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Rational design of a new mutant of tobacco etch virus protease in order to increase the in vitro solubility

BACKGROUND AND PURPOSE: Tobacco etch virus (TEV) protease is a protease with high sequence specificity which is useful for the cleavage of fusion proteins. A major limitation of this enzyme is its relatively poor solubility. This study aimed to investigate the effects of some suggested mutations by...

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Detalles Bibliográficos
Autores principales: Mohammadian, Hossein, Mahnam, Karim, Sadeghi, Hamid Mirmohammad, Ganjalikhany, Mohamad Reza, Akbari, Vajihe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7306250/
https://www.ncbi.nlm.nih.gov/pubmed/32582356
http://dx.doi.org/10.4103/1735-5362.283816