Cargando…
Relationship between IL-8 Circulating Levels and TLR2 Hepatic Expression in Women with Morbid Obesity and Nonalcoholic Steatohepatitis
The progression of nonalcoholic fatty liver disease (NAFLD) to nonalcoholic steatohepatitis (NASH) is linked to systemic inflammation. Currently, two of the aspects that need further investigation are diagnosis and treatment of NASH. In this sense, the aim of this study was to assess the relationshi...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312372/ https://www.ncbi.nlm.nih.gov/pubmed/32545403 http://dx.doi.org/10.3390/ijms21114189 |
_version_ | 1783549712998072320 |
---|---|
author | Auguet, Teresa Bertran, Laia Binetti, Jessica Aguilar, Carmen Martínez, Salomé Sabench, Fàtima Lopez-Dupla, Jesús Miguel Porras, José Antonio Riesco, David Del Castillo, Daniel Richart, Cristóbal |
author_facet | Auguet, Teresa Bertran, Laia Binetti, Jessica Aguilar, Carmen Martínez, Salomé Sabench, Fàtima Lopez-Dupla, Jesús Miguel Porras, José Antonio Riesco, David Del Castillo, Daniel Richart, Cristóbal |
author_sort | Auguet, Teresa |
collection | PubMed |
description | The progression of nonalcoholic fatty liver disease (NAFLD) to nonalcoholic steatohepatitis (NASH) is linked to systemic inflammation. Currently, two of the aspects that need further investigation are diagnosis and treatment of NASH. In this sense, the aim of this study was to assess the relationship between circulating levels of cytokines, hepatic expression of toll-like receptors (TLRs), and degrees of NAFLD, and to investigate whether these levels could serve as noninvasive biomarkers of NASH. The present study assessed plasma levels of cytokines in 29 normal-weight women and 82 women with morbid obesity (MO) (subclassified: normal liver (n = 29), simple steatosis (n = 32), and NASH (n = 21)). We used enzyme-linked immunosorbent assays (ELISAs) to quantify cytokine and TLR4 levels and RTqPCR to assess TLRs hepatic expression. IL-1β, IL-8, IL-10, TNF-α, tPAI-1, and MCP-1 levels were increased, and adiponectin levels were decreased in women with MO. IL-8 was significantly higher in MO with NASH than in NL. To sum up, high levels of IL-8 were associated with the diagnosis of NASH in a cohort of women with morbid obesity. Moreover, a positive correlation between TLR2 hepatic expression and IL-8 circulating levels was found. |
format | Online Article Text |
id | pubmed-7312372 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73123722020-06-26 Relationship between IL-8 Circulating Levels and TLR2 Hepatic Expression in Women with Morbid Obesity and Nonalcoholic Steatohepatitis Auguet, Teresa Bertran, Laia Binetti, Jessica Aguilar, Carmen Martínez, Salomé Sabench, Fàtima Lopez-Dupla, Jesús Miguel Porras, José Antonio Riesco, David Del Castillo, Daniel Richart, Cristóbal Int J Mol Sci Article The progression of nonalcoholic fatty liver disease (NAFLD) to nonalcoholic steatohepatitis (NASH) is linked to systemic inflammation. Currently, two of the aspects that need further investigation are diagnosis and treatment of NASH. In this sense, the aim of this study was to assess the relationship between circulating levels of cytokines, hepatic expression of toll-like receptors (TLRs), and degrees of NAFLD, and to investigate whether these levels could serve as noninvasive biomarkers of NASH. The present study assessed plasma levels of cytokines in 29 normal-weight women and 82 women with morbid obesity (MO) (subclassified: normal liver (n = 29), simple steatosis (n = 32), and NASH (n = 21)). We used enzyme-linked