Cargando…
The Putative Methyltransferase TlLAE1 Is Involved in the Regulation of Peptaibols Production in the Biocontrol Fungus Trichoderma longibrachiatum SMF2
The biocontrol fungus Trichoderma longibrachiatum SMF2 secretes a large quantity of peptaibols that have been shown to have a range of biological activities and therefore great application values. However, the mechanism of the regulatory expression of peptaibols is still unclear. The putative methyl...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7307461/ https://www.ncbi.nlm.nih.gov/pubmed/32612590 http://dx.doi.org/10.3389/fmicb.2020.01267 |
_version_ | 1783548812806062080 |
---|---|
author | Shi, Jin-Chao Shi, Wei-Ling Zhou, Yan-Rong Chen, Xiu-Lan Zhang, Yu-Zhong Zhang, Xia Zhang, Wei-Xin Song, Xiao-Yan |
author_facet | Shi, Jin-Chao Shi, Wei-Ling Zhou, Yan-Rong Chen, Xiu-Lan Zhang, Yu-Zhong Zhang, Xia Zhang, Wei-Xin Song, Xiao-Yan |
author_sort | Shi, Jin-Chao |
collection | PubMed |
description | The biocontrol fungus Trichoderma longibrachiatum SMF2 secretes a large quantity of peptaibols that have been shown to have a range of biological activities and therefore great application values. However, the mechanism of the regulatory expression of peptaibols is still unclear. The putative methyltransferase LaeA/LAE1 is a global regulator involved in the biosynthesis of some secondary metabolites in filamentous fungi. In this study, we demonstrated that the ortholog of LaeA/LAE1 in the biocontrol fungus T. longibrachiatum SMF2, TlLAE1, plays an important role in the regulation of peptaibols production. Deletion of Tllae1 resulted in a slight negative impact on mycelial growth, and a significant defect in conidial production. Deletion of Tllae1 also compromised the production of peptaibols to a large degree. Further analyses indicated that this defect occurred at the transcriptional level of the two synthetases-encoding genes, tlx1 and tlx2, which are responsible for peptaibols production. By contrast, constitutive expression of Tllae1 in T. longibrachiatum SMF2 led to 2-fold increased peptaibols production, suggesting that this is a strategy to improve peptaibols production in Trichoderma fungi. These results demonstrate the important role of LAE1 in the regulation of peptaibols production in T. longibrachiatum SMF2. |
format | Online Article Text |
id | pubmed-7307461 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-73074612020-06-30 The Putative Methyltransferase TlLAE1 Is Involved in the Regulation of Peptaibols Production in the Biocontrol Fungus Trichoderma longibrachiatum SMF2 Shi, Jin-Chao Shi, Wei-Ling Zhou, Yan-Rong Chen, Xiu-Lan Zhang, Yu-Zhong Zhang, Xia Zhang, Wei-Xin Song, Xiao-Yan Front Microbiol Microbiology The biocontrol fungus Trichoderma longibrachiatum SMF2 secretes a large quantity of peptaibols that have been shown to have a range of biological activities and therefore great application values. However, the mechanism of the regulatory expression of peptaibols is still unclear. The putative methyltransferase LaeA/LAE1 is a global regulator involved in the biosynthesis of some secondary metabolites in filamentous fungi. In this study, we demonstrated that the ortholog of LaeA/LAE1 in the biocontrol fungus T. longibrachiatum SMF2, TlLAE1, plays an important role in the regulation of peptaibols production. Deletion of Tllae1 resulted in a slight negative impact on mycelial growth, and a significant defect in conidial production. Deletion of Tllae1 also compromised the production of peptaibols to a large degree. Further analyses indicated that this defect occurred at the transcriptional level of the two synthetases-encoding genes, tlx1 and tlx2, which are responsible for peptaibols production. By contrast, constitutive expression of Tllae1 in T. longibrachiatum SMF2 led to 2-fold increased peptaibols production, suggesting that this is a strategy to improve peptaibols production in Trichoderma fungi. These results demonstrate the important role of LAE1 in the regulation of peptaibols production in T. longibrachiatum SMF2. Frontiers Media S.A. 2020-06-12 /pmc/articles/PMC7307461/ /pubmed/32612590 http://dx.doi.org/10.3389/fmicb.2020.01267 Text en Copyright © 2020 Shi, Shi, Zhou, Chen, Zhang, Zhang, Zhang and Song. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Shi, Jin-Chao Shi, Wei-Ling Zhou, Yan-Rong Chen, Xiu-Lan Zhang, Yu-Zhong Zhang, Xia Zhang, Wei-Xin Song, Xiao-Yan The Putative Methyltransferase TlLAE1 Is Involved in the Regulation of Peptaibols Production in the Biocontrol Fungus Trichoderma longibrachiatum SMF2 |
