/10.4103/japtr.JAPTR_147_19
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