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The Effect of Sodium Bicarbonate, a Beneficial Adjuvant Molecule in Cystic Fibrosis, on Bronchial Epithelial Cells Expressing a Wild-Type or Mutant CFTR Channel

Clinical and experimental results with inhaled sodium bicarbonate as an adjuvant therapy in cystic fibrosis (CF) are promising due to its mucolytic and bacteriostatic properties, but its direct effect has not been studied on respiratory epithelial cells. Our aim was to establish and characterize co-...

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Autores principales: Gróf, Ilona, Bocsik, Alexandra, Harazin, András, Santa-Maria, Ana Raquel, Vizsnyiczai, Gaszton, Barna, Lilla, Kiss, Lóránd, Fűr, Gabriella, Rakonczay, Zoltán, Ambrus, Rita, Szabó-Révész, Piroska, Gosselet, Fabien, Jaikumpun, Pongsiri, Szabó, Hajnalka, Zsembery, Ákos, Deli, Mária A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312297/
https://www.ncbi.nlm.nih.gov/pubmed/32512832
http://dx.doi.org/10.3390/ijms21114024
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author Gróf, Ilona
Bocsik, Alexandra
Harazin, András
Santa-Maria, Ana Raquel
Vizsnyiczai, Gaszton
Barna, Lilla
Kiss, Lóránd
Fűr, Gabriella
Rakonczay, Zoltán
Ambrus, Rita
Szabó-Révész, Piroska
Gosselet, Fabien
Jaikumpun, Pongsiri
Szabó, Hajnalka
Zsembery, Ákos
Deli, Mária A.
author_facet Gróf, Ilona
Bocsik, Alexandra
Harazin, András
Santa-Maria, Ana Raquel
Vizsnyiczai, Gaszton
Barna, Lilla
Kiss, Lóránd
Fűr, Gabriella
Rakonczay, Zoltán
Ambrus, Rita
Szabó-Révész, Piroska
Gosselet, Fabien
Jaikumpun, Pongsiri
Szabó, Hajnalka
Zsembery, Ákos
Deli, Mária A.
author_sort Gróf, Ilona
collection PubMed
description Clinical and experimental results with inhaled sodium bicarbonate as an adjuvant therapy in cystic fibrosis (CF) are promising due to its mucolytic and bacteriostatic properties, but its direct effect has not been studied on respiratory epithelial cells. Our aim was to establish and characterize co-culture models of human CF bronchial epithelial (CFBE) cell lines expressing a wild-type (WT) or mutant (deltaF508) CF transmembrane conductance regulator (CFTR) channel with human vascular endothelial cells and investigate the effects of bicarbonate. Vascular endothelial cells induced better barrier properties in CFBE cells as reflected by the higher resistance and lower permeability values. Activation of CFTR by cAMP decreased the electrical resistance in WT but not in mutant CFBE cell layers confirming the presence and absence of functional channels, respectively. Sodium bicarbonate (100 mM) was well-tolerated by CFBE cells: it slightly reduced the impedance of WT but not that of the mutant CFBE cells. Sodium bicarbonate significantly decreased the more-alkaline intracellular pH of the mutant CFBE cells, while the barrier properties of the models were only minimally changed. These observations indicate that sodium bicarbonate is beneficial to deltaF508-CFTR expressing CFBE cells. Thus, sodium bicarbonate may have a direct therapeutic effect on the bronchial epithelium.
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spelling pubmed-73122972020-06-26 The Effect of Sodium Bicarbonate, a Beneficial Adjuvant Molecule in Cystic Fibrosis, on Bronchial Epithelial Cells Expressing a Wild-Type or Mutant CFTR Channel Gróf, Ilona Bocsik, Alexandra Harazin, András Santa-Maria, Ana Raquel Vizsnyiczai, Gaszton Barna, Lilla Kiss, Lóránd Fűr, Gabriella Rakonczay, Zoltán Ambrus, Rita Szabó-Révész, Piroska Gosselet, Fabien Jaikumpun, Pongsiri Szabó, Hajnalka Zsembery, Ákos Deli, Mária A. Int J Mol Sci Article Clinical and experimental results with inhaled sodium bicarbonate as an adjuvant therapy in cystic fibrosis (CF) are promising due to its mucolytic and bacteriostatic properties, but its direct effect has not been studied on respiratory epithelial cells. Our aim was to establish and characterize co-culture models of human CF bronchial epithelial (CFBE) cell lines expressing a wild-type (WT) or mutant (deltaF508) CF transmembrane conductance regulator (CFTR) channel with human vascular endothelial cells and investigate the effects of bicarbonate. Vascular endothelial cells induced better barrier properties in CFBE cells as reflected by the higher resistance and lower permeability values. Activation of CFTR by cAMP decreased the electrical resistance in WT but not in mutant CFBE cell layers confirming the presence and absence of functional channels, respectively. Sodium bicarbonate (100 mM) was well-tolerated by CFBE cells: it slightly reduced the impedance of WT but not that of the mutant CFBE cells. Sodium bicarbonate significantly decreased the more-alkaline intracellular pH of the mutant CFBE cells, while the barrier properties of the models were only minimally changed. These observations indicate that sodium bicarbonate is beneficial to deltaF508-CFTR expressing CFBE cells. Thus, sodium bicarbonate may have a direct therapeutic effect on the bronchial epithelium. MDPI 2020-06-04 /pmc/articles/PMC7312297/ /pubmed/32512832 http://dx.doi.org/10.3390/ijms21114024 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gróf, Ilona
Bocsik, Alexandra
Harazin, András
Santa-Maria, Ana Raquel
Vizsnyiczai, Gaszton
Barna, Lilla
Kiss, Lóránd
Fűr, Gabriella
Rakonczay, Zoltán
Ambrus, Rita
Szabó-Révész, Piroska
Gosselet, Fabien
Jaikumpun, Pongsiri
Szabó, Hajnalka
Zsembery, Ákos
Deli, Mária A.
The Effect of Sodium Bicarbonate, a Beneficial Adjuvant Molecule in Cystic Fibrosis, on Bronchial Epithelial Cells Expressing a Wild-Type or Mutant CFTR Channel
title The Effect of Sodium Bicarbonate, a Beneficial Adjuvant Molecule in Cystic Fibrosis, on Bronchial Epithelial Cells Expressing a Wild-Type or Mutant CFTR Channel
title_full The Effect of Sodium Bicarbonate, a Beneficial Adjuvant Molecule in Cystic Fibrosis, on Bronchial Epithelial Cells Expressing a Wild-Type or Mutant CFTR Channel
title_fullStr The Effect of Sodium Bicarbonate, a Beneficial Adjuvant Molecule in Cystic Fibrosis, on Bronchial Epithelial Cells Expressing a Wild-Type or Mutant CFTR Channel
title_full_unstemmed The Effect of Sodium Bicarbonate, a Beneficial Adjuvant Molecule in Cystic Fibrosis, on Bronchial Epithelial Cells Expressing a Wild-Type or Mutant CFTR Channel
title_short The Effect of Sodium Bicarbonate, a Beneficial Adjuvant Molecule in Cystic Fibrosis, on Bronchial Epithelial Cells Expressing a Wild-Type or Mutant CFTR Channel
title_sort effect of sodium bicarbonate, a beneficial adjuvant molecule in cystic fibrosis, on bronchial epithelial cells expressing a wild-type or mutant cftr channel
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312297/
https://www.ncbi.nlm.nih.gov/pubmed/32512832
http://dx.doi.org/10.3390/ijms21114024
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