Cargando…
Low-Dose Exposure of Silica Nanoparticles Induces Neurotoxicity via Neuroactive Ligand–Receptor Interaction Signaling Pathway in Zebrafish Embryos
OBJECTIVE: Silica nanoparticles (SiO(2) NPs) have been extensively employed in biomedical field. SiO(2) NPs are primarily designed to enter the circulatory system; however, little information is available on potential adverse effects of SiO(2) NPs on the nervous system. METHODS: The neurotoxicity of...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7310985/ https://www.ncbi.nlm.nih.gov/pubmed/32606685 http://dx.doi.org/10.2147/IJN.S254480 |
_version_ | 1783549471928352768 |
---|---|
author | Wei, Jialiu Liu, Jianhui Liang, Shuang Sun, Mengqi Duan, Junchao |
author_facet | Wei, Jialiu Liu, Jianhui Liang, Shuang Sun, Mengqi Duan, Junchao |
author_sort | Wei, Jialiu |
collection | PubMed |
description | OBJECTIVE: Silica nanoparticles (SiO(2) NPs) have been extensively employed in biomedical field. SiO(2) NPs are primarily designed to enter the circulatory system; however, little information is available on potential adverse effects of SiO(2) NPs on the nervous system. METHODS: The neurotoxicity of SiO(2) NPs at different concentrations (3, 6, 12 ng/nL) on zebrafish embryos was determined using immunofluorescence and microarray techniques, and subsequently confirmed by qRT-PCR. RESULTS: SiO(2) NPs disrupt the axonal integrity and decrease the length of axons in Tg (NBT: EGFP) transgenic lines. The number of apoptotic cells in the brain and central nervous system of zebrafish embryos was increased in the presence of 12 ng/nL of SiO(2) NPs, but the difference did not reach statistical significance. Screening for changes in the expression of genes involved in the neuroactive ligand–receptor interaction pathway was performed by microarray and confirmed by qRT-PCR. These analyses demonstrated that SiO(2) NPs markedly downregulated genes associated with neural function (grm6a, drd1b, chrnb3b, adrb2a, grin2ab, npffr2.1, npy8br, gabrd, chrma3, gabrg3, gria3a, grm1a, adra2b, and glra3). CONCLUSION: The obtained results documented that SiO(2) NPs can induce developmental neurotoxicity by affecting the neuroactive ligand–receptor interaction signaling pathway. This new evidence may help to clarify the mechanism of SiO(2) NPs-mediated neurotoxicity. |
format | Online Article Text |
id | pubmed-7310985 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-73109852020-06-29 Low-Dose Exposure of Silica Nanoparticles Induces Neurotoxicity via Neuroactive Ligand–Receptor Interaction Signaling Pathway in Zebrafish Embryos Wei, Jialiu Liu, Jianhui Liang, Shuang Sun, Mengqi Duan, Junchao Int J Nanomedicine Original Research OBJECTIVE: Silica nanoparticles (SiO(2) NPs) have been extensively employed in biomedical field. SiO(2) NPs are primarily designed to enter the circulatory system; however, little information is available on potential adverse effects of SiO(2) NPs on the nervous system. METHODS: The neurotoxicity of SiO(2) NPs at different concentrations (3, 6, 12 ng/nL) on zebrafish embryos was determined using immunofluorescence and microarray techniques, and subsequently confirmed by qRT-PCR. RESULTS: SiO(2) NPs disrupt the axonal integrity and decrease the length of axons in Tg (NBT: EGFP) transgenic lines. The number of apoptotic cells in the brain and central nervous system of zebrafish embryos was increased in the presence of 12 ng/nL of SiO(2) NPs, but the difference did not reach statistical significance. Screening for changes in the expression of genes involved in the neuroactive ligand–receptor interaction pathway was performed by microarray and confirmed by qRT-PCR. These analyses demonstrated that SiO(2) NPs markedly downregulated genes associated with neural function (grm6a, drd1b, chrnb3b, adrb2a, grin2ab, npffr2.1, npy8br, gabrd, chrma3, gabrg3, gria3a, grm1a, adra2b, and glra3). CONCLUSION: The obtained results documented that SiO(2) NPs can induce developmental neurotoxicity by affecting the neuroactive ligand–receptor interaction signaling pathway. This new evidence may help to clarify the mechanism of SiO(2) NPs-mediated neurotoxicity. Dove 2020-06-19 /pmc/articles/PMC7310985/ /pubmed/32606685 http://dx.doi.org/10.2147/IJN.S254480 Text en © 2020 Wei et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Wei, Jialiu Liu, Jianhui Liang, Shuang Sun, Mengqi Duan, Junchao Low-Dose Exposure of Silica Nanoparticles Induces Neurotoxicity via Neuroactive Ligand–Receptor Interaction Signaling Pathway in Zebrafish Embryos |
title | Low-Dose Exposure of Silica Nanoparticles Induces Neurotoxicity via Neuroactive Ligand–Receptor Interaction Signaling Pathway in Zebrafish Embryos |
title_full | Low-Dose Exposure of Silica Nanoparticles Induces Neurotoxicity via Neuroactive Ligand–Receptor Interaction Signaling Pathway in Zebrafish Embryos |
title_fullStr | Low-Dose Exposure of Silica Nanoparticles Induces Neurotoxicity via Neuroactive Ligand–Receptor Interaction Signaling Pathway in Zebrafish Embryos |
title_full_unstemmed | Low-Dose Exposure of Silica Nanoparticles Induces Neurotoxicity via Neuroactive Ligand–Receptor Interaction Signaling Pathway in Zebrafish Embryos |
title_short | Low-Dose Exposure of Silica Nanoparticles Induces Neurotoxicity via Neuroactive Ligand–Receptor Interaction Signaling Pathway in Zebrafish Embryos |
title_sort | low-dose exposure of silica nanoparticles induces neurotoxicity via neuroactive ligand–receptor interaction signaling pathway in zebrafish embryos |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7310985/ https://www.ncbi.nlm.nih.gov/pubmed/32606685 http://dx.doi.org/10.2147/IJN.S254480 |
work_keys_str_mv | AT weijialiu lowdoseexposureofsilicananoparticlesinducesneurotoxicityvianeuroactiveligandreceptorinteractionsignalingpathwayinzebrafishembryos AT liujianhui lowdoseexposureofsilicananoparticlesinducesneurotoxicityvianeuroactiveligandreceptorinteractionsignalingpathwayinzebrafishembryos AT liangshuang lowdoseexposureofsilicananoparticlesinducesneurotoxicityvianeuroactiveligandreceptorinteractionsignalingpathwayinzebrafishembryos AT sunmengqi lowdoseexposureofsilicananoparticlesinducesneurotoxicityvianeuroactiveligandreceptorinteractionsignalingpathwayinzebrafishembryos AT duanjunchao lowdoseexposureofsilicananoparticlesinducesneurotoxicityvianeuroactiveligandreceptorinteractionsignalingpathwayinzebrafishembryos |