Cargando…
Intensification of Ex Situ Bioremediation of Soils Polluted with Used Lubricant Oils: A Comparison of Biostimulation and Bioaugmentation with a Special Focus on the Type and Size of the Inoculum
Used lubricant oils (ULOs) strongly bind to soil particles and cause persistent pollution. In this study, soil microcosm experiments were conducted to model the ex situ bioremediation of a long term ULO-polluted area. Biostimulation and various inoculation levels of bioaugmentation were applied to d...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312492/ https://www.ncbi.nlm.nih.gov/pubmed/32526873 http://dx.doi.org/10.3390/ijerph17114106 |
_version_ | 1783549740393168896 |
---|---|
author | Bodor, Attila Petrovszki, Péter Erdeiné Kis, Ágnes Vincze, György Erik Laczi, Krisztián Bounedjoum, Naila Szilágyi, Árpád Szalontai, Balázs Feigl, Gábor Kovács, Kornél L. Rákhely, Gábor Perei, Katalin |
author_facet | Bodor, Attila Petrovszki, Péter Erdeiné Kis, Ágnes Vincze, György Erik Laczi, Krisztián Bounedjoum, Naila Szilágyi, Árpád Szalontai, Balázs Feigl, Gábor Kovács, Kornél L. Rákhely, Gábor Perei, Katalin |
author_sort | Bodor, Attila |
collection | PubMed |
description | Used lubricant oils (ULOs) strongly bind to soil particles and cause persistent pollution. In this study, soil microcosm experiments were conducted to model the ex situ bioremediation of a long term ULO-polluted area. Biostimulation and various inoculation levels of bioaugmentation were applied to determine the efficacy of total petrol hydrocarbon (TPH) removal. ULO-contaminated soil microcosms were monitored for microbial respiration, colony-forming units (CFUs) and TPH bioconversion. Biostimulation with inorganic nutrients was responsible for 22% of ULO removal after 40 days. Bioaugmentation using two hydrocarbon-degrader strains: Rhodococcus quingshengii KAG C and Rhodococcus erythropolis PR4 at a small inoculum size (10(7) CFUs g(−1) soil), reduced initial TPH concentration by 24% and 29%, respectively; the application of a higher inoculum size (10(9) CFUs g(−1) soil) led to 41% and 32% bioconversion, respectively. After 20 days, all augmented CFUs decreased to the same level as measured in the biostimulated cases, substantiating the challenge for the newly introduced hydrocarbon-degrading strains to cope with environmental stressors. Our results not only highlight that an increased number of degrader cells does not always correlate with enhanced TPH bioconversion, but they also indicate that biostimulation might be an economical solution to promote ULO biodegradation in long term contaminated soils. |
format | Online Article Text |
id | pubmed-7312492 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73124922020-06-29 Intensification of Ex Situ Bioremediation of Soils Polluted with Used Lubricant Oils: A Comparison of Biostimulation and Bioaugmentation with a Special Focus on the Type and Size of the Inoculum Bodor, Attila Petrovszki, Péter Erdeiné Kis, Ágnes Vincze, György Erik Laczi, Krisztián Bounedjoum, Naila Szilágyi, Árpád Szalontai, Balázs Feigl, Gábor Kovács, Kornél L. Rákhely, Gábor Perei, Katalin Int J Environ Res Public Health Article Used lubricant oils (ULOs) strongly bind to soil particles and cause persistent pollution. In this study, soil microcosm experiments were conducted to model the ex situ bioremediation of a long term ULO-polluted area. Biostimulation and various inoculation levels of bioaugmentation were applied to determine the efficacy of total petrol hydrocarbon (TPH) removal. ULO-contaminated soil microcosms were monitored for microbial respiration, colony-forming units (CFUs) and TPH bioconversion. Biostimulation with inorganic nutrients was responsible for 22% of ULO removal after 40 days. Bioaugmentation using two hydrocarbon-degrader strains: Rhodococcus quingshengii KAG C and Rhodococcus erythropolis PR4 at a small inoculum size (10(7) CFUs g(−1) soil), reduced initial TPH concentration by 24% and 29%, respectively; the application of a higher inoculum size (10(9) CFUs g(−1) soil) led to 41% and 32% bioconversion, respectively. After 20 days, all augmented CFUs decreased to the same level as measured in the biostimulated cases, substantiating the challenge for the newly introduced hydrocarbon-degrading strains to cope with environmental stressors. Our results not only highlight that an increased number