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Optimization of crystallization of biological macromolecules using dialysis combined with temperature control
A rational way to find the appropriate conditions to grow crystal samples for bio-crystallography is to determine the crystallization phase diagram, which allows precise control of the parameters affecting the crystal growth process. First, the nucleation is induced at supersaturated conditions clos...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312135/ https://www.ncbi.nlm.nih.gov/pubmed/32684884 http://dx.doi.org/10.1107/S1600576720003209 |
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author | Junius, Niels Vahdatahar, Elham Oksanen, Esko Ferrer, Jean-Luc Budayova-Spano, Monika |
author_facet | Junius, Niels Vahdatahar, Elham Oksanen, Esko Ferrer, Jean-Luc Budayova-Spano, Monika |
author_sort | Junius, Niels |
collection | PubMed |
description | A rational way to find the appropriate conditions to grow crystal samples for bio-crystallography is to determine the crystallization phase diagram, which allows precise control of the parameters affecting the crystal growth process. First, the nucleation is induced at supersaturated conditions close to the solubility boundary between the nucleation and metastable regions. Then, crystal growth is further achieved in the metastable zone – which is the optimal location for slow and ordered crystal expansion – by modulation of specific physical parameters. Recently, a prototype of an integrated apparatus for the rational optimization of crystal growth by mapping and manipulating temperature–precipitant–concentration phase diagrams has been constructed. Here, it is demonstrated that a thorough knowledge of the phase diagram is vital in any crystallization experiment. The relevance of the selection of the starting position and the kinetic pathway undertaken in controlling most of the final properties of the synthesized crystals is shown. The rational crystallization optimization strategies developed and presented here allow tailoring of crystal size and diffraction quality, significantly reducing the time, effort and amount of expensive protein material required for structure determination. |
format | Online Article Text |
id | pubmed-7312135 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-73121352020-07-16 Optimization of crystallization of biological macromolecules using dialysis combined with temperature control Junius, Niels Vahdatahar, Elham Oksanen, Esko Ferrer, Jean-Luc Budayova-Spano, Monika J Appl Crystallogr Research Papers A rational way to find the appropriate conditions to grow crystal samples for bio-crystallography is to determine the crystallization phase diagram, which allows precise control of the parameters affecting the crystal growth process. First, the nucleation is induced at supersaturated conditions close to the solubility boundary between the nucleation and metastable regions. Then, crystal growth is further achieved in the metastable zone – which is the optimal location for slow and ordered crystal expansion – by modulation of specific physical parameters. Recently, a prototype of an integrated apparatus for the rational optimization of crystal growth by mapping and manipulating temperature–precipitant–concentration phase diagrams has been constructed. Here, it is demonstrated that a thorough knowledge of the phase diagram is vital in any crystallization experiment. The relevance of the selection of the starting position and the kinetic pathway undertaken in controlling most of the final properties of the synthesized crystals is shown. The rational crystallization optimization strategies developed and presented here allow tailoring of crystal size and diffraction quality, significantly reducing the time, effort and amount of expensive protein material required for structure determination. International Union of Crystallography 2020-05-05 /pmc/articles/PMC7312135/ /pubmed/32684884 http://dx.doi.org/10.1107/S1600576720003209 Text en © Niels Junius et al. 2020 http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Research Papers Junius, Niels Vahdatahar, Elham Oksanen, Esko Ferrer, Jean-Luc Budayova-Spano, Monika Optimization of crystallization of biological macromolecules using dialysis combined with temperature control |
title | Optimization of crystallization of biological macromolecules using dialysis combined with temperature control |
title_full | Optimization of crystallization of biological macromolecules using dialysis combined with temperature control |
title_fullStr | Optimization of crystallization of biological macromolecules using dialysis combined with temperature control |
title_full_unstemmed | Optimization of crystallization of biological macromolecules using dialysis combined with temperature control |
title_short | Optimization of crystallization of biological macromolecules using dialysis combined with temperature control |
title_sort | optimization of crystallization of biological macromolecules using dialysis combined with temperature control |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312135/ https://www.ncbi.nlm.nih.gov/pubmed/32684884 http://dx.doi.org/10.1107/S1600576720003209 |
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