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Intensification of Ex Situ Bioremediation of Soils Polluted with Used Lubricant Oils: A Comparison of Biostimulation and Bioaugmentation with a Special Focus on the Type and Size of the Inoculum

Used lubricant oils (ULOs) strongly bind to soil particles and cause persistent pollution. In this study, soil microcosm experiments were conducted to model the ex situ bioremediation of a long term ULO-polluted area. Biostimulation and various inoculation levels of bioaugmentation were applied to d...

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Autores principales: Bodor, Attila, Petrovszki, Péter, Erdeiné Kis, Ágnes, Vincze, György Erik, Laczi, Krisztián, Bounedjoum, Naila, Szilágyi, Árpád, Szalontai, Balázs, Feigl, Gábor, Kovács, Kornél L., Rákhely, Gábor, Perei, Katalin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312492/
https://www.ncbi.nlm.nih.gov/pubmed/32526873
http://dx.doi.org/10.3390/ijerph17114106
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author Bodor, Attila
Petrovszki, Péter
Erdeiné Kis, Ágnes
Vincze, György Erik
Laczi, Krisztián
Bounedjoum, Naila
Szilágyi, Árpád
Szalontai, Balázs
Feigl, Gábor
Kovács, Kornél L.
Rákhely, Gábor
Perei, Katalin
author_facet Bodor, Attila
Petrovszki, Péter
Erdeiné Kis, Ágnes
Vincze, György Erik
Laczi, Krisztián
Bounedjoum, Naila
Szilágyi, Árpád
Szalontai, Balázs
Feigl, Gábor
Kovács, Kornél L.
Rákhely, Gábor
Perei, Katalin
author_sort Bodor, Attila
collection PubMed
description Used lubricant oils (ULOs) strongly bind to soil particles and cause persistent pollution. In this study, soil microcosm experiments were conducted to model the ex situ bioremediation of a long term ULO-polluted area. Biostimulation and various inoculation levels of bioaugmentation were applied to determine the efficacy of total petrol hydrocarbon (TPH) removal. ULO-contaminated soil microcosms were monitored for microbial respiration, colony-forming units (CFUs) and TPH bioconversion. Biostimulation with inorganic nutrients was responsible for 22% of ULO removal after 40 days. Bioaugmentation using two hydrocarbon-degrader strains: Rhodococcus quingshengii KAG C and Rhodococcus erythropolis PR4 at a small inoculum size (10(7) CFUs g(−1) soil), reduced initial TPH concentration by 24% and 29%, respectively; the application of a higher inoculum size (10(9) CFUs g(−1) soil) led to 41% and 32% bioconversion, respectively. After 20 days, all augmented CFUs decreased to the same level as measured in the biostimulated cases, substantiating the challenge for the newly introduced hydrocarbon-degrading strains to cope with environmental stressors. Our results not only highlight that an increased number of degrader cells does not always correlate with enhanced TPH bioconversion, but they also indicate that biostimulation might be an economical solution to promote ULO biodegradation in long term contaminated soils.
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spelling pubmed-73124922020-06-29 Intensification of Ex Situ Bioremediation of Soils Polluted with Used Lubricant Oils: A Comparison of Biostimulation and Bioaugmentation with a Special Focus on the Type and Size of the Inoculum Bodor, Attila Petrovszki, Péter Erdeiné Kis, Ágnes Vincze, György Erik Laczi, Krisztián Bounedjoum, Naila Szilágyi, Árpád Szalontai, Balázs Feigl, Gábor Kovács, Kornél L. Rákhely, Gábor Perei, Katalin Int J Environ Res Public Health Article Used lubricant oils (ULOs) strongly bind to soil particles and cause persistent pollution. In this study, soil microcosm experiments were conducted to model the ex situ bioremediation of a long term ULO-polluted area. Biostimulation and various inoculation levels of bioaugmentation were applied to determine the efficacy of total petrol hydrocarbon (TPH) removal. ULO-contaminated soil microcosms were monitored for microbial respiration, colony-forming units (CFUs) and TPH bioconversion. Biostimulation with inorganic nutrients was responsible for 22% of ULO removal after 40 days. Bioaugmentation using two hydrocarbon-degrader strains: Rhodococcus quingshengii KAG C and Rhodococcus erythropolis PR4 at a small inoculum size (10(7) CFUs g(−1) soil), reduced initial TPH concentration by 24% and 29%, respectively; the application of a higher inoculum size (10(9) CFUs g(−1) soil) led to 41% and 32% bioconversion, respectively. After 20 days, all augmented CFUs decreased to the same level as measured in the biostimulated cases, substantiating the challenge for the newly introduced hydrocarbon-degrading strains to cope with environmental stressors. Our results not only highlight that an increased number of degrader cells does not always correlate with enhanced TPH bioconversion, but they also indicate that biostimulation might be an economical solution to promote ULO biodegradation in long term contaminated soils. MDPI 2020-06-09 2020-06 /pmc/articles/PMC7312492/ /pubmed/32526873 http://dx.doi.org/10.3390/ijerph17114106 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bodor, Attila
Petrovszki, Péter
Erdeiné Kis, Ágnes
Vincze, György Erik
Laczi, Krisztián
Bounedjoum, Naila
Szilágyi, Árpád
Szalontai, Balázs
Feigl, Gábor
Kovács, Kornél L.
Rákhely, Gábor
Perei, Katalin
Intensification of Ex Situ Bioremediation of Soils Polluted with Used Lubricant Oils: A Comparison of Biostimulation and Bioaugmentation with a Special Focus on the Type and Size of the Inoculum
title Intensification of Ex Situ Bioremediation of Soils Polluted with Used Lubricant Oils: A Comparison of Biostimulation and Bioaugmentation with a Special Focus on the Type and Size of the Inoculum
title_full Intensification of Ex Situ Bioremediation of Soils Polluted with Used Lubricant Oils: A Comparison of Biostimulation and Bioaugmentation with a Special Focus on the Type and Size of the Inoculum
title_fullStr Intensification of Ex Situ Bioremediation of Soils Polluted with Used Lubricant Oils: A Comparison of Biostimulation and Bioaugmentation with a Special Focus on the Type and Size of the Inoculum
title_full_unstemmed Intensification of Ex Situ Bioremediation of Soils Polluted with Used Lubricant Oils: A Comparison of Biostimulation and Bioaugmentation with a Special Focus on the Type and Size of the Inoculum
title_short Intensification of Ex Situ Bioremediation of Soils Polluted with Used Lubricant Oils: A Comparison of Biostimulation and Bioaugmentation with a Special Focus on the Type and Size of the Inoculum
title_sort intensification of ex situ bioremediation of soils polluted with used lubricant oils: a comparison of biostimulation and bioaugmentation with a special focus on the type and size of the inoculum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312492/
https://www.ncbi.nlm.nih.gov/pubmed/32526873
http://dx.doi.org/10.3390/ijerph17114106
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