immunosorbent assays (ELISAs) to quantify cytokine and TLR4 levels and RTqPCR to assess TLRs hepatic expression. IL-1β, IL-8, IL-10, TNF-α, tPAI-1, and MCP-1 levels were increased, and adiponectin levels were decreased in women with MO. IL-8 was significantly higher in MO with NASH than in NL. To sum up, high levels of IL-8 were associated with the diagnosis of NASH in a cohort of women with morbid obesity. Moreover, a positive correlation between TLR2 hepatic expression and IL-8 circulating levels was found. MDPI 2020-06-11 /pmc/articles/PMC7312372/ /pubmed/32545403 http://dx.doi.org/10.3390/ijms21114189 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Auguet, Teresa Bertran, Laia Binetti, Jessica Aguilar, Carmen Martínez, Salomé Sabench, Fàtima Lopez-Dupla, Jesús Miguel Porras, José Antonio Riesco, David Del Castillo, Daniel Richart, Cristóbal Relationship between IL-8 Circulating Levels and TLR2 Hepatic Expression in Women with Morbid Obesity and Nonalcoholic Steatohepatitis |
title | Relationship between IL-8 Circulating Levels and TLR2 Hepatic Expression in Women with Morbid Obesity and Nonalcoholic Steatohepatitis |
title_full | Relationship between IL-8 Circulating Levels and TLR2 Hepatic Expression in Women with Morbid Obesity and Nonalcoholic Steatohepatitis |
title_fullStr | Relationship between IL-8 Circulating Levels and TLR2 Hepatic Expression in Women with Morbid Obesity and Nonalcoholic Steatohepatitis |
title_full_unstemmed | Relationship between IL-8 Circulating Levels and TLR2 Hepatic Expression in Women with Morbid Obesity and Nonalcoholic Steatohepatitis |
title_short | Relationship between IL-8 Circulating Levels and TLR2 Hepatic Expression in Women with Morbid Obesity and Nonalcoholic Steatohepatitis |
title_sort | relationship between il-8 circulating levels and tlr2 hepatic expression in women with morbid obesity and nonalcoholic steatohepatitis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312372/ https://www.ncbi.nlm.nih.gov/pubmed/32545403 http://dx.doi.org/10.3390/ijms21114189 |
work_keys_str_mv | AT auguetteresa relationshipbetweenil8circulatinglevelsandtlr2hepaticexpressioninwomenwithmorbidobesityandnonalcoholicsteatohepatitis AT bertranlaia relationshipbetweenil8circulatinglevelsandtlr2hepaticexpressioninwomenwithmorbidobesityandnonalcoholicsteatohepatitis AT binettijessica relationshipbetweenil8circulatinglevelsandtlr2hepaticexpressioninwomenwithmorbidobesityandnonalcoholicsteatohepatitis AT aguilarcarmen relationshipbetweenil8circulatinglevelsandtlr2hepaticexpressioninwomenwithmorbidobesityandnonalcoholicsteatohepatitis AT martinezsalome relationshipbetweenil8circulatinglevelsandtlr2hepaticexpressioninwomenwithmorbidobesityandnonalcoholicsteatohepatitis AT sabenchfatima relationshipbetweenil8circulatinglevelsandtlr2hepaticexpressioninwomenwithmorbidobesityandnonalcoholicsteatohepatitis AT lopezduplajesusmiguel relationshipbetweenil8circulatinglevelsandtlr2hepaticexpressioninwomenwithmorbidobesityandnonalcoholicsteatohepatitis AT porrasjoseantonio relationshipbetweenil8circulatinglevelsandtlr2hepaticexpressioninwomenwithmorbidobesityandnonalcoholicsteatohepatitis AT riescodavid relationshipbetweenil8circulatinglevelsandtlr2hepaticexpressioninwomenwithmorbidobesityandnonalcoholicsteatohepatitis AT delcastillodaniel relationshipbetweenil8circulatinglevelsandtlr2hepaticexpressioninwomenwithmorbidobesityandnonalcoholicsteatohepatitis AT richartcristobal relationshipbetweenil8circulatinglevelsandtlr2hepaticexpressioninwomenwithmorbidobesityandnonalcoholicsteatohepatitis |