Cargando…

Effective Gold Nanoparticle-Antibody-Mediated Drug Delivery for Photodynamic Therapy of Lung Cancer Stem Cells

Cancer stem cells (CSCs) are a leading contributor to lung cancer mortality rates. CSCs are responsible for tumor growth and recurrence through inhibition of drug-induced cell death, decreasing the effect of traditional cancer therapy and photodynamic therapy (PDT). PDT can be improved to successful...

Descripción completa

Detalles Bibliográficos
Autores principales: Crous, Anine, Abrahamse, Heidi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7311980/
https://www.ncbi.nlm.nih.gov/pubmed/32466428
http://dx.doi.org/10.3390/ijms21113742
_version_ 1783549628465020928
author Crous, Anine
Abrahamse, Heidi
author_facet Crous, Anine
Abrahamse, Heidi
author_sort Crous, Anine
collection PubMed
description Cancer stem cells (CSCs) are a leading contributor to lung cancer mortality rates. CSCs are responsible for tumor growth and recurrence through inhibition of drug-induced cell death, decreasing the effect of traditional cancer therapy and photodynamic therapy (PDT). PDT can be improved to successfully treat lung cancer by using gold nanoparticles (AuNPs), due to their size and shape, which have been shown to facilitate drug delivery and retention, along with the targeted antibody (Ab) mediated selection of CSCs. In this study, a nanobioconjugate (NBC) was constructed, using a photosensitizer (PS) (AlPcS(4)Cl), AuNPs and Abs. The NBC was characterized, using spectroscopy techniques. Photodynamic effects of the NBC on lung CSCs was evaluated, using biochemical assays 24 h post-irradiation, in order to establish its anticancer effect. Results showed successful conjugation of the nanocomposite. Localization of the NBC was seen to be in integral organelles involved in cell homeostasis. Biochemical responses of lung CSCs treated using AlPcS(4)Cl-AuNP and AlPcS(4)Cl-AuNP-Ab showed significant cell toxicity and cell death, compared to free AlPcS(4)Cl. The PDT effects were enhanced when using the NBC, showing significant lung CSC destruction to the point of eradication.
format Online
Article
Text
id pubmed-7311980
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-73119802020-06-25 Effective Gold Nanoparticle-Antibody-Mediated Drug Delivery for Photodynamic Therapy of Lung Cancer Stem Cells Crous, Anine Abrahamse, Heidi Int J Mol Sci Article Cancer stem cells (CSCs) are a leading contributor to lung cancer mortality rates. CSCs are responsible for tumor growth and recurrence through inhibition of drug-induced cell death, decreasing the effect of traditional cancer therapy and photodynamic therapy (PDT). PDT can be improved to successfully treat lung cancer by using gold nanoparticles (AuNPs), due to their size and shape, which have been shown to facilitate drug delivery and retention, along with the targeted antibody (Ab) mediated selection of CSCs. In this study, a nanobioconjugate (NBC) was constructed, using a photosensitizer (PS) (AlPcS(4)Cl), AuNPs and Abs. The NBC was characterized, using spectroscopy techniques. Photodynamic effects of the NBC on lung CSCs was evaluated, using biochemical assays 24 h post-irradiation, in order to establish its anticancer effect. Results showed successful conjugation of the nanocomposite. Localization of the NBC was seen to be in integral organelles involved in cell homeostasis. Biochemical responses of lung CSCs treated using AlPcS(4)Cl-AuNP and AlPcS(4)Cl-AuNP-Ab showed significant cell toxicity and cell death, compared to free AlPcS(4)Cl. The PDT effects were enhanced when using the NBC, showing significant lung CSC destruction to the point of eradication. MDPI 2020-05-26 /pmc/articles/PMC7311980/ /pubmed/32466428 http://dx.doi.org/10.3390/ijms21113742 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Crous, Anine
Abrahamse, Heidi
Effective Gold Nanoparticle-Antibody-Mediated Drug Delivery for Photodynamic Therapy of Lung Cancer Stem Cells
title Effective Gold Nanoparticle-Antibody-Mediated Drug Delivery for Photodynamic Therapy of Lung Cancer Stem Cells
title_full Effective Gold Nanoparticle-Antibody-Mediated Drug Delivery for Photodynamic Therapy of Lung Cancer Stem Cells
title_fullStr Effective Gold Nanoparticle-Antibody-Mediated Drug Delivery for Photodynamic Therapy of Lung Cancer Stem Cells
title_full_unstemmed Effective Gold Nanoparticle-Antibody-Mediated Drug Delivery for Photodynamic Therapy of Lung Cancer Stem Cells
title_short Effective Gold Nanoparticle-Antibody-Mediated Drug Delivery for Photodynamic Therapy of Lung Cancer Stem Cells
title_sort effective gold nanoparticle-antibody-mediated drug delivery for photodynamic therapy of lung cancer stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7311980/
https://www.ncbi.nlm.nih.gov/pubmed/32466428
http://dx.doi.org/10.3390/ijms21113742
work_keys_str_mv AT crousanine effectivegoldnanoparticleantibodymediateddrugdeliveryforphotodynamictherapyoflungcancerstemcells
AT abrahamseheidi effectivegoldnanoparticleantibodymediateddrugdeliveryforphotodynamictherapyoflungcancerstemcells