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Identification of plasmid IncQ1 and NTE(KPC)-IId harboring bla (KPC-2) in isolates from Klebsiella pneumoniae infections in patients from Recife-PE, Brazil

INTRODUCTION: This study investigated the genetic environment of bla (KPC-2) in Klebsiella pnemoniae multi-drug resistant clinical isolates. METHODS: Four carbapenemase gene isolates resistant to carbapenems, collected from infected patients from two hospitals in Brazil, were investigated using poly...

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Detalles Bibliográficos
Autores principales: de Lima, Giselle Jucá, Scavuzzi, Alexsandra Maria Lima, Beltrão, Elizabeth Maria Bispo, Firmo, Elza Ferreira, de Oliveira, Érica Maria, de Oliveira, Sibele Ribeiro, Rezende, Antônio Mauro, Lopes, Ana Catarina de Souza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Medicina Tropical - SBMT 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7310361/
https://www.ncbi.nlm.nih.gov/pubmed/32578705
http://dx.doi.org/10.1590/0037-8682-0526-2019
Descripción
Sumario:INTRODUCTION: This study investigated the genetic environment of bla (KPC-2) in Klebsiella pnemoniae multi-drug resistant clinical isolates. METHODS: Four carbapenemase gene isolates resistant to carbapenems, collected from infected patients from two hospitals in Brazil, were investigated using polymerase chain reaction and plasmid DNA sequencing. RESULTS: The bla (KPC-2) gene was located between ISKpn6 and a resolvase tnpR in the non-Tn4401 element (NTE(KPC-)IId). It was detected on a plasmid belonging to the IncQ1 group. CONCLUSIONS: To our knowledge, this is the first report of the presence of the bla (KPC-2) gene in the NTE(KPC)-IId element carried by plasmid IncQ1 from infections in Brazil.