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Lamprey PHB2 maintains mitochondrial stability by tanslocation to the mitochondria under oxidative stress
Before we have reported lamprey PHB2 could enhance the cellular oxidative-stressed tolerance, here the aim was to explore its mechanisms. We used flow cytometry analysis to identify a Lampetra morii homologue of PHB2 (Lm-PHB2) that could significantly decrease the levels of ROS generation in HEK293T...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Ltd.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7311904/ https://www.ncbi.nlm.nih.gov/pubmed/32592929 http://dx.doi.org/10.1016/j.fsi.2020.06.037 |
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author | Shi, Ying Li, Qing Sun, Feng Zhu, Chenyue Ma, Sainan Qin, Di Li, Qingwei Li, Tiesong |
author_facet | Shi, Ying Li, Qing Sun, Feng Zhu, Chenyue Ma, Sainan Qin, Di Li, Qingwei Li, Tiesong |
author_sort | Shi, Ying |
collection | PubMed |
description | Before we have reported lamprey PHB2 could enhance the cellular oxidative-stressed tolerance, here the aim was to explore its mechanisms. We used flow cytometry analysis to identify a Lampetra morii homologue of PHB2 (Lm-PHB2) that could significantly decrease the levels of ROS generation in HEK293T cells. According to confocal microscopy observations, Lm-PHB2 contributed to maintain the mitochondrial morphology of HEK293T cells, and then both cellular nuclear location and translocation from the nucleus to mitochondria of Lm-PHB2 were also examined in HEK293T cells under oxidative stress. We also examined the expressions and locations of various Lm-PHB2 deletion mutants and the amino acid mutant by confocal microscopy and the results showed that the translocation of Lm-PHB2 into mitochondria was dependent on the Lm-PHB2(1-50aa) region and the 17th, 48th and 57th three arginines (R) of N-terminal were very critical. In addition, the analyses of QRT-PCR and Western blot demonstrated that Lm-PHB2 increased the expression levels of OPA1 and HAX1 in HEK293T cells treated with H(2)O(2). The analyses of immunofluorescence and immunoprecipitation showed that Lm-PHB2 could interact with OPA1 and HAX1, respectively. The above mentioned results indicate that Lm-PHB2 could assist OPA1 and HAX1 to maintain mitochondrial morphology and decrease ROS levels by the translocation from the nucleus to mitochondria under oxidative stress. |
format | Online Article Text |
id | pubmed-7311904 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-73119042020-06-24 Lamprey PHB2 maintains mitochondrial stability by tanslocation to the mitochondria under oxidative stress Shi, Ying Li, Qing Sun, Feng Zhu, Chenyue Ma, Sainan Qin, Di Li, Qingwei Li, Tiesong Fish Shellfish Immunol Article Before we have reported lamprey PHB2 could enhance the cellular oxidative-stressed tolerance, here the aim was to explore its mechanisms. We used flow cytometry analysis to identify a Lampetra morii homologue of PHB2 (Lm-PHB2) that could significantly decrease the levels of ROS generation in HEK293T cells. According to confocal microscopy observations, Lm-PHB2 contributed to maintain the mitochondrial morphology of HEK293T cells, and then both cellular nuclear location and translocation from the nucleus to mitochondria of Lm-PHB2 were also examined in HEK293T cells under oxidative stress. We also examined the expressions and locations of various Lm-PHB2 deletion mutants and the amino acid mutant by confocal microscopy and the results showed that the translocation of Lm-PHB2 into mitochondria was dependent on the Lm-PHB2(1-50aa) region and the 17th, 48th and 57th three arginines (R) of N-terminal were very critical. In addition, the analyses of QRT-PCR and Western blot demonstrated that Lm-PHB2 increased the expression levels of OPA1 and HAX1 in HEK293T cells treated with H(2)O(2). The analyses of immunofluorescence and immunoprecipitation showed that Lm-PHB2 could interact with OPA1 and HAX1, respectively. The above mentioned results indicate that Lm-PHB2 could assist OPA1 and HAX1 to maintain mitochondrial morphology and decrease ROS levels by the translocation from the nucleus to mitochondria under oxidative stress. Elsevier Ltd. 2020-09 2020-06-24 /pmc/articles/PMC7311904/ /pubmed/32592929 http://dx.doi.org/10.1016/j.fsi.2020.06.037 Text en © 2020 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Shi, Ying Li, Qing Sun, Feng Zhu, Chenyue Ma, Sainan Qin, Di Li, Qingwei Li, Tiesong Lamprey PHB2 maintains mitochondrial stability by tanslocation to the mitochondria under oxidative stress |
title | Lamprey PHB2 maintains mitochondrial stability by tanslocation to the mitochondria under oxidative stress |
title_full | Lamprey PHB2 maintains mitochondrial stability by tanslocation to the mitochondria under oxidative stress |
title_fullStr | Lamprey PHB2 maintains mitochondrial stability by tanslocation to the mitochondria under oxidative stress |
title_full_unstemmed | Lamprey PHB2 maintains mitochondrial stability by tanslocation to the mitochondria under oxidative stress |
title_short | Lamprey PHB2 maintains mitochondrial stability by tanslocation to the mitochondria under oxidative stress |
title_sort | lamprey phb2 maintains mitochondrial stability by tanslocation to the mitochondria under oxidative stress |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7311904/ https://www.ncbi.nlm.nih.gov/pubmed/32592929 http://dx.doi.org/10.1016/j.fsi.2020.06.037 |
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