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Genotyping of Fresh and Parafinized Human Hydatid Cysts Using nad1 and cox1 Genes in Hamadan Province, West of Iran

BACKGROUND: Hydatidosis is a cosmopolitan zoonotic infection and Hamadan Province in the west of Iran is one of the most important foci of human hydatidosis in Iran. The aim of the current study was the genetic characterization of hydatid cysts operated from humans in Hamadan Province. METHODS: Seve...

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Autores principales: SHAHBAZI, Ali Ehsan, SAIDIJAM, Massoud, MAGHSOOD, Amir Hossein, MATINI, Mohammad, HAGHI, Moosa MOTAVALI, FALLAH, Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7311807/
https://www.ncbi.nlm.nih.gov/pubmed/32595717
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author SHAHBAZI, Ali Ehsan
SAIDIJAM, Massoud
MAGHSOOD, Amir Hossein
MATINI, Mohammad
HAGHI, Moosa MOTAVALI
FALLAH, Mohammad
author_facet SHAHBAZI, Ali Ehsan
SAIDIJAM, Massoud
MAGHSOOD, Amir Hossein
MATINI, Mohammad
HAGHI, Moosa MOTAVALI
FALLAH, Mohammad
author_sort SHAHBAZI, Ali Ehsan
collection PubMed
description BACKGROUND: Hydatidosis is a cosmopolitan zoonotic infection and Hamadan Province in the west of Iran is one of the most important foci of human hydatidosis in Iran. The aim of the current study was the genetic characterization of hydatid cysts operated from humans in Hamadan Province. METHODS: Seventy-two hydatid cysts samples including 50 paraffinized and 22 fresh human hydatid cysts collected from different hospitals in Hamadan Province, western Iran. The cysts' DNA genome was extracted by kit and PCR was performed for amplifying the fragments of 400 and 450bp for nad1 and cox1 mitochondrial genes, respectively. Genotype diversity and sequence variations of the cysts' isolates were studied by related software. RESULTS: DNA from all (100%) paraffinized and fresh hydatid cysts samples extracted successfully. All paraffinized and fresh hydatid cysts samples were amplified by PCR assay using nad1gene, however, only 18 and 8 samples from paraffinized and fresh hydatid cyst samples was amplified using cox1 gene, respectively. The sequences analysis indicated that, 98.61% the Echinococcus granulosus samples were belong to the genotype G1 and 1.39% were G3 genotype. CONCLUSION: Genotypes of E. granulosus in human samples in Hamadan Province are G1 and G3 and these findings are proved by phylogenic analysis.
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spelling pubmed-73118072020-06-26 Genotyping of Fresh and Parafinized Human Hydatid Cysts Using nad1 and cox1 Genes in Hamadan Province, West of Iran SHAHBAZI, Ali Ehsan SAIDIJAM, Massoud MAGHSOOD, Amir Hossein MATINI, Mohammad HAGHI, Moosa MOTAVALI FALLAH, Mohammad Iran J Parasitol Short Communication BACKGROUND: Hydatidosis is a cosmopolitan zoonotic infection and Hamadan Province in the west of Iran is one of the most important foci of human hydatidosis in Iran. The aim of the current study was the genetic characterization of hydatid cysts operated from humans in Hamadan Province. METHODS: Seventy-two hydatid cysts samples including 50 paraffinized and 22 fresh human hydatid cysts collected from different hospitals in Hamadan Province, western Iran. The cysts' DNA genome was extracted by kit and PCR was performed for amplifying the fragments of 400 and 450bp for nad1 and cox1 mitochondrial genes, respectively. Genotype diversity and sequence variations of the cysts' isolates were studied by related software. RESULTS: DNA from all (100%) paraffinized and fresh hydatid cysts samples extracted successfully. All paraffinized and fresh hydatid cysts samples were amplified by PCR assay using nad1gene, however, only 18 and 8 samples from paraffinized and fresh hydatid cyst samples was amplified using cox1 gene, respectively. The sequences analysis indicated that, 98.61% the Echinococcus granulosus samples were belong to the genotype G1 and 1.39% were G3 genotype. CONCLUSION: Genotypes of E. granulosus in human samples in Hamadan Province are G1 and G3 and these findings are proved by phylogenic analysis. Tehran University of Medical Sciences 2020 /pmc/articles/PMC7311807/ /pubmed/32595717 Text en Copyright© Iranian Society of Parasitology & Tehran University of Medical Sciences http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
SHAHBAZI, Ali Ehsan
SAIDIJAM, Massoud
MAGHSOOD, Amir Hossein
MATINI, Mohammad
HAGHI, Moosa MOTAVALI
FALLAH, Mohammad
Genotyping of Fresh and Parafinized Human Hydatid Cysts Using nad1 and cox1 Genes in Hamadan Province, West of Iran
title Genotyping of Fresh and Parafinized Human Hydatid Cysts Using nad1 and cox1 Genes in Hamadan Province, West of Iran
title_full Genotyping of Fresh and Parafinized Human Hydatid Cysts Using nad1 and cox1 Genes in Hamadan Province, West of Iran
title_fullStr Genotyping of Fresh and Parafinized Human Hydatid Cysts Using nad1 and cox1 Genes in Hamadan Province, West of Iran
title_full_unstemmed Genotyping of Fresh and Parafinized Human Hydatid Cysts Using nad1 and cox1 Genes in Hamadan Province, West of Iran
title_short Genotyping of Fresh and Parafinized Human Hydatid Cysts Using nad1 and cox1 Genes in Hamadan Province, West of Iran
title_sort genotyping of fresh and parafinized human hydatid cysts using nad1 and cox1 genes in hamadan province, west of iran
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7311807/
https://www.ncbi.nlm.nih.gov/pubmed/32595717
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