Cargando…
Repetitive Detection of Aromatic Hydrocarbon Contaminants with Bioluminescent Bioreporters Attached on Tapered Optical Fiber Elements
In this study, we show the repetitive detection of toluene on a tapered optical fiber element (OFE) with an attached layer of Pseudomonas putida TVA8 bioluminescent bioreporters. The bioluminescent cell layer was attached on polished quartz modified with (3-aminopropyl)triethoxysilane (APTES). The r...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7309017/ https://www.ncbi.nlm.nih.gov/pubmed/32517218 http://dx.doi.org/10.3390/s20113237 |
_version_ | 1783549126617595904 |
---|---|
author | Zajíc, Jakub Ripp, Steven Trögl, Josef Kuncová, Gabriela Pospíšilová, Marie |
author_facet | Zajíc, Jakub Ripp, Steven Trögl, Josef Kuncová, Gabriela Pospíšilová, Marie |
author_sort | Zajíc, Jakub |
collection | PubMed |
description | In this study, we show the repetitive detection of toluene on a tapered optical fiber element (OFE) with an attached layer of Pseudomonas putida TVA8 bioluminescent bioreporters. The bioluminescent cell layer was attached on polished quartz modified with (3-aminopropyl)triethoxysilane (APTES). The repeatability of the preparation of the optical probe and its use was demonstrated with five differently shaped OFEs. The intensity of measured bioluminescence was minimally influenced by the OFE shape, possessing transmittances between 1.41% and 5.00%. OFE probes layered with P. putida TVA8 were used to monitor liquid toluene over a two-week period. It was demonstrated that OFE probes layered with positively induced P. putida TVA8 bioreporters were reliable detectors of toluene. A toluene concentration of 26.5 mg/L was detected after <30 min after immersion of the probe in the toluene solution. Additional experiments also immobilized constitutively bioluminescent cells of E. coli 652T7, on OFEs with polyethyleneimine (PEI). These OFEs were repetitively induced with Lauria-Bertani (LB) nutrient medium. Bioluminescence appeared 15 minutes after immersion of the OFE in LB. A change in pH from 7 to 6 resulted in a decrease in bioluminescence that was not restored following additional nutrient inductions at pH 7. The E. coli 652T7 OFE probe was therefore sensitive to negative influences but could not be repetitively used. |
format | Online Article Text |
id | pubmed-7309017 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73090172020-06-25 Repetitive Detection of Aromatic Hydrocarbon Contaminants with Bioluminescent Bioreporters Attached on Tapered Optical Fiber Elements Zajíc, Jakub Ripp, Steven Trögl, Josef Kuncová, Gabriela Pospíšilová, Marie Sensors (Basel) Article In this study, we show the repetitive detection of toluene on a tapered optical fiber element (OFE) with an attached layer of Pseudomonas putida TVA8 bioluminescent bioreporters. The bioluminescent cell layer was attached on polished quartz modified with (3-aminopropyl)triethoxysilane (APTES). The repeatability of the preparation of the optical probe and its use was demonstrated with five differently shaped OFEs. The intensity of measured bioluminescence was minimally influenced by the OFE shape, possessing transmittances between 1.41% and 5.00%. OFE probes layered with P. putida TVA8 were used to monitor liquid toluene over a two-week period. It was demonstrated that OFE probes layered with positively induced P. putida TVA8 bioreporters were reliable detectors of toluene. A toluene concentration of 26.5 mg/L was detected after <30 min after immersion of the probe in the toluene solution. Additional experiments also immobilized constitutively bioluminescent cells of E. coli 652T7, on OFEs with polyethyleneimine (PEI). These OFEs were repetitively induced with Lauria-Bertani (LB) nutrient medium. Bioluminescence appeared 15 minutes after immersion of the OFE in LB. A change in pH from 7 to 6 resulted in a decrease in bioluminescence that was not restored following additional nutrient inductions at pH 7. The E. coli 652T7 OFE probe was therefore sensitive to negative influences but could not be repetitively used. MDPI 2020-06-06 /pmc/articles/PMC7309017/ /pubmed/32517218 http://dx.doi.org/10.3390/s20113237 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zajíc, Jakub Ripp, Steven Trögl, Josef Kuncová, Gabriela Pospíšilová, Marie Repetitive Detection of Aromatic Hydrocarbon Contaminants with Bioluminescent Bioreporters Attached on Tapered Optical Fiber Elements |
title | Repetitive Detection of Aromatic Hydrocarbon Contaminants with Bioluminescent Bioreporters Attached on Tapered Optical Fiber Elements |
title_full | Repetitive Detection of Aromatic Hydrocarbon Contaminants with Bioluminescent Bioreporters Attached on Tapered Optical Fiber Elements |
title_fullStr | Repetitive Detection of Aromatic Hydrocarbon Contaminants with Bioluminescent Bioreporters Attached on Tapered Optical Fiber Elements |
title_full_unstemmed | Repetitive Detection of Aromatic Hydrocarbon Contaminants with Bioluminescent Bioreporters Attached on Tapered Optical Fiber Elements |
title_short | Repetitive Detection of Aromatic Hydrocarbon Contaminants with Bioluminescent Bioreporters Attached on Tapered Optical Fiber Elements |
title_sort | repetitive detection of aromatic hydrocarbon contaminants with bioluminescent bioreporters attached on tapered optical fiber elements |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7309017/ https://www.ncbi.nlm.nih.gov/pubmed/32517218 http://dx.doi.org/10.3390/s20113237 |
work_keys_str_mv | AT zajicjakub repetitivedetectionofaromatichydrocarboncontaminantswithbioluminescentbioreportersattachedontaperedopticalfiberelements AT rippsteven repetitivedetectionofaromatichydrocarboncontaminantswithbioluminescentbioreportersattachedontaperedopticalfiberelements AT trogljosef repetitivedetectionofaromatichydrocarboncontaminantswithbioluminescentbioreportersattachedontaperedopticalfiberelements AT kuncovagabriela repetitivedetectionofaromatichydrocarboncontaminantswithbioluminescentbioreportersattachedontaperedopticalfiberelements AT pospisilovamarie repetitivedetectionofaromatichydrocarboncontaminantswithbioluminescentbioreportersattachedontaperedopticalfiberelements |