Development of a cell-free split-luciferase biochemical assay as a tool for screening for inhibitors of challenging protein-protein interaction targets

Targeting the interaction of proteins with weak binding affinities or low solubility represents a particular challenge for drug screening. The NanoLuc (â )® Binary Technology (NanoBiT (â )®) was originally developed to detect protein-protein interactions in live mammalian cells. Here we report the s...

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Detalles Bibliográficos
Autores principales: Cooley, Rachel, Kara, Neesha, Hui, Ning Sze, Tart, Jonathan, Roustan, Chloë, George, Roger, Hancock, David C., Binkowski, Brock F., Wood, Keith V., Ismail, Mohamed, Downward, Julian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000 Research Limited 2020
Materias:
Method Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7308888/
https://www.ncbi.nlm.nih.gov/pubmed/32587898
http://dx.doi.org/10.12688/wellcomeopenres.15675.1

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