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Aptamer Cocktail to Detect Multiple Species of Mycoplasma in Cell Culture
Mycoplasma contamination of cell line cultures is a common, yet often undetected problem in research laboratories. Many of the existing techniques to detect mycoplasma contamination of cultured cells are time-consuming, expensive, and have significant drawbacks. Here, we describe a mycoplasma detect...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312096/ https://www.ncbi.nlm.nih.gov/pubmed/32471128 http://dx.doi.org/10.3390/ijms21113784 |
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author | Wan, Quanyuan Liu, Xiaohui Zeng, Zihua Chen, Zhenghu Liu, Yanting Zu, Youli |
author_facet | Wan, Quanyuan Liu, Xiaohui Zeng, Zihua Chen, Zhenghu Liu, Yanting Zu, Youli |
author_sort | Wan, Quanyuan |
collection | PubMed |
description | Mycoplasma contamination of cell line cultures is a common, yet often undetected problem in research laboratories. Many of the existing techniques to detect mycoplasma contamination of cultured cells are time-consuming, expensive, and have significant drawbacks. Here, we describe a mycoplasma detection system that is useful for detecting multiple species of mycoplasma in infected cell lines. The system contains three dye-labeled detection aptamers that can specifically bind to mycoplasma-infected cells and a dye-labeled control aptamer that minimally binds to cells. With this system, mycoplasma-contaminated cells can be detected within 30 min by using a flow cytometer, fluorescence microscope, or microplate reader. Further, this system may be used to detect mycoplasma-contaminated culture medium. This study presents an novel mycoplasma detection model that is simple, rapid, inexpensive, and sensitive. |
format | Online Article Text |
id | pubmed-7312096 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73120962020-06-25 Aptamer Cocktail to Detect Multiple Species of Mycoplasma in Cell Culture Wan, Quanyuan Liu, Xiaohui Zeng, Zihua Chen, Zhenghu Liu, Yanting Zu, Youli Int J Mol Sci Article Mycoplasma contamination of cell line cultures is a common, yet often undetected problem in research laboratories. Many of the existing techniques to detect mycoplasma contamination of cultured cells are time-consuming, expensive, and have significant drawbacks. Here, we describe a mycoplasma detection system that is useful for detecting multiple species of mycoplasma in infected cell lines. The system contains three dye-labeled detection aptamers that can specifically bind to mycoplasma-infected cells and a dye-labeled control aptamer that minimally binds to cells. With this system, mycoplasma-contaminated cells can be detected within 30 min by using a flow cytometer, fluorescence microscope, or microplate reader. Further, this system may be used to detect mycoplasma-contaminated culture medium. This study presents an novel mycoplasma detection model that is simple, rapid, inexpensive, and sensitive. MDPI 2020-05-27 /pmc/articles/PMC7312096/ /pubmed/32471128 http://dx.doi.org/10.3390/ijms21113784 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wan, Quanyuan Liu, Xiaohui Zeng, Zihua Chen, Zhenghu Liu, Yanting Zu, Youli Aptamer Cocktail to Detect Multiple Species of Mycoplasma in Cell Culture |
title | Aptamer Cocktail to Detect Multiple Species of Mycoplasma in Cell Culture |
title_full | Aptamer Cocktail to Detect Multiple Species of Mycoplasma in Cell Culture |
title_fullStr | Aptamer Cocktail to Detect Multiple Species of Mycoplasma in Cell Culture |
title_full_unstemmed | Aptamer Cocktail to Detect Multiple Species of Mycoplasma in Cell Culture |
title_short | Aptamer Cocktail to Detect Multiple Species of Mycoplasma in Cell Culture |
title_sort | aptamer cocktail to detect multiple species of mycoplasma in cell culture |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312096/ https://www.ncbi.nlm.nih.gov/pubmed/32471128 http://dx.doi.org/10.3390/ijms21113784 |
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