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Low expression of microRNA-328 can predict sepsis and alleviate sepsis-induced cardiac dysfunction and inflammatory response
Sepsis often leads to cardiac dysfunction and inflammation. This study investigated the clinical value of microRNA-328 (miR-328) in sepsis and its role in cardiac dysfunction and inflammation caused by sepsis. The expression level of miR-328 in the serum of the subjects was detected by qRT-PCR. Rece...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Associação Brasileira de Divulgação Científica
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7307893/ https://www.ncbi.nlm.nih.gov/pubmed/32578720 http://dx.doi.org/10.1590/1414-431X20209501 |
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author | Sun, Bin Luan, Chunye Guo, Lisha Zhang, Bing Liu, Yufang |
author_facet | Sun, Bin Luan, Chunye Guo, Lisha Zhang, Bing Liu, Yufang |
author_sort | Sun, Bin |
collection | PubMed |
description | Sepsis often leads to cardiac dysfunction and inflammation. This study investigated the clinical value of microRNA-328 (miR-328) in sepsis and its role in cardiac dysfunction and inflammation caused by sepsis. The expression level of miR-328 in the serum of the subjects was detected by qRT-PCR. Receiver operating characteristic (ROC) curve measured the diagnostic value of miR-328 in sepsis. Rat sepsis model was established to detect left ventricular systolic pressure (LVSP), left ventricular end-diastolic pressure (LVEDP), and maximal rate of increase/decrease of left ventricular pressure (±dp/dt(max)). Myocardial injury markers serum cardiac troponin I (cTnI), myocardial kinase isoenzyme (CK-MB), and inflammatory factors were detected by enzyme-linked immunosorbent assay (ELISA). miR-328 expression was assessed in serum of sepsis patients and in rat models of sepsis. The AUC of ROC curve was 0.926, sensitivity 87.60%, and specificity 86.36%. Compared with the sham group, LVSP and +dp/dt(max) were decreased in the rat model of sepsis. LVEDP, -dp/dt(max), cTnI, CK-MB, tumor necrosis factor-α, interleukin (IL)-6, and IL-1β were upregulated in the rat model of sepsis. The low expression of miR-328 reversed these indicators. miR-328 is a diagnostic marker for patients with sepsis, and decreasing the expression level of miR-328 can ameliorate cardiac dysfunction and cardiac inflammation in sepsis. |
format | Online Article Text |
id | pubmed-7307893 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Associação Brasileira de Divulgação Científica |
record_format | MEDLINE/PubMed |
spelling | pubmed-73078932020-06-26 Low expression of microRNA-328 can predict sepsis and alleviate sepsis-induced cardiac dysfunction and inflammatory response Sun, Bin Luan, Chunye Guo, Lisha Zhang, Bing Liu, Yufang Braz J Med Biol Res Research Article Sepsis often leads to cardiac dysfunction and inflammation. This study investigated the clinical value of microRNA-328 (miR-328) in sepsis and its role in cardiac dysfunction and inflammation caused by sepsis. The expression level of miR-328 in the serum of the subjects was detected by qRT-PCR. Receiver operating characteristic (ROC) curve measured the diagnostic value of miR-328 in sepsis. Rat sepsis model was established to detect left ventricular systolic pressure (LVSP), left ventricular end-diastolic pressure (LVEDP), and maximal rate of increase/decrease of left ventricular pressure (±dp/dt(max)). Myocardial injury markers serum cardiac troponin I (cTnI), myocardial kinase isoenzyme (CK-MB), and inflammatory factors were detected by enzyme-linked immunosorbent assay (ELISA). miR-328 expression was assessed in serum of sepsis patients and in rat models of sepsis. The AUC of ROC curve was 0.926, sensitivity 87.60%, and specificity 86.36%. Compared with the sham group, LVSP and +dp/dt(max) were decreased in the rat model of sepsis. LVEDP, -dp/dt(max), cTnI, CK-MB, tumor necrosis factor-α, interleukin (IL)-6, and IL-1β were upregulated in the rat model of sepsis. The low expression of miR-328 reversed these indicators. miR-328 is a diagnostic marker for patients with sepsis, and decreasing the expression level of miR-328 can ameliorate cardiac dysfunction and cardiac inflammation in sepsis. Associação Brasileira de Divulgação Científica 2020-06-19 /pmc/articles/PMC7307893/ /pubmed/32578720 http://dx.doi.org/10.1590/1414-431X20209501 Text en https://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Sun, Bin Luan, Chunye Guo, Lisha Zhang, Bing Liu, Yufang Low expression of microRNA-328 can predict sepsis and alleviate sepsis-induced cardiac dysfunction and inflammatory response |
title | Low expression of microRNA-328 can predict sepsis and alleviate
sepsis-induced cardiac dysfunction and inflammatory response |
title_full | Low expression of microRNA-328 can predict sepsis and alleviate
sepsis-induced cardiac dysfunction and inflammatory response |
title_fullStr | Low expression of microRNA-328 can predict sepsis and alleviate
sepsis-induced cardiac dysfunction and inflammatory response |
title_full_unstemmed | Low expression of microRNA-328 can predict sepsis and alleviate
sepsis-induced cardiac dysfunction and inflammatory response |
title_short | Low expression of microRNA-328 can predict sepsis and alleviate
sepsis-induced cardiac dysfunction and inflammatory response |
title_sort | low expression of microrna-328 can predict sepsis and alleviate
sepsis-induced cardiac dysfunction and inflammatory response |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7307893/ https://www.ncbi.nlm.nih.gov/pubmed/32578720 http://dx.doi.org/10.1590/1414-431X20209501 |
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