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Application of serine integrases for secondary metabolite pathway assembly in Streptomyces
Serine integrases have been shown to be efficient tools for metabolic pathway assembly. To further improve the flexibility and efficiency of pathway engineering via serine integrases, we explored how multiple orthogonally active serine integrases can be applied for use in vitro for the heterologous...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
KeAi Publishing
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7306541/ https://www.ncbi.nlm.nih.gov/pubmed/32596521 http://dx.doi.org/10.1016/j.synbio.2020.05.006 |
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author | Gao, Hong Taylor, Gabrielle Evans, Stephanie K. Fogg, Paul C.M. Smith, Margaret C.M. |
author_facet | Gao, Hong Taylor, Gabrielle Evans, Stephanie K. Fogg, Paul C.M. Smith, Margaret C.M. |
author_sort | Gao, Hong |
collection | PubMed |
description | Serine integrases have been shown to be efficient tools for metabolic pathway assembly. To further improve the flexibility and efficiency of pathway engineering via serine integrases, we explored how multiple orthogonally active serine integrases can be applied for use in vitro for the heterologous expression of complex biosynthesis pathways in Streptomyces spp., the major producers of useful bioactive natural products. The results show that multiple orthogonal serine integrases efficiently assemble the genes from a complex biosynthesis pathway in a single in vitro recombination reaction, potentially permitting a versatile combinatorial assembly approach. Furthermore, the assembly strategy also permitted the incorporation of a well-characterised promoter upstream of each gene for expression in a heterologous host. The results demonstrate how site-specific recombination based on orthogonal serine integrases can be applied in Streptomyces spp. |
format | Online Article Text |
id | pubmed-7306541 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | KeAi Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-73065412020-06-25 Application of serine integrases for secondary metabolite pathway assembly in Streptomyces Gao, Hong Taylor, Gabrielle Evans, Stephanie K. Fogg, Paul C.M. Smith, Margaret C.M. Synth Syst Biotechnol Article Serine integrases have been shown to be efficient tools for metabolic pathway assembly. To further improve the flexibility and efficiency of pathway engineering via serine integrases, we explored how multiple orthogonally active serine integrases can be applied for use in vitro for the heterologous expression of complex biosynthesis pathways in Streptomyces spp., the major producers of useful bioactive natural products. The results show that multiple orthogonal serine integrases efficiently assemble the genes from a complex biosynthesis pathway in a single in vitro recombination reaction, potentially permitting a versatile combinatorial assembly approach. Furthermore, the assembly strategy also permitted the incorporation of a well-characterised promoter upstream of each gene for expression in a heterologous host. The results demonstrate how site-specific recombination based on orthogonal serine integrases can be applied in Streptomyces spp. KeAi Publishing 2020-06-18 /pmc/articles/PMC7306541/ /pubmed/32596521 http://dx.doi.org/10.1016/j.synbio.2020.05.006 Text en © 2020 KeAi Communications Co.(+) Ltd http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Gao, Hong Taylor, Gabrielle Evans, Stephanie K. Fogg, Paul C.M. Smith, Margaret C.M. Application of serine integrases for secondary metabolite pathway assembly in Streptomyces |
title | Application of serine integrases for secondary metabolite pathway assembly in Streptomyces |
title_full | Application of serine integrases for secondary metabolite pathway assembly in Streptomyces |
title_fullStr | Application of serine integrases for secondary metabolite pathway assembly in Streptomyces |
title_full_unstemmed | Application of serine integrases for secondary metabolite pathway assembly in Streptomyces |
title_short | Application of serine integrases for secondary metabolite pathway assembly in Streptomyces |
title_sort | application of serine integrases for secondary metabolite pathway assembly in streptomyces |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7306541/ https://www.ncbi.nlm.nih.gov/pubmed/32596521 http://dx.doi.org/10.1016/j.synbio.2020.05.006 |
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