Cargando…
An Open Source Automated Bar Test for Measuring Catalepsy in Rats
Catalepsy bar tests are widely used to measure the failure to correct an imposed posture resulting from muscular rigidity. Procedures for measuring catalepsy vary greatly in the published literature, but one commonly used test measures the time it takes for a rodent to remove one or both of its fore...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Society for Neuroscience
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7307632/ https://www.ncbi.nlm.nih.gov/pubmed/32198157 http://dx.doi.org/10.1523/ENEURO.0488-19.2020 |
_version_ | 1783548844960645120 |
---|---|
author | Luciani, Karling R. Frie, Jude A. Khokhar, Jibran Y. |
author_facet | Luciani, Karling R. Frie, Jude A. Khokhar, Jibran Y. |
author_sort | Luciani, Karling R. |
collection | PubMed |
description | Catalepsy bar tests are widely used to measure the failure to correct an imposed posture resulting from muscular rigidity. Procedures for measuring catalepsy vary greatly in the published literature, but one commonly used test measures the time it takes for a rodent to remove one or both of its forelimbs from a bar. The following paper describes an affordable, adjustable, open-source bar test that automatically measures and logs the time it takes for a rat to remove itself from a bar. While commercially available automated bar tests are prohibitively expensive, requiring proprietary software and hardware to operate, the proposed apparatus runs on an Arduino-based microcontroller making it low-cost and customizable. This 3D-printed design costs less than 65 United States dollars to build and is simple to assemble and operate. The beam-break sensor design also eliminates many of the pitfalls of the “complete-the-circuit”-based approach to recording catalepsy. The paper further describes the successful validation of the design using adult male rats injected with different doses of haloperidol to demonstrate a dose-dependent cataleptic effect. This design provides a versatile, low-cost solution to standardizing and automating measurement of catalepsy in rodents. |
format | Online Article Text |
id | pubmed-7307632 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Society for Neuroscience |
record_format | MEDLINE/PubMed |
spelling | pubmed-73076322020-06-23 An Open Source Automated Bar Test for Measuring Catalepsy in Rats Luciani, Karling R. Frie, Jude A. Khokhar, Jibran Y. eNeuro Open Source Tools and Methods Catalepsy bar tests are widely used to measure the failure to correct an imposed posture resulting from muscular rigidity. Procedures for measuring catalepsy vary greatly in the published literature, but one commonly used test measures the time it takes for a rodent to remove one or both of its forelimbs from a bar. The following paper describes an affordable, adjustable, open-source bar test that automatically measures and logs the time it takes for a rat to remove itself from a bar. While commercially available automated bar tests are prohibitively expensive, requiring proprietary software and hardware to operate, the proposed apparatus runs on an Arduino-based microcontroller making it low-cost and customizable. This 3D-printed design costs less than 65 United States dollars to build and is simple to assemble and operate. The beam-break sensor design also eliminates many of the pitfalls of the “complete-the-circuit”-based approach to recording catalepsy. The paper further describes the successful validation of the design using adult male rats injected with different doses of haloperidol to demonstrate a dose-dependent cataleptic effect. This design provides a versatile, low-cost solution to standardizing and automating measurement of catalepsy in rodents. Society for Neuroscience 2020-06-19 /pmc/articles/PMC7307632/ /pubmed/32198157 http://dx.doi.org/10.1523/ENEURO.0488-19.2020 Text en Copyright © 2020 Luciani et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Open Source Tools and Methods Luciani, Karling R. Frie, Jude A. Khokhar, Jibran Y. An Open Source Automated Bar Test for Measuring Catalepsy in Rats |
title | An Open Source Automated Bar Test for Measuring Catalepsy in Rats |
title_full | An Open Source Automated Bar Test for Measuring Catalepsy in Rats |
title_fullStr | An Open Source Automated Bar Test for Measuring Catalepsy in Rats |
title_full_unstemmed | An Open Source Automated Bar Test for Measuring Catalepsy in Rats |
title_short | An Open Source Automated Bar Test for Measuring Catalepsy in Rats |
title_sort | open source automated bar test for measuring catalepsy in rats |
topic | Open Source Tools and Methods |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7307632/ https://www.ncbi.nlm.nih.gov/pubmed/32198157 http://dx.doi.org/10.1523/ENEURO.0488-19.2020 |
work_keys_str_mv | AT lucianikarlingr anopensourceautomatedbartestformeasuringcatalepsyinrats AT friejudea anopensourceautomatedbartestformeasuringcatalepsyinrats AT khokharjibrany anopensourceautomatedbartestformeasuringcatalepsyinrats AT lucianikarlingr opensourceautomatedbartestformeasuringcatalepsyinrats AT friejudea opensourceautomatedbartestformeasuringcatalepsyinrats AT khokharjibrany opensourceautomatedbartestformeasuringcatalepsyinrats |