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Electrical Modeling of the Growth and Differentiation of Skeletal Myoblasts Cell Cultures for Tissue Engineering
In tissue engineering, of utmost importance is the control of tissue formation, in order to form tissue constructs of clinical relevance. In this work, we present the use of an impedance spectroscopy technique for the real-time measurement of the dielectric properties of skeletal myoblast cell cultu...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7309147/ https://www.ncbi.nlm.nih.gov/pubmed/32498394 http://dx.doi.org/10.3390/s20113152 |
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author | Olmo, Alberto Yuste, Yaiza Serrano, Juan Alfonso Maldonado-Jacobi, Andres Pérez, Pablo Huertas, Gloria Pereira, Sheila Yufera, Alberto de la Portilla, Fernando |
author_facet | Olmo, Alberto Yuste, Yaiza Serrano, Juan Alfonso Maldonado-Jacobi, Andres Pérez, Pablo Huertas, Gloria Pereira, Sheila Yufera, Alberto de la Portilla, Fernando |
author_sort | Olmo, Alberto |
collection | PubMed |
description | In tissue engineering, of utmost importance is the control of tissue formation, in order to form tissue constructs of clinical relevance. In this work, we present the use of an impedance spectroscopy technique for the real-time measurement of the dielectric properties of skeletal myoblast cell cultures. The processes involved in the growth and differentiation of these cell cultures in skeletal muscle are studied. A circuit based on the oscillation-based test technique was used, avoiding the use of high-performance circuitry or external input signals. The effect of electrical pulse stimulation applied to cell cultures was also studied. The technique proved useful for monitoring in real-time the processes of cell growth and estimating the fill factor of muscular stem cells. Impedance spectroscopy was also useful to study the real-time monitoring of cell differentiation, obtaining different oscillation amplitude levels for differentiated and undifferentiated cell cultures. Finally, an electrical model was implemented to better understand the physical properties of the cell culture and control the tissue formation process. |
format | Online Article Text |
id | pubmed-7309147 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73091472020-06-25 Electrical Modeling of the Growth and Differentiation of Skeletal Myoblasts Cell Cultures for Tissue Engineering Olmo, Alberto Yuste, Yaiza Serrano, Juan Alfonso Maldonado-Jacobi, Andres Pérez, Pablo Huertas, Gloria Pereira, Sheila Yufera, Alberto de la Portilla, Fernando Sensors (Basel) Article In tissue engineering, of utmost importance is the control of tissue formation, in order to form tissue constructs of clinical relevance. In this work, we present the use of an impedance spectroscopy technique for the real-time measurement of the dielectric properties of skeletal myoblast cell cultures. The processes involved in the growth and differentiation of these cell cultures in skeletal muscle are studied. A circuit based on the oscillation-based test technique was used, avoiding the use of high-performance circuitry or external input signals. The effect of electrical pulse stimulation applied to cell cultures was also studied. The technique proved useful for monitoring in real-time the processes of cell growth and estimating the fill factor of muscular stem cells. Impedance spectroscopy was also useful to study the real-time monitoring of cell differentiation, obtaining different oscillation amplitude levels for differentiated and undifferentiated cell cultures. Finally, an electrical model was implemented to better understand the physical properties of the cell culture and control the tissue formation process. MDPI 2020-06-02 /pmc/articles/PMC7309147/ /pubmed/32498394 http://dx.doi.org/10.3390/s20113152 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Olmo, Alberto Yuste, Yaiza Serrano, Juan Alfonso Maldonado-Jacobi, Andres Pérez, Pablo Huertas, Gloria Pereira, Sheila Yufera, Alberto de la Portilla, Fernando Electrical Modeling of the Growth and Differentiation of Skeletal Myoblasts Cell Cultures for Tissue Engineering |
title | Electrical Modeling of the Growth and Differentiation of Skeletal Myoblasts Cell Cultures for Tissue Engineering |
title_full | Electrical Modeling of the Growth and Differentiation of Skeletal Myoblasts Cell Cultures for Tissue Engineering |
title_fullStr | Electrical Modeling of the Growth and Differentiation of Skeletal Myoblasts Cell Cultures for Tissue Engineering |
title_full_unstemmed | Electrical Modeling of the Growth and Differentiation of Skeletal Myoblasts Cell Cultures for Tissue Engineering |
title_short | Electrical Modeling of the Growth and Differentiation of Skeletal Myoblasts Cell Cultures for Tissue Engineering |
title_sort | electrical modeling of the growth and differentiation of skeletal myoblasts cell cultures for tissue engineering |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7309147/ https://www.ncbi.nlm.nih.gov/pubmed/32498394 http://dx.doi.org/10.3390/s20113152 |
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