of degrader cells does not always correlate with enhanced TPH bioconversion, but they also indicate that biostimulation might be an economical solution to promote ULO biodegradation in long term contaminated soils. MDPI 2020-06-09 2020-06 /pmc/articles/PMC7312492/ /pubmed/32526873 http://dx.doi.org/10.3390/ijerph17114106 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bodor, Attila Petrovszki, Péter Erdeiné Kis, Ágnes Vincze, György Erik Laczi, Krisztián Bounedjoum, Naila Szilágyi, Árpád Szalontai, Balázs Feigl, Gábor Kovács, Kornél L. Rákhely, Gábor Perei, Katalin Intensification of Ex Situ Bioremediation of Soils Polluted with Used Lubricant Oils: A Comparison of Biostimulation and Bioaugmentation with a Special Focus on the Type and Size of the Inoculum |
title | Intensification of Ex Situ Bioremediation of Soils Polluted with Used Lubricant Oils: A Comparison of Biostimulation and Bioaugmentation with a Special Focus on the Type and Size of the Inoculum |
title_full | Intensification of Ex Situ Bioremediation of Soils Polluted with Used Lubricant Oils: A Comparison of Biostimulation and Bioaugmentation with a Special Focus on the Type and Size of the Inoculum |
title_fullStr | Intensification of Ex Situ Bioremediation of Soils Polluted with Used Lubricant Oils: A Comparison of Biostimulation and Bioaugmentation with a Special Focus on the Type and Size of the Inoculum |
title_full_unstemmed | Intensification of Ex Situ Bioremediation of Soils Polluted with Used Lubricant Oils: A Comparison of Biostimulation and Bioaugmentation with a Special Focus on the Type and Size of the Inoculum |
title_short | Intensification of Ex Situ Bioremediation of Soils Polluted with Used Lubricant Oils: A Comparison of Biostimulation and Bioaugmentation with a Special Focus on the Type and Size of the Inoculum |
title_sort | intensification of ex situ bioremediation of soils polluted with used lubricant oils: a comparison of biostimulation and bioaugmentation with a special focus on the type and size of the inoculum |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312492/ https://www.ncbi.nlm.nih.gov/pubmed/32526873 http://dx.doi.org/10.3390/ijerph17114106 |
work_keys_str_mv | AT bodorattila intensificationofexsitubioremediationofsoilspollutedwithusedlubricantoilsacomparisonofbiostimulationandbioaugmentationwithaspecialfocusonthetypeandsizeoftheinoculum AT petrovszkipeter intensificationofexsitubioremediationofsoilspollutedwithusedlubricantoilsacomparisonofbiostimulationandbioaugmentationwithaspecialfocusonthetypeandsizeoftheinoculum AT erdeinekisagnes intensificationofexsitubioremediationofsoilspollutedwithusedlubricantoilsacomparisonofbiostimulationandbioaugmentationwithaspecialfocusonthetypeandsizeoftheinoculum AT vinczegyorgyerik intensificationofexsitubioremediationofsoilspollutedwithusedlubricantoilsacomparisonofbiostimulationandbioaugmentationwithaspecialfocusonthetypeandsizeoftheinoculum AT laczikrisztian intensificationofexsitubioremediationofsoilspollutedwithusedlubricantoilsacomparisonofbiostimulationandbioaugmentationwithaspecialfocusonthetypeandsizeoftheinoculum AT bounedjoumnaila intensificationofexsitubioremediationofsoilspollutedwithusedlubricantoilsacomparisonofbiostimulationandbioaugmentationwithaspecialfocusonthetypeandsizeoftheinoculum AT szilagyiarpad intensificationofexsitubioremediationofsoilspollutedwithusedlubricantoilsacomparisonofbiostimulationandbioaugmentationwithaspecialfocusonthetypeandsizeoftheinoculum AT szalontaibalazs intensificationofexsitubioremediationofsoilspollutedwithusedlubricantoilsacomparisonofbiostimulationandbioaugmentationwithaspecialfocusonthetypeandsizeoftheinoculum AT feiglgabor intensificationofexsitubioremediationofsoilspollutedwithusedlubricantoilsacomparisonofbiostimulationandbioaugmentationwithaspecialfocusonthetypeandsizeoftheinoculum AT kovacskornell intensificationofexsitubioremediationofsoilspollutedwithusedlubricantoilsacomparisonofbiostimulationandbioaugmentationwithaspecialfocusonthetypeandsizeoftheinoculum AT rakhelygabor intensificationofexsitubioremediationofsoilspollutedwithusedlubricantoilsacomparisonofbiostimulationandbioaugmentationwithaspecialfocusonthetypeandsizeoftheinoculum AT pereikatalin intensificationofexsitubioremediationofsoilspollutedwithusedlubricantoilsacomparisonofbiostimulationandbioaugmentationwithaspecialfocusonthetypeandsizeoftheinoculum |