Cargando…

Repetitive Detection of Aromatic Hydrocarbon Contaminants with Bioluminescent Bioreporters Attached on Tapered Optical Fiber Elements

In this study, we show the repetitive detection of toluene on a tapered optical fiber element (OFE) with an attached layer of Pseudomonas putida TVA8 bioluminescent bioreporters. The bioluminescent cell layer was attached on polished quartz modified with (3-aminopropyl)triethoxysilane (APTES). The r...

Descripción completa

Detalles Bibliográficos
Autores principales: Zajíc, Jakub, Ripp, Steven, Trögl, Josef, Kuncová, Gabriela, Pospíšilová, Marie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7309017/
https://www.ncbi.nlm.nih.gov/pubmed/32517218
http://dx.doi.org/10.3390/s20113237
_version_ 1783549126617595904
author Zajíc, Jakub
Ripp, Steven
Trögl, Josef
Kuncová, Gabriela
Pospíšilová, Marie
author_facet Zajíc, Jakub
Ripp, Steven
Trögl, Josef
Kuncová, Gabriela
Pospíšilová, Marie
author_sort Zajíc, Jakub
collection PubMed
description In this study, we show the repetitive detection of toluene on a tapered optical fiber element (OFE) with an attached layer of Pseudomonas putida TVA8 bioluminescent bioreporters. The bioluminescent cell layer was attached on polished quartz modified with (3-aminopropyl)triethoxysilane (APTES). The repeatability of the preparation of the optical probe and its use was demonstrated with five differently shaped OFEs. The intensity of measured bioluminescence was minimally influenced by the OFE shape, possessing transmittances between 1.41% and 5.00%. OFE probes layered with P. putida TVA8 were used to monitor liquid toluene over a two-week period. It was demonstrated that OFE probes layered with positively induced P. putida TVA8 bioreporters were reliable detectors of toluene. A toluene concentration of 26.5 mg/L was detected after <30 min after immersion of the probe in the toluene solution. Additional experiments also immobilized constitutively bioluminescent cells of E. coli 652T7, on OFEs with polyethyleneimine (PEI). These OFEs were repetitively induced with Lauria-Bertani (LB) nutrient medium. Bioluminescence appeared 15 minutes after immersion of the OFE in LB. A change in pH from 7 to 6 resulted in a decrease in bioluminescence that was not restored following additional nutrient inductions at pH 7. The E. coli 652T7 OFE probe was therefore sensitive to negative influences but could not be repetitively used.
format Online
Article
Text
id pubmed-7309017
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-73090172020-06-25 Repetitive Detection of Aromatic Hydrocarbon Contaminants with Bioluminescent Bioreporters Attached on Tapered Optical Fiber Elements Zajíc, Jakub Ripp, Steven Trögl, Josef Kuncová, Gabriela Pospíšilová, Marie Sensors (Basel) Article In this study, we show the repetitive detection of toluene on a tapered optical fiber element (OFE) with an attached layer of Pseudomonas putida TVA8 bioluminescent bioreporters. The bioluminescent cell layer was attached on polished quartz modified with (3-aminopropyl)triethoxysilane (APTES). The repeatability of the preparation of the optical probe and its use was demonstrated with five differently shaped OFEs. The intensity of measured bioluminescence was minimally influenced by the OFE shape, possessing transmittances between 1.41% and 5.00%. OFE probes layered with P. putida TVA8 were used to monitor liquid toluene over a two-week period. It was demonstrated that OFE probes layered with positively induced P. putida TVA8 bioreporters were reliable detectors of toluene. A toluene concentration of 26.5 mg/L was detected after <30 min after immersion of the probe in the toluene solution. Additional experiments also immobilized constitutively bioluminescent cells of E. coli 652T7, on OFEs with polyethyleneimine (PEI). These OFEs were repetitively induced with Lauria-Bertani (LB) nutrient medium. Bioluminescence appeared 15 minutes after immersion of the OFE in LB. A change in pH from 7 to 6 resulted in a decrease in bioluminescence that was not restored following additional nutrient inductions at pH 7. The E. coli 652T7 OFE probe was therefore sensitive to negative influences but could not be repetitively used. MDPI 2020-06-06 /pmc/articles/PMC7309017/ /pubmed/32517218 http://dx.doi.org/10.3390/s20113237 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zajíc, Jakub
Ripp, Steven
Trögl, Josef
Kuncová, Gabriela
Pospíšilová, Marie
Repetitive Detection of Aromatic Hydrocarbon Contaminants with Bioluminescent Bioreporters Attached on Tapered Optical Fiber Elements
title Repetitive Detection of Aromatic Hydrocarbon Contaminants with Bioluminescent Bioreporters Attached on Tapered Optical Fiber Elements
title_full Repetitive Detection of Aromatic Hydrocarbon Contaminants with Bioluminescent Bioreporters Attached on Tapered Optical Fiber Elements
title_fullStr Repetitive Detection of Aromatic Hydrocarbon Contaminants with Bioluminescent Bioreporters Attached on Tapered Optical Fiber Elements
title_full_unstemmed Repetitive Detection of Aromatic Hydrocarbon Contaminants with Bioluminescent Bioreporters Attached on Tapered Optical Fiber Elements
title_short Repetitive Detection of Aromatic Hydrocarbon Contaminants with Bioluminescent Bioreporters Attached on Tapered Optical Fiber Elements
title_sort repetitive detection of aromatic hydrocarbon contaminants with bioluminescent bioreporters attached on tapered optical fiber elements
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7309017/
https://www.ncbi.nlm.nih.gov/pubmed/32517218
http://dx.doi.org/10.3390/s20113237
work_keys_str_mv AT zajicjakub repetitivedetectionofaromatichydrocarboncontaminantswithbioluminescentbioreportersattachedontaperedopticalfiberelements
AT rippsteven repetitivedetectionofaromatichydrocarboncontaminantswithbioluminescentbioreportersattachedontaperedopticalfiberelements
AT trogljosef repetitivedetectionofaromatichydrocarboncontaminantswithbioluminescentbioreportersattachedontaperedopticalfiberelements
AT kuncovagabriela repetitivedetectionofaromatichydrocarboncontaminantswithbioluminescentbioreportersattachedontaperedopticalfiberelements
AT pospisilovamarie repetitivedetectionofaromatichydrocarboncontaminantswithbioluminescentbioreportersattachedontaperedopticalfiberelements