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Cytotoxic and Antimicrobial Efficacy of Silver Nanoparticles Synthesized Using a Traditional Phytoproduct, Asafoetida Gum

PURPOSE: The present study synthesized silver nanoparticles (AgNPs) using the aqueous extract of a traditional medicinal product consisting of an oleoresin (a combination of macromolecules of carbohydrates and proteins) exuded from the rhizome of the plant Ferula foetida (asafoetida gum) and evaluat...

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Autores principales: Devanesan, Sandhanasamy, Ponmurugan, Karuppiah, AlSalhi, Mohamad S, Al-Dhabi, Naif Abdullah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7310983/
https://www.ncbi.nlm.nih.gov/pubmed/32606682
http://dx.doi.org/10.2147/IJN.S258319
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author Devanesan, Sandhanasamy
Ponmurugan, Karuppiah
AlSalhi, Mohamad S
Al-Dhabi, Naif Abdullah
author_facet Devanesan, Sandhanasamy
Ponmurugan, Karuppiah
AlSalhi, Mohamad S
Al-Dhabi, Naif Abdullah
author_sort Devanesan, Sandhanasamy
collection PubMed
description PURPOSE: The present study synthesized silver nanoparticles (AgNPs) using the aqueous extract of a traditional medicinal product consisting of an oleoresin (a combination of macromolecules of carbohydrates and proteins) exuded from the rhizome of the plant Ferula foetida (asafoetida gum) and evaluated its biological properties. MATERIALS AND METHODS: The silver nanoparticles synthesized using asafoetida gum (As-AgNPs) were characterized using UV/Vis spectroscopy, fourier infrared (FTIR) spectroscopy, scanning electron microscopy (SEM) with energy dispersive X-ray analysis (EDX), X-ray diffraction (XRD) and transmission electron microscopy (TEM) and EADX. The cytotoxicity and antimicrobial activity As-AgNPs were evaluated against MCF-7 cell lines and selected microbial pathogens, respectively. RESULTS: The synthesized silver nanoparticles were crystalline in nature with a spherical shape. The average particle size was 5.6–8.6 nm. The cytotoxicity of the synthesized As-AgNPs was evaluated against MCF-7 cell lines, and the As-AgNPs were found to be effective in inhibiting the multiplication of cancer cells. The As-AgNPs exhibited significant antimicrobial activity towards E. coli, K. pneumoniae and C. albicans. The MIC of the synthesized As-AgNPs was 7.80 μg/mL for E. coli ATCC 25922, Salmonella sp. WS50- and S. typhi; 15.60 μg/mL for S. typhimurium and S. aureus WS10, and 31.20 μg/mL for K. pneumoniae and S. aureus ATCC 43300-MRSA. In addition, MIC values of 15.60 μg/mL for C. albicans ATCC8436 and 31.20 μg/mL for C. krusei ATCC6258 were obtained. CONCLUSION: As asafoetida is a good traditional medicine, its involvement in the synthesis of AgNPs led the silver nanoparticles to exhibit good cytotoxic and antimicrobial effects.
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spelling pubmed-73109832020-06-29 Cytotoxic and Antimicrobial Efficacy of Silver Nanoparticles Synthesized Using a Traditional Phytoproduct, Asafoetida Gum Devanesan, Sandhanasamy Ponmurugan, Karuppiah AlSalhi, Mohamad S Al-Dhabi, Naif Abdullah Int J Nanomedicine Original Research PURPOSE: The present study synthesized silver nanoparticles (AgNPs) using the aqueous extract of a traditional medicinal product consisting of an oleoresin (a combination of macromolecules of carbohydrates and proteins) exuded from the rhizome of the plant Ferula foetida (asafoetida gum) and evaluated its biological properties. MATERIALS AND METHODS: The silver nanoparticles synthesized using asafoetida gum (As-AgNPs) were characterized using UV/Vis spectroscopy, fourier infrared (FTIR) spectroscopy, scanning electron microscopy (SEM) with energy dispersive X-ray analysis (EDX), X-ray diffraction (XRD) and transmission electron microscopy (TEM) and EADX. The cytotoxicity and antimicrobial activity As-AgNPs were evaluated against MCF-7 cell lines and selected microbial pathogens, respectively. RESULTS: The synthesized silver nanoparticles were crystalline in nature with a spherical shape. The average particle size was 5.6–8.6 nm. The cytotoxicity of the synthesized As-AgNPs was evaluated against MCF-7 cell lines, and the As-AgNPs were found to be effective in inhibiting the multiplication of cancer cells. The As-AgNPs exhibited significant antimicrobial activity towards E. coli, K. pneumoniae and C. albicans. The MIC of the synthesized As-AgNPs was 7.80 μg/mL for E. coli ATCC 25922, Salmonella sp. WS50- and S. typhi; 15.60 μg/mL for S. typhimurium and S. aureus WS10, and 31.20 μg/mL for K. pneumoniae and S. aureus ATCC 43300-MRSA. In addition, MIC values of 15.60 μg/mL for C. albicans ATCC8436 and 31.20 μg/mL for C. krusei ATCC6258 were obtained. CONCLUSION: As asafoetida is a good traditional medicine, its involvement in the synthesis of AgNPs led the silver nanoparticles to exhibit good cytotoxic and antimicrobial effects. Dove 2020-06-19 /pmc/articles/PMC7310983/ /pubmed/32606682 http://dx.doi.org/10.2147/IJN.S258319 Text en © 2020 Devanesan et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Devanesan, Sandhanasamy
Ponmurugan, Karuppiah
AlSalhi, Mohamad S
Al-Dhabi, Naif Abdullah
Cytotoxic and Antimicrobial Efficacy of Silver Nanoparticles Synthesized Using a Traditional Phytoproduct, Asafoetida Gum
title Cytotoxic and Antimicrobial Efficacy of Silver Nanoparticles Synthesized Using a Traditional Phytoproduct, Asafoetida Gum
title_full Cytotoxic and Antimicrobial Efficacy of Silver Nanoparticles Synthesized Using a Traditional Phytoproduct, Asafoetida Gum
title_fullStr Cytotoxic and Antimicrobial Efficacy of Silver Nanoparticles Synthesized Using a Traditional Phytoproduct, Asafoetida Gum
title_full_unstemmed Cytotoxic and Antimicrobial Efficacy of Silver Nanoparticles Synthesized Using a Traditional Phytoproduct, Asafoetida Gum
title_short Cytotoxic and Antimicrobial Efficacy of Silver Nanoparticles Synthesized Using a Traditional Phytoproduct, Asafoetida Gum
title_sort cytotoxic and antimicrobial efficacy of silver nanoparticles synthesized using a traditional phytoproduct, asafoetida gum
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7310983/
https://www.ncbi.nlm.nih.gov/pubmed/32606682
http://dx.doi.org/10.2147/IJN.S258319
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