Genomic surveillance of the Chikungunya Virus (CHIKV) in Northeast Brazil after the first outbreak in 2014

INTRODUCTION: We performed an epidemiological surveillance of the Chikungunya (CHIKV) lineages in Bahia after the 2014 East/Central/South African (ECSA) genotype outbreak. METHODS: Reverse-transcription polymerase chain reaction (RT-PCR), viral isolation, and phylogenetic analyses were conducted on...

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Autores principales: Rodrigues, Ayslany Melo, Souza, Rafael Ribeiro Mota, Fonseca, Larissa Moraes dos Santos, Rolo, Carolina de Araújo, Carvalho, Rejane Hughes, Sardi, Silvia Ines, Campos, Gubio Soares
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Medicina Tropical - SBMT 2020
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7310362/
https://www.ncbi.nlm.nih.gov/pubmed/32578706
http://dx.doi.org/10.1590/0037-8682-0583-2019
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author Rodrigues, Ayslany Melo
Souza, Rafael Ribeiro Mota
Fonseca, Larissa Moraes dos Santos
Rolo, Carolina de Araújo
Carvalho, Rejane Hughes
Sardi, Silvia Ines
Campos, Gubio Soares
author_facet Rodrigues, Ayslany Melo
Souza, Rafael Ribeiro Mota
Fonseca, Larissa Moraes dos Santos
Rolo, Carolina de Araújo
Carvalho, Rejane Hughes
Sardi, Silvia Ines
Campos, Gubio Soares
author_sort Rodrigues, Ayslany Melo
collection PubMed
description INTRODUCTION: We performed an epidemiological surveillance of the Chikungunya (CHIKV) lineages in Bahia after the 2014 East/Central/South African (ECSA) genotype outbreak. METHODS: Reverse-transcription polymerase chain reaction (RT-PCR), viral isolation, and phylogenetic analyses were conducted on serum samples from 605 patients with CHIKV-like symptoms during 2014-2018. RESULTS: Of the 605 samples, 167 were CHIKV-positive. Viral isolation was achieved for 20 samples; their phylogenetic analysis (E2 protein) revealed the presence of ECSA lineage and reinforced the phylogenetic relationship between ECSA and Indian Ocean lineages. CONCLUSIONS: The genomic surveillance of CHIKV showed that only ECSA lineage circulated in Bahia since the 2014 outbreak.
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spelling pubmed-73103622020-06-24 Genomic surveillance of the Chikungunya Virus (CHIKV) in Northeast Brazil after the first outbreak in 2014 Rodrigues, Ayslany Melo Souza, Rafael Ribeiro Mota Fonseca, Larissa Moraes dos Santos Rolo, Carolina de Araújo Carvalho, Rejane Hughes Sardi, Silvia Ines Campos, Gubio Soares Rev Soc Bras Med Trop Short Communication INTRODUCTION: We performed an epidemiological surveillance of the Chikungunya (CHIKV) lineages in Bahia after the 2014 East/Central/South African (ECSA) genotype outbreak. METHODS: Reverse-transcription polymerase chain reaction (RT-PCR), viral isolation, and phylogenetic analyses were conducted on serum samples from 605 patients with CHIKV-like symptoms during 2014-2018. RESULTS: Of the 605 samples, 167 were CHIKV-positive. Viral isolation was achieved for 20 samples; their phylogenetic analysis (E2 protein) revealed the presence of ECSA lineage and reinforced the phylogenetic relationship between ECSA and Indian Ocean lineages. CONCLUSIONS: The genomic surveillance of CHIKV showed that only ECSA lineage circulated in Bahia since the 2014 outbreak. Sociedade Brasileira de Medicina Tropical - SBMT 2020-06-22 /pmc/articles/PMC7310362/ /pubmed/32578706 http://dx.doi.org/10.1590/0037-8682-0583-2019 Text en https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License
spellingShingle Short Communication
Rodrigues, Ayslany Melo
Souza, Rafael Ribeiro Mota
Fonseca, Larissa Moraes dos Santos
Rolo, Carolina de Araújo
Carvalho, Rejane Hughes
Sardi, Silvia Ines
Campos, Gubio Soares
Genomic surveillance of the Chikungunya Virus (CHIKV) in Northeast Brazil after the first outbreak in 2014
title Genomic surveillance of the Chikungunya Virus (CHIKV) in Northeast Brazil after the first outbreak in 2014
title_full Genomic surveillance of the Chikungunya Virus (CHIKV) in Northeast Brazil after the first outbreak in 2014
title_fullStr Genomic surveillance of the Chikungunya Virus (CHIKV) in Northeast Brazil after the first outbreak in 2014
title_full_unstemmed Genomic surveillance of the Chikungunya Virus (CHIKV) in Northeast Brazil after the first outbreak in 2014
title_short Genomic surveillance of the Chikungunya Virus (CHIKV) in Northeast Brazil after the first outbreak in 2014
title_sort genomic surveillance of the chikungunya virus (chikv) in northeast brazil after the first outbreak in 2014
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7310362/
https://www.ncbi.nlm.nih.gov/pubmed/32578706
http://dx.doi.org/10.1590/0037-8682-0583-2019
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