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Oxytocin Controls Chondrogenesis and Correlates with Osteoarthritis

This study investigated the relationship of oxytocin (OT) to chondrogenesis and osteoarthritis (OA). Human bone marrow and multipotent adipose-derived stem cells were cultured in vitro in the absence or presence of OT and assayed for mRNA transcript expression along with histological and immunohisto...

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Autores principales: Roux, Christian H., Pisani, Didier F., Gillet, Pierre, Fontas, Eric, Yahia, Hédi Ben, Djedaini, Mansour, Ambrosetti, Damien, Michiels, Jean-François, Panaia-Ferrari, Patricia, Breuil, Véronique, Pinzano, Astrid, Amri, Ez-Zoubir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312425/
https://www.ncbi.nlm.nih.gov/pubmed/32486506
http://dx.doi.org/10.3390/ijms21113966
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author Roux, Christian H.
Pisani, Didier F.
Gillet, Pierre
Fontas, Eric
Yahia, Hédi Ben
Djedaini, Mansour
Ambrosetti, Damien
Michiels, Jean-François
Panaia-Ferrari, Patricia
Breuil, Véronique
Pinzano, Astrid
Amri, Ez-Zoubir
author_facet Roux, Christian H.
Pisani, Didier F.
Gillet, Pierre
Fontas, Eric
Yahia, Hédi Ben
Djedaini, Mansour
Ambrosetti, Damien
Michiels, Jean-François
Panaia-Ferrari, Patricia
Breuil, Véronique
Pinzano, Astrid
Amri, Ez-Zoubir
author_sort Roux, Christian H.
collection PubMed
description This study investigated the relationship of oxytocin (OT) to chondrogenesis and osteoarthritis (OA). Human bone marrow and multipotent adipose-derived stem cells were cultured in vitro in the absence or presence of OT and assayed for mRNA transcript expression along with histological and immunohistochemical analyses. To study the effects of OT in OA in vivo, a rat model and a human cohort of 63 men and 19 women with hand OA and healthy controls, respectively, were used. The baseline circulating OT, interleukin-6, leptin, and oestradiol levels were measured, and hand X-ray examinations were performed for each subject. OT induced increased aggrecan, collagen (Col) X, and cartilage oligomeric matrix protein mRNA transcript levels in vitro, and the immunolabelling experiments revealed a normalization of Sox9 and Col II protein expression levels. No histological differences in lesion severity were observed between rat OA groups. In the clinical study, a multivariate analysis adjusted for age, body mass index, and leptin levels revealed a significant association between OA and lower levels of OT (odds ratio = 0.77; p = 0.012). Serum OT levels are reduced in patients with hand OA, and OT showed a stimulatory effect on chondrogenesis. Thus, OT may contribute to the pathophysiology of OA.
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spelling pubmed-73124252020-06-26 Oxytocin Controls Chondrogenesis and Correlates with Osteoarthritis Roux, Christian H. Pisani, Didier F. Gillet, Pierre Fontas, Eric Yahia, Hédi Ben Djedaini, Mansour Ambrosetti, Damien Michiels, Jean-François Panaia-Ferrari, Patricia Breuil, Véronique Pinzano, Astrid Amri, Ez-Zoubir Int J Mol Sci Article This study investigated the relationship of oxytocin (OT) to chondrogenesis and osteoarthritis (OA). Human bone marrow and multipotent adipose-derived stem cells were cultured in vitro in the absence or presence of OT and assayed for mRNA transcript expression along with histological and immunohistochemical analyses. To study the effects of OT in OA in vivo, a rat model and a human cohort of 63 men and 19 women with hand OA and healthy controls, respectively, were used. The baseline circulating OT, interleukin-6, leptin, and oestradiol levels were measured, and hand X-ray examinations were performed for each subject. OT induced increased aggrecan, collagen (Col) X, and cartilage oligomeric matrix protein mRNA transcript levels in vitro, and the immunolabelling experiments revealed a normalization of Sox9 and Col II protein expression levels. No histological differences in lesion severity were observed between rat OA groups. In the clinical study, a multivariate analysis adjusted for age, body mass index, and leptin levels revealed a significant association between OA and lower levels of OT (odds ratio = 0.77; p = 0.012). Serum OT levels are reduced in patients with hand OA, and OT showed a stimulatory effect on chondrogenesis. Thus, OT may contribute to the pathophysiology of OA. MDPI 2020-05-31 /pmc/articles/PMC7312425/ /pubmed/32486506 http://dx.doi.org/10.3390/ijms21113966 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Roux, Christian H.
Pisani, Didier F.
Gillet, Pierre
Fontas, Eric
Yahia, Hédi Ben
Djedaini, Mansour
Ambrosetti, Damien
Michiels, Jean-François
Panaia-Ferrari, Patricia
Breuil, Véronique
Pinzano, Astrid
Amri, Ez-Zoubir
Oxytocin Controls Chondrogenesis and Correlates with Osteoarthritis
title Oxytocin Controls Chondrogenesis and Correlates with Osteoarthritis
title_full Oxytocin Controls Chondrogenesis and Correlates with Osteoarthritis
title_fullStr Oxytocin Controls Chondrogenesis and Correlates with Osteoarthritis
title_full_unstemmed Oxytocin Controls Chondrogenesis and Correlates with Osteoarthritis
title_short Oxytocin Controls Chondrogenesis and Correlates with Osteoarthritis
title_sort oxytocin controls chondrogenesis and correlates with osteoarthritis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312425/
https://www.ncbi.nlm.nih.gov/pubmed/32486506
http://dx.doi.org/10.3390/ijms21113966
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