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Aptamer Cocktail to Detect Multiple Species of Mycoplasma in Cell Culture

Mycoplasma contamination of cell line cultures is a common, yet often undetected problem in research laboratories. Many of the existing techniques to detect mycoplasma contamination of cultured cells are time-consuming, expensive, and have significant drawbacks. Here, we describe a mycoplasma detect...

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Autores principales: Wan, Quanyuan, Liu, Xiaohui, Zeng, Zihua, Chen, Zhenghu, Liu, Yanting, Zu, Youli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312096/
https://www.ncbi.nlm.nih.gov/pubmed/32471128
http://dx.doi.org/10.3390/ijms21113784
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author Wan, Quanyuan
Liu, Xiaohui
Zeng, Zihua
Chen, Zhenghu
Liu, Yanting
Zu, Youli
author_facet Wan, Quanyuan
Liu, Xiaohui
Zeng, Zihua
Chen, Zhenghu
Liu, Yanting
Zu, Youli
author_sort Wan, Quanyuan
collection PubMed
description Mycoplasma contamination of cell line cultures is a common, yet often undetected problem in research laboratories. Many of the existing techniques to detect mycoplasma contamination of cultured cells are time-consuming, expensive, and have significant drawbacks. Here, we describe a mycoplasma detection system that is useful for detecting multiple species of mycoplasma in infected cell lines. The system contains three dye-labeled detection aptamers that can specifically bind to mycoplasma-infected cells and a dye-labeled control aptamer that minimally binds to cells. With this system, mycoplasma-contaminated cells can be detected within 30 min by using a flow cytometer, fluorescence microscope, or microplate reader. Further, this system may be used to detect mycoplasma-contaminated culture medium. This study presents an novel mycoplasma detection model that is simple, rapid, inexpensive, and sensitive.
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spelling pubmed-73120962020-06-25 Aptamer Cocktail to Detect Multiple Species of Mycoplasma in Cell Culture Wan, Quanyuan Liu, Xiaohui Zeng, Zihua Chen, Zhenghu Liu, Yanting Zu, Youli Int J Mol Sci Article Mycoplasma contamination of cell line cultures is a common, yet often undetected problem in research laboratories. Many of the existing techniques to detect mycoplasma contamination of cultured cells are time-consuming, expensive, and have significant drawbacks. Here, we describe a mycoplasma detection system that is useful for detecting multiple species of mycoplasma in infected cell lines. The system contains three dye-labeled detection aptamers that can specifically bind to mycoplasma-infected cells and a dye-labeled control aptamer that minimally binds to cells. With this system, mycoplasma-contaminated cells can be detected within 30 min by using a flow cytometer, fluorescence microscope, or microplate reader. Further, this system may be used to detect mycoplasma-contaminated culture medium. This study presents an novel mycoplasma detection model that is simple, rapid, inexpensive, and sensitive. MDPI 2020-05-27 /pmc/articles/PMC7312096/ /pubmed/32471128 http://dx.doi.org/10.3390/ijms21113784 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wan, Quanyuan
Liu, Xiaohui
Zeng, Zihua
Chen, Zhenghu
Liu, Yanting
Zu, Youli
Aptamer Cocktail to Detect Multiple Species of Mycoplasma in Cell Culture
title Aptamer Cocktail to Detect Multiple Species of Mycoplasma in Cell Culture
title_full Aptamer Cocktail to Detect Multiple Species of Mycoplasma in Cell Culture
title_fullStr Aptamer Cocktail to Detect Multiple Species of Mycoplasma in Cell Culture
title_full_unstemmed Aptamer Cocktail to Detect Multiple Species of Mycoplasma in Cell Culture
title_short Aptamer Cocktail to Detect Multiple Species of Mycoplasma in Cell Culture
title_sort aptamer cocktail to detect multiple species of mycoplasma in cell culture
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312096/
https://www.ncbi.nlm.nih.gov/pubmed/32471128
http://dx.doi.org/10.3390/ijms21113784
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