title | The Putative Methyltransferase TlLAE1 Is Involved in the Regulation of Peptaibols Production in the Biocontrol Fungus Trichoderma longibrachiatum SMF2 |
title_full | The Putative Methyltransferase TlLAE1 Is Involved in the Regulation of Peptaibols Production in the Biocontrol Fungus Trichoderma longibrachiatum SMF2 |
title_fullStr | The Putative Methyltransferase TlLAE1 Is Involved in the Regulation of Peptaibols Production in the Biocontrol Fungus Trichoderma longibrachiatum SMF2 |
title_full_unstemmed | The Putative Methyltransferase TlLAE1 Is Involved in the Regulation of Peptaibols Production in the Biocontrol Fungus Trichoderma longibrachiatum SMF2 |
title_short | The Putative Methyltransferase TlLAE1 Is Involved in the Regulation of Peptaibols Production in the Biocontrol Fungus Trichoderma longibrachiatum SMF2 |
title_sort | putative methyltransferase tllae1 is involved in the regulation of peptaibols production in the biocontrol fungus trichoderma longibrachiatum smf2 |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7307461/ https://www.ncbi.nlm.nih.gov/pubmed/32612590 http://dx.doi.org/10.3389/fmicb.2020.01267 |
work_keys_str_mv | AT shijinchao theputativemethyltransferasetllae1isinvolvedintheregulationofpeptaibolsproductioninthebiocontrolfungustrichodermalongibrachiatumsmf2 AT shiweiling theputativemethyltransferasetllae1isinvolvedintheregulationofpeptaibolsproductioninthebiocontrolfungustrichodermalongibrachiatumsmf2 AT zhouyanrong theputativemethyltransferasetllae1isinvolvedintheregulationofpeptaibolsproductioninthebiocontrolfungustrichodermalongibrachiatumsmf2 AT chenxiulan theputativemethyltransferasetllae1isinvolvedintheregulationofpeptaibolsproductioninthebiocontrolfungustrichodermalongibrachiatumsmf2 AT zhangyuzhong theputativemethyltransferasetllae1isinvolvedintheregulationofpeptaibolsproductioninthebiocontrolfungustrichodermalongibrachiatumsmf2 AT zhangxia theputativemethyltransferasetllae1isinvolvedintheregulationofpeptaibolsproductioninthebiocontrolfungustrichodermalongibrachiatumsmf2 AT zhangweixin theputativemethyltransferasetllae1isinvolvedintheregulationofpeptaibolsproductioninthebiocontrolfungustrichodermalongibrachiatumsmf2 AT songxiaoyan theputativemethyltransferasetllae1isinvolvedintheregulationofpeptaibolsproductioninthebiocontrolfungustrichodermalongibrachiatumsmf2 AT shijinchao putativemethyltransferasetllae1isinvolvedintheregulationofpeptaibolsproductioninthebiocontrolfungustrichodermalongibrachiatumsmf2 AT shiweiling putativemethyltransferasetllae1isinvolvedintheregulationofpeptaibolsproductioninthebiocontrolfungustrichodermalongibrachiatumsmf2 AT zhouyanrong putativemethyltransferasetllae1isinvolvedintheregulationofpeptaibolsproductioninthebiocontrolfungustrichodermalongibrachiatumsmf2 AT chenxiulan putativemethyltransferasetllae1isinvolvedintheregulationofpeptaibolsproductioninthebiocontrolfungustrichodermalongibrachiatumsmf2 AT zhangyuzhong putativemethyltransferasetllae1isinvolvedintheregulationofpeptaibolsproductioninthebiocontrolfungustrichodermalongibrachiatumsmf2 AT zhangxia putativemethyltransferasetllae1isinvolvedintheregulationofpeptaibolsproductioninthebiocontrolfungustrichodermalongibrachiatumsmf2 AT zhangweixin putativemethyltransferasetllae1isinvolvedintheregulationofpeptaibolsproductioninthebiocontrolfungustrichodermalongibrachiatumsmf2 AT songxiaoyan putativemethyltransferasetllae1isinvolvedintheregulationofpeptaibolsproductioninthebiocontrolfungustrichodermalongibrachiatumsmf2 |