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Conductive collagen/polypyrrole-b-polycaprolactone hydrogel for bioprinting of neural tissue constructs

Bioprinting is increasingly being used for fabrication of engineered tissues for regenerative medicine, drug testing, and other biomedical applications. The success of this technology lies with the development of suitable bioinks and hydrogels that are specific to the intended tissue application. Fo...

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Autores principales: Vijayavenkataraman, Sanjairaj, Vialli, Novelia, Fuh, Jerry Y. H., Lu, Wen Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Whioce Publishing Pte. Ltd. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7310269/
https://www.ncbi.nlm.nih.gov/pubmed/32596545
http://dx.doi.org/10.18063/ijb.v5i2.1.229
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author Vijayavenkataraman, Sanjairaj
Vialli, Novelia
Fuh, Jerry Y. H.
Lu, Wen Feng
author_facet Vijayavenkataraman, Sanjairaj
Vialli, Novelia
Fuh, Jerry Y. H.
Lu, Wen Feng
author_sort Vijayavenkataraman, Sanjairaj
collection PubMed
description Bioprinting is increasingly being used for fabrication of engineered tissues for regenerative medicine, drug testing, and other biomedical applications. The success of this technology lies with the development of suitable bioinks and hydrogels that are specific to the intended tissue application. For applications such as neural tissue engineering, conductivity plays an important role in determining the neural differentiation and neural tissue regeneration. Although several conductive hydrogels based on metal nanoparticles (NPs) such as gold and silver, carbon-based materials such as graphene and carbon nanotubes and conducting polymers such as polypyrrole (PPy) and polyaniline were used, they possess several disadvantages. The long-term cytotoxicity of metal nanoparticles (NPs) and carbon-based materials restricts their use in regenerative medicine. The conductive polymers, on the other hand, are non-biodegradable and possess weak mechanical properties limiting their printability into three-dimensional constructs. The aim of this study is to develop a biodegradable, conductive, and printable hydrogel based on collagen and a block copolymer of PPy and polycaprolactone (PCL) (PPy-block-poly(caprolactone) [PPy-b-PCL]) for bioprinting of neural tissue constructs. The printability, including the influence of the printing speed and material flow rate on the printed fiber width; rheological properties; and cytotoxicity of these hydrogels were studied. The results prove that the collagen/PPy-b-PCL hydrogels possessed better printability and biocompatibility. Thus, the collagen/PPy-b-PCL hydrogels reported this study has the potential to be used in the bioprinting of neural tissue constructs for the repair of damaged neural tissues and drug testing or precision medicine applications.
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spelling pubmed-73102692020-06-25 Conductive collagen/polypyrrole-b-polycaprolactone hydrogel for bioprinting of neural tissue constructs Vijayavenkataraman, Sanjairaj Vialli, Novelia Fuh, Jerry Y. H. Lu, Wen Feng Int J Bioprint Research Article Bioprinting is increasingly being used for fabrication of engineered tissues for regenerative medicine, drug testing, and other biomedical applications. The success of this technology lies with the development of suitable bioinks and hydrogels that are specific to the intended tissue application. For applications such as neural tissue engineering, conductivity plays an important role in determining the neural differentiation and neural tissue regeneration. Although several conductive hydrogels based on metal nanoparticles (NPs) such as gold and silver, carbon-based materials such as graphene and carbon nanotubes and conducting polymers such as polypyrrole (PPy) and polyaniline were used, they possess several disadvantages. The long-term cytotoxicity of metal nanoparticles (NPs) and carbon-based materials restricts their use in regenerative medicine. The conductive polymers, on the other hand, are non-biodegradable and possess weak mechanical properties limiting their printability into three-dimensional constructs. The aim of this study is to develop a biodegradable, conductive, and printable hydrogel based on collagen and a block copolymer of PPy and polycaprolactone (PCL) (PPy-block-poly(caprolactone) [PPy-b-PCL]) for bioprinting of neural tissue constructs. The printability, including the influence of the printing speed and material flow rate on the printed fiber width; rheological properties; and cytotoxicity of these hydrogels were studied. The results prove that the collagen/PPy-b-PCL hydrogels possessed better printability and biocompatibility. Thus, the collagen/PPy-b-PCL hydrogels reported this study has the potential to be used in the bioprinting of neural tissue constructs for the repair of damaged neural tissues and drug testing or precision medicine applications. Whioce Publishing Pte. Ltd. 2019-07-11 /pmc/articles/PMC7310269/ /pubmed/32596545 http://dx.doi.org/10.18063/ijb.v5i2.1.229 Text en Copyright: © 2019 Vijayavenkataraman S, et al. http://creativecommons.org/licenses/cc-by-nc/4.0/ This is an open-access article distributed under the terms of the Attribution-NonCommercial 4.0 International 4.0 (CC BY-NC 4.0), which permits all non-commercial use, distribution, and reproduction in any medium provided the original work is properly cited.
spellingShingle Research Article
Vijayavenkataraman, Sanjairaj
Vialli, Novelia
Fuh, Jerry Y. H.
Lu, Wen Feng
Conductive collagen/polypyrrole-b-polycaprolactone hydrogel for bioprinting of neural tissue constructs
title Conductive collagen/polypyrrole-b-polycaprolactone hydrogel for bioprinting of neural tissue constructs
title_full Conductive collagen/polypyrrole-b-polycaprolactone hydrogel for bioprinting of neural tissue constructs
title_fullStr Conductive collagen/polypyrrole-b-polycaprolactone hydrogel for bioprinting of neural tissue constructs
title_full_unstemmed Conductive collagen/polypyrrole-b-polycaprolactone hydrogel for bioprinting of neural tissue constructs
title_short Conductive collagen/polypyrrole-b-polycaprolactone hydrogel for bioprinting of neural tissue constructs
title_sort conductive collagen/polypyrrole-b-polycaprolactone hydrogel for bioprinting of neural tissue constructs
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7310269/
https://www.ncbi.nlm.nih.gov/pubmed/32596545
http://dx.doi.org/10.18063/ijb.v5i2.1.229
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