Cargando…
Real-time monitoring of rhamnose induction effect on the expression of mpt64 gene fused with pelB signal peptide in Escherichia coli BL21 (DE3)
In this research, Escherichia coli BL21 (DE3) harboring an expression vector constructed with a rhamnose-inducible promoter and a pelB signal peptide was used as a host cell to produce MPT64 protein. The objective of this research was to figure out the optimum time of mpt64 gene expression through r...
Autores principales: | Kusuma, Sri Agung Fitri, Parwati, Ida, Subroto, Toto, Rukayadi, Yaya, Rostinawati, Tina, Yusuf, Muhammad, Fadhlillah, Muhammad, Tanti, Laily D., Ahyudanari, Risa R. |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7305781/ https://www.ncbi.nlm.nih.gov/pubmed/32587819 http://dx.doi.org/10.4103/japtr.JAPTR_120_19 |
Ejemplares similares
-
Improving of pelB-Secreted MPT64 protein released by Escherichia coli BL21 (DE3) using Triton X-100 and Tween-80
por: Kusuma, Sri Agung Fitri, et al.
Publicado: (2022) -
Optimization of culture conditions for Mpt64 synthetic gene expression in Escherichia coli BL21 (DE3) using surface response methodology
por: Kusuma, Sri Agung Fitri, et al.
Publicado: (2019) -
Gel Protein Extraction’s Impact on Conformational Epitopes of Linear Non-Tagged MPT64 Protein
por: Kusuma, Sri Agung Fitri, et al.
Publicado: (2023) -
Comparison of simple and rapid extracting methods of free-tags Mycobacterium tuberculosis protein 64 Recombinant Protein from polyacrylamide gel: Electroelution and the optimized passive elution
por: Kusuma, Sri Agung Fitri, et al.
Publicado: (2021) -
Synonymous Codons
and Hydrophobicity Optimization
of Post-translational Signal Peptide PelB Increase Phage Display Efficiency
of DARPins
por: Kulmala, Antti, et al.
Publicado: (2022)