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Structural and catalytic effects of surface loop-helix transplantation within haloalkane dehalogenase family
Engineering enzyme catalytic properties is important for basic research as well as for biotechnological applications. We have previously shown that the reshaping of enzyme access tunnels via the deletion of a short surface loop element may yield a haloalkane dehalogenase variant with markedly modifi...
Autores principales: | Marek, Martin, Chaloupkova, Radka, Prudnikova, Tatyana, Sato, Yukari, Rezacova, Pavlina, Nagata, Yuji, Kuta Smatanova, Ivana, Damborsky, Jiri |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Research Network of Computational and Structural Biotechnology
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7306515/ https://www.ncbi.nlm.nih.gov/pubmed/32612758 http://dx.doi.org/10.1016/j.csbj.2020.05.019 |
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