Cargando…

Skin Mycobiome of Psoriasis Patients is Retained during Treatment with TNF and IL-17 Inhibitors

Background: Biological treatment relieves refractory skin lesions in patients with psoriasis; however, changes in the fungal microbiome (the mycobiome) on the skin are unclear. Methods: The skin mycobiome of psoriasis patients treated with TNF inhibitors (TNFi, n = 5) and IL-17 inhibitors (IL-17i, n...

Descripción completa

Detalles Bibliográficos
Autores principales: Koike, Yuta, Kuwatsuka, Sayaka, Nishimoto, Katsutaro, Motooka, Daisuke, Murota, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312082/
https://www.ncbi.nlm.nih.gov/pubmed/32486022
http://dx.doi.org/10.3390/ijms21113892
_version_ 1783549652606386176
author Koike, Yuta
Kuwatsuka, Sayaka
Nishimoto, Katsutaro
Motooka, Daisuke
Murota, Hiroyuki
author_facet Koike, Yuta
Kuwatsuka, Sayaka
Nishimoto, Katsutaro
Motooka, Daisuke
Murota, Hiroyuki
author_sort Koike, Yuta
collection PubMed
description Background: Biological treatment relieves refractory skin lesions in patients with psoriasis; however, changes in the fungal microbiome (the mycobiome) on the skin are unclear. Methods: The skin mycobiome of psoriasis patients treated with TNF inhibitors (TNFi, n = 5) and IL-17 inhibitors (IL-17i, n = 7) was compared with that of patients not receiving systemic therapy (n = 7). Skin swab samples were collected from non-lesional post-auricular areas. Fungal DNA was sequenced by ITS1 metagenomic analysis and taxonomic classification was performed. Results: An average of 37543 reads/sample were analyzed and fungi belonging to 31 genera were detected. The genus Malassezia accounted for >90% of reads in 7/7 samples from the no-therapy group, 4/5 from the TNFi group, and 5/7 from the IL-17i group. Biodiversity was low in those three groups. Few members of the genus trichophyton were detected; the genus Candida was not detected at all. Among the Malassezia species, M. restricta was the major species in 6/7 samples from the no-therapy group, 4/5 from the TNFi group, and 5/7 from the IL-17i group whose the other largest species revealed M. globosa. Conclusions: The mycobiome is retained on post-auricular skin during systemic treatment with TNF and IL-17 inhibitors.
format Online
Article
Text
id pubmed-7312082
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-73120822020-06-25 Skin Mycobiome of Psoriasis Patients is Retained during Treatment with TNF and IL-17 Inhibitors Koike, Yuta Kuwatsuka, Sayaka Nishimoto, Katsutaro Motooka, Daisuke Murota, Hiroyuki Int J Mol Sci Article Background: Biological treatment relieves refractory skin lesions in patients with psoriasis; however, changes in the fungal microbiome (the mycobiome) on the skin are unclear. Methods: The skin mycobiome of psoriasis patients treated with TNF inhibitors (TNFi, n = 5) and IL-17 inhibitors (IL-17i, n = 7) was compared with that of patients not receiving systemic therapy (n = 7). Skin swab samples were collected from non-lesional post-auricular areas. Fungal DNA was sequenced by ITS1 metagenomic analysis and taxonomic classification was performed. Results: An average of 37543 reads/sample were analyzed and fungi belonging to 31 genera were detected. The genus Malassezia accounted for >90% of reads in 7/7 samples from the no-therapy group, 4/5 from the TNFi group, and 5/7 from the IL-17i group. Biodiversity was low in those three groups. Few members of the genus trichophyton were detected; the genus Candida was not detected at all. Among the Malassezia species, M. restricta was the major species in 6/7 samples from the no-therapy group, 4/5 from the TNFi group, and 5/7 from the IL-17i group whose the other largest species revealed M. globosa. Conclusions: The mycobiome is retained on post-auricular skin during systemic treatment with TNF and IL-17 inhibitors. MDPI 2020-05-29 /pmc/articles/PMC7312082/ /pubmed/32486022 http://dx.doi.org/10.3390/ijms21113892 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Koike, Yuta
Kuwatsuka, Sayaka
Nishimoto, Katsutaro
Motooka, Daisuke
Murota, Hiroyuki
Skin Mycobiome of Psoriasis Patients is Retained during Treatment with TNF and IL-17 Inhibitors
title Skin Mycobiome of Psoriasis Patients is Retained during Treatment with TNF and IL-17 Inhibitors
title_full Skin Mycobiome of Psoriasis Patients is Retained during Treatment with TNF and IL-17 Inhibitors
title_fullStr Skin Mycobiome of Psoriasis Patients is Retained during Treatment with TNF and IL-17 Inhibitors
title_full_unstemmed Skin Mycobiome of Psoriasis Patients is Retained during Treatment with TNF and IL-17 Inhibitors
title_short Skin Mycobiome of Psoriasis Patients is Retained during Treatment with TNF and IL-17 Inhibitors
title_sort skin mycobiome of psoriasis patients is retained during treatment with tnf and il-17 inhibitors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312082/
https://www.ncbi.nlm.nih.gov/pubmed/32486022
http://dx.doi.org/10.3390/ijms21113892
work_keys_str_mv AT koikeyuta skinmycobiomeofpsoriasispatientsisretainedduringtreatmentwithtnfandil17inhibitors
AT kuwatsukasayaka skinmycobiomeofpsoriasispatientsisretainedduringtreatmentwithtnfandil17inhibitors
AT nishimotokatsutaro skinmycobiomeofpsoriasispatientsisretainedduringtreatmentwithtnfandil17inhibitors
AT motookadaisuke skinmycobiomeofpsoriasispatientsisretainedduringtreatmentwithtnfandil17inhibitors
AT murotahiroyuki skinmycobiomeofpsoriasispatientsisretainedduringtreatmentwithtnfandil17inhibitors