Cargando…

Electrical Modeling of the Growth and Differentiation of Skeletal Myoblasts Cell Cultures for Tissue Engineering

In tissue engineering, of utmost importance is the control of tissue formation, in order to form tissue constructs of clinical relevance. In this work, we present the use of an impedance spectroscopy technique for the real-time measurement of the dielectric properties of skeletal myoblast cell cultu...

Descripción completa

Detalles Bibliográficos
Autores principales: Olmo, Alberto, Yuste, Yaiza, Serrano, Juan Alfonso, Maldonado-Jacobi, Andres, Pérez, Pablo, Huertas, Gloria, Pereira, Sheila, Yufera, Alberto, de la Portilla, Fernando
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7309147/
https://www.ncbi.nlm.nih.gov/pubmed/32498394
http://dx.doi.org/10.3390/s20113152
_version_ 1783549157915492352
author Olmo, Alberto
Yuste, Yaiza
Serrano, Juan Alfonso
Maldonado-Jacobi, Andres
Pérez, Pablo
Huertas, Gloria
Pereira, Sheila
Yufera, Alberto
de la Portilla, Fernando
author_facet Olmo, Alberto
Yuste, Yaiza
Serrano, Juan Alfonso
Maldonado-Jacobi, Andres
Pérez, Pablo
Huertas, Gloria
Pereira, Sheila
Yufera, Alberto
de la Portilla, Fernando
author_sort Olmo, Alberto
collection PubMed
description In tissue engineering, of utmost importance is the control of tissue formation, in order to form tissue constructs of clinical relevance. In this work, we present the use of an impedance spectroscopy technique for the real-time measurement of the dielectric properties of skeletal myoblast cell cultures. The processes involved in the growth and differentiation of these cell cultures in skeletal muscle are studied. A circuit based on the oscillation-based test technique was used, avoiding the use of high-performance circuitry or external input signals. The effect of electrical pulse stimulation applied to cell cultures was also studied. The technique proved useful for monitoring in real-time the processes of cell growth and estimating the fill factor of muscular stem cells. Impedance spectroscopy was also useful to study the real-time monitoring of cell differentiation, obtaining different oscillation amplitude levels for differentiated and undifferentiated cell cultures. Finally, an electrical model was implemented to better understand the physical properties of the cell culture and control the tissue formation process.
format Online
Article
Text
id pubmed-7309147
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-73091472020-06-25 Electrical Modeling of the Growth and Differentiation of Skeletal Myoblasts Cell Cultures for Tissue Engineering Olmo, Alberto Yuste, Yaiza Serrano, Juan Alfonso Maldonado-Jacobi, Andres Pérez, Pablo Huertas, Gloria Pereira, Sheila Yufera, Alberto de la Portilla, Fernando Sensors (Basel) Article In tissue engineering, of utmost importance is the control of tissue formation, in order to form tissue constructs of clinical relevance. In this work, we present the use of an impedance spectroscopy technique for the real-time measurement of the dielectric properties of skeletal myoblast cell cultures. The processes involved in the growth and differentiation of these cell cultures in skeletal muscle are studied. A circuit based on the oscillation-based test technique was used, avoiding the use of high-performance circuitry or external input signals. The effect of electrical pulse stimulation applied to cell cultures was also studied. The technique proved useful for monitoring in real-time the processes of cell growth and estimating the fill factor of muscular stem cells. Impedance spectroscopy was also useful to study the real-time monitoring of cell differentiation, obtaining different oscillation amplitude levels for differentiated and undifferentiated cell cultures. Finally, an electrical model was implemented to better understand the physical properties of the cell culture and control the tissue formation process. MDPI 2020-06-02 /pmc/articles/PMC7309147/ /pubmed/32498394 http://dx.doi.org/10.3390/s20113152 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Olmo, Alberto
Yuste, Yaiza
Serrano, Juan Alfonso
Maldonado-Jacobi, Andres
Pérez, Pablo
Huertas, Gloria
Pereira, Sheila
Yufera, Alberto
de la Portilla, Fernando
Electrical Modeling of the Growth and Differentiation of Skeletal Myoblasts Cell Cultures for Tissue Engineering
title Electrical Modeling of the Growth and Differentiation of Skeletal Myoblasts Cell Cultures for Tissue Engineering
title_full Electrical Modeling of the Growth and Differentiation of Skeletal Myoblasts Cell Cultures for Tissue Engineering
title_fullStr Electrical Modeling of the Growth and Differentiation of Skeletal Myoblasts Cell Cultures for Tissue Engineering
title_full_unstemmed Electrical Modeling of the Growth and Differentiation of Skeletal Myoblasts Cell Cultures for Tissue Engineering
title_short Electrical Modeling of the Growth and Differentiation of Skeletal Myoblasts Cell Cultures for Tissue Engineering
title_sort electrical modeling of the growth and differentiation of skeletal myoblasts cell cultures for tissue engineering
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7309147/
https://www.ncbi.nlm.nih.gov/pubmed/32498394
http://dx.doi.org/10.3390/s20113152
work_keys_str_mv AT olmoalberto electricalmodelingofthegrowthanddifferentiationofskeletalmyoblastscellculturesfortissueengineering
AT yusteyaiza electricalmodelingofthegrowthanddifferentiationofskeletalmyoblastscellculturesfortissueengineering
AT serranojuanalfonso electricalmodelingofthegrowthanddifferentiationofskeletalmyoblastscellculturesfortissueengineering
AT maldonadojacobiandres electricalmodelingofthegrowthanddifferentiationofskeletalmyoblastscellculturesfortissueengineering
AT perezpablo electricalmodelingofthegrowthanddifferentiationofskeletalmyoblastscellculturesfortissueengineering
AT huertasgloria electricalmodelingofthegrowthanddifferentiationofskeletalmyoblastscellculturesfortissueengineering
AT pereirasheila electricalmodelingofthegrowthanddifferentiationofskeletalmyoblastscellculturesfortissueengineering
AT yuferaalberto electricalmodelingofthegrowthanddifferentiationofskeletalmyoblastscellculturesfortissueengineering
AT delaportillafernando electricalmodelingofthegrowthanddifferentiationofskeletalmyoblastscellculturesfortissueengineering