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Detection of Antibody and Antigen for Lassa Virus Nucleoprotein in Monkeys from Southern Nigeria

Lassa fever is a deadly viral haemorrhagic fever caused by Lassa Virus (LASV). Rodents, especially, Mystomys natalensis, are the known reservoirs of LASV and humans are the defined hosts. Monkeys share many illnesses with humans and experimental LASV infections in monkeys are fatal but natural LASV...

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Autores principales: Ogunro, Bamidele Nyemike, Olugasa, Babasola Oluseyi, Kayode, Adeyemi, Ishola, Olayinka Olabisi, Kolawole, Oluseyi Noah, Odigie, Eugene Amiewanlen, Happi, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Atlantis Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7310747/
https://www.ncbi.nlm.nih.gov/pubmed/31241870
http://dx.doi.org/10.2991/jegh.k.190421.001
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author Ogunro, Bamidele Nyemike
Olugasa, Babasola Oluseyi
Kayode, Adeyemi
Ishola, Olayinka Olabisi
Kolawole, Oluseyi Noah
Odigie, Eugene Amiewanlen
Happi, Christian
author_facet Ogunro, Bamidele Nyemike
Olugasa, Babasola Oluseyi
Kayode, Adeyemi
Ishola, Olayinka Olabisi
Kolawole, Oluseyi Noah
Odigie, Eugene Amiewanlen
Happi, Christian
author_sort Ogunro, Bamidele Nyemike
collection PubMed
description Lassa fever is a deadly viral haemorrhagic fever caused by Lassa Virus (LASV). Rodents, especially, Mystomys natalensis, are the known reservoirs of LASV and humans are the defined hosts. Monkeys share many illnesses with humans and experimental LASV infections in monkeys are fatal but natural LASV infection of monkeys has not been reported. Serum samples obtained between August 2015 and December 2017 from 62 monkeys belonging to six species in Southern Nigeria were tested for LASV as part of an ongoing surveillance of monkeys in the region for zoonotic pathogens. Commercially available Recombinant LASV (ReLASV) Pan-Lassa enzyme-linked immunosorbent assay (ELISA) test kits (Zalgen Labs, Germantown, MD, USA) were used to detect antibodies (IgG and IgM) and antigen specific for LASV nucleoprotein in the sera. Lassa-fever-specific IgG and IgM, and antigen specific for LASV nucleoprotein were detected in 5/62, 0/62, and 1/62 samples, respectively. The presence of LASV-specific antibodies in the sera suggests natural exposure to the virus, while the presence of LASV antigen may mean that monkeys are carriers of the virus. There is a need to broaden Lassa fever surveillance to include nonhuman primates (NHPs) for their probable role in the epidemiology of the disease. HIGHLIGHTS: • Rodents are the natural reservoirs of Lassa fever virus (LASV) and humans are the defined hosts. • Experimental LASV infections in non-human primates (NHP) are fatal but natural infection of NHP with the virus have not been reported. • We detected antigen and antibody specific for LASV in free-living Monkeys from southern Nigeria which implies that monkeys in the region are naturally exposed to LASV and are probable carriers of the virus.
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spelling pubmed-73107472020-07-28 Detection of Antibody and Antigen for Lassa Virus Nucleoprotein in Monkeys from Southern Nigeria Ogunro, Bamidele Nyemike Olugasa, Babasola Oluseyi Kayode, Adeyemi Ishola, Olayinka Olabisi Kolawole, Oluseyi Noah Odigie, Eugene Amiewanlen Happi, Christian J Epidemiol Glob Health Research Article Lassa fever is a deadly viral haemorrhagic fever caused by Lassa Virus (LASV). Rodents, especially, Mystomys natalensis, are the known reservoirs of LASV and humans are the defined hosts. Monkeys share many illnesses with humans and experimental LASV infections in monkeys are fatal but natural LASV infection of monkeys has not been reported. Serum samples obtained between August 2015 and December 2017 from 62 monkeys belonging to six species in Southern Nigeria were tested for LASV as part of an ongoing surveillance of monkeys in the region for zoonotic pathogens. Commercially available Recombinant LASV (ReLASV) Pan-Lassa enzyme-linked immunosorbent assay (ELISA) test kits (Zalgen Labs, Germantown, MD, USA) were used to detect antibodies (IgG and IgM) and antigen specific for LASV nucleoprotein in the sera. Lassa-fever-specific IgG and IgM, and antigen specific for LASV nucleoprotein were detected in 5/62, 0/62, and 1/62 samples, respectively. The presence of LASV-specific antibodies in the sera suggests natural exposure to the virus, while the presence of LASV antigen may mean that monkeys are carriers of the virus. There is a need to broaden Lassa fever surveillance to include nonhuman primates (NHPs) for their probable role in the epidemiology of the disease. HIGHLIGHTS: • Rodents are the natural reservoirs of Lassa fever virus (LASV) and humans are the defined hosts. • Experimental LASV infections in non-human primates (NHP) are fatal but natural infection of NHP with the virus have not been reported. • We detected antigen and antibody specific for LASV in free-living Monkeys from southern Nigeria which implies that monkeys in the region are naturally exposed to LASV and are probable carriers of the virus. Atlantis Press 2019-06 2019-06 /pmc/articles/PMC7310747/ /pubmed/31241870 http://dx.doi.org/10.2991/jegh.k.190421.001 Text en © 2019 Atlantis Press International B.V. This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).
spellingShingle Research Article
Ogunro, Bamidele Nyemike
Olugasa, Babasola Oluseyi
Kayode, Adeyemi
Ishola, Olayinka Olabisi
Kolawole, Oluseyi Noah
Odigie, Eugene Amiewanlen
Happi, Christian
Detection of Antibody and Antigen for Lassa Virus Nucleoprotein in Monkeys from Southern Nigeria
title Detection of Antibody and Antigen for Lassa Virus Nucleoprotein in Monkeys from Southern Nigeria
title_full Detection of Antibody and Antigen for Lassa Virus Nucleoprotein in Monkeys from Southern Nigeria
title_fullStr Detection of Antibody and Antigen for Lassa Virus Nucleoprotein in Monkeys from Southern Nigeria
title_full_unstemmed Detection of Antibody and Antigen for Lassa Virus Nucleoprotein in Monkeys from Southern Nigeria
title_short Detection of Antibody and Antigen for Lassa Virus Nucleoprotein in Monkeys from Southern Nigeria
title_sort detection of antibody and antigen for lassa virus nucleoprotein in monkeys from southern nigeria
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7310747/
https://www.ncbi.nlm.nih.gov/pubmed/31241870
http://dx.doi.org/10.2991/jegh.k.190421.001
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