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Isolation of cancer-derived extracellular vesicle subpopulations by a size-selective microfluidic platform

Extracellular vesicles (EVs) play an important role in intercellular communication. Recently, there has been increasing interest in EVs as potential diagnostic biomarkers and therapeutic vehicles. However, the molecular properties and cargo information of EV subpopulations have not yet been fully in...

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Autores principales: Chen, Zheyuan, Yang, Yi, Yamaguchi, Hirohito, Hung, Mien-Chi, Kameoka, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AIP Publishing LLC 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7307647/
https://www.ncbi.nlm.nih.gov/pubmed/32577148
http://dx.doi.org/10.1063/5.0008438
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author Chen, Zheyuan
Yang, Yi
Yamaguchi, Hirohito
Hung, Mien-Chi
Kameoka, Jun
author_facet Chen, Zheyuan
Yang, Yi
Yamaguchi, Hirohito
Hung, Mien-Chi
Kameoka, Jun
author_sort Chen, Zheyuan
collection PubMed
description Extracellular vesicles (EVs) play an important role in intercellular communication. Recently, there has been increasing interest in EVs as potential diagnostic biomarkers and therapeutic vehicles. However, the molecular properties and cargo information of EV subpopulations have not yet been fully investigated due to lack of reliable and reproducible EV separation technology. Current approaches have faced difficulties with efficiently isolating EVs from biofluids, especially subpopulations of small EVs. Here, we report an EV isolation method based on a size-selective microfluidic platform (ExoSMP) via nanomembrane filtration and electrophoretic force. This unique platform offers an enhanced approach to sorting a heterogeneous population of EVs based on size, with the additional advantages of being label-free and low-cost, and featuring a short processing time (<1 h), and convenient integration with downstream analysis. In this research, we used ExoSMP to demonstrate the isolation of cancer-derived small EVs (30–120 nm) with high recovery (94.2%) and reproducibility at an optimum sample flow rate. Furthermore, we investigated isolation of EV subpopulations by altering nanomembrane combinations with different pore size combinations (50 and 100 nm, 30 and 100 nm, 30 and 200 nm, and 30 and 50 nm). This ExoSMP technique can serve as a standardized EV isolation/separation tool, facilitating the clinical prospects of EVs and opening up a new avenue for future point-of-care applications in liquid biopsies.
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spelling pubmed-73076472020-06-22 Isolation of cancer-derived extracellular vesicle subpopulations by a size-selective microfluidic platform Chen, Zheyuan Yang, Yi Yamaguchi, Hirohito Hung, Mien-Chi Kameoka, Jun Biomicrofluidics Regular Articles Extracellular vesicles (EVs) play an important role in intercellular communication. Recently, there has been increasing interest in EVs as potential diagnostic biomarkers and therapeutic vehicles. However, the molecular properties and cargo information of EV subpopulations have not yet been fully investigated due to lack of reliable and reproducible EV separation technology. Current approaches have faced difficulties with efficiently isolating EVs from biofluids, especially subpopulations of small EVs. Here, we report an EV isolation method based on a size-selective microfluidic platform (ExoSMP) via nanomembrane filtration and electrophoretic force. This unique platform offers an enhanced approach to sorting a heterogeneous population of EVs based on size, with the additional advantages of being label-free and low-cost, and featuring a short processing time (<1 h), and convenient integration with downstream analysis. In this research, we used ExoSMP to demonstrate the isolation of cancer-derived small EVs (30–120 nm) with high recovery (94.2%) and reproducibility at an optimum sample flow rate. Furthermore, we investigated isolation of EV subpopulations by altering nanomembrane combinations with different pore size combinations (50 and 100 nm, 30 and 100 nm, 30 and 200 nm, and 30 and 50 nm). This ExoSMP technique can serve as a standardized EV isolation/separation tool, facilitating the clinical prospects of EVs and opening up a new avenue for future point-of-care applications in liquid biopsies. AIP Publishing LLC 2020-06-08 /pmc/articles/PMC7307647/ /pubmed/32577148 http://dx.doi.org/10.1063/5.0008438 Text en Copyright © 2020 Author(s) Published under license by AIP Publishing. 1932-1058/2020/14(3)/034113/10/$30.00 All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Regular Articles
Chen, Zheyuan
Yang, Yi
Yamaguchi, Hirohito
Hung, Mien-Chi
Kameoka, Jun
Isolation of cancer-derived extracellular vesicle subpopulations by a size-selective microfluidic platform
title Isolation of cancer-derived extracellular vesicle subpopulations by a size-selective microfluidic platform
title_full Isolation of cancer-derived extracellular vesicle subpopulations by a size-selective microfluidic platform
title_fullStr Isolation of cancer-derived extracellular vesicle subpopulations by a size-selective microfluidic platform
title_full_unstemmed Isolation of cancer-derived extracellular vesicle subpopulations by a size-selective microfluidic platform
title_short Isolation of cancer-derived extracellular vesicle subpopulations by a size-selective microfluidic platform
title_sort isolation of cancer-derived extracellular vesicle subpopulations by a size-selective microfluidic platform
topic Regular Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7307647/
https://www.ncbi.nlm.nih.gov/pubmed/32577148
http://dx.doi.org/10.1063/5.0008438
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