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DI-5-Cuffs: Lumbar Intervertebral Disc Proteoglycan and Water Content Changes in Humans after Five Days of Dry Immersion to Simulate Microgravity

Most astronauts experience back pain after spaceflight, primarily located in the lumbar region. Intervertebral disc herniations have been observed after real and simulated microgravity. Spinal deconditioning after exposure to microgravity has been described, but the underlying mechanisms are not wel...

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Autores principales: Loïc, Treffel, Nastassia, Navasiolava, Mkhitaryan, Karen, Emmanuelle, Jouan, Kathryn, Zuj, Guillemette, Gauquelin-Koch, Marc-Antoine, Custaud, Claude, Gharib
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312650/
https://www.ncbi.nlm.nih.gov/pubmed/32466473
http://dx.doi.org/10.3390/ijms21113748
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author Loïc, Treffel
Nastassia, Navasiolava
Mkhitaryan, Karen
Emmanuelle, Jouan
Kathryn, Zuj
Guillemette, Gauquelin-Koch
Marc-Antoine, Custaud
Claude, Gharib
author_facet Loïc, Treffel
Nastassia, Navasiolava
Mkhitaryan, Karen
Emmanuelle, Jouan
Kathryn, Zuj
Guillemette, Gauquelin-Koch
Marc-Antoine, Custaud
Claude, Gharib
author_sort Loïc, Treffel
collection PubMed
description Most astronauts experience back pain after spaceflight, primarily located in the lumbar region. Intervertebral disc herniations have been observed after real and simulated microgravity. Spinal deconditioning after exposure to microgravity has been described, but the underlying mechanisms are not well understood. The dry immersion (DI) model of microgravity was used with eighteen male volunteers. Half of the participants wore thigh cuffs as a potential countermeasure. The spinal changes and intervertebral disc (IVD) content changes were investigated using magnetic resonance imaging (MRI) analyses with T1-T2 mapping sequences. IVD water content was estimated by the apparent diffusion coefficient (ADC), with proteoglycan content measured using MRI T1-mapping sequences centered in the nucleus pulposus. The use of thigh cuffs had no effect on any of the spinal variables measured. There was significant spinal lengthening for all of the subjects. The ADC and IVD proteoglycan content both increased significantly with DI (7.34 ± 2.23% and 10.09 ± 1.39%, respectively; mean ± standard deviation), p < 0.05). The ADC changes suggest dynamic and rapid water diffusion inside IVDs, linked to gravitational unloading. Further investigation is needed to determine whether similar changes occur in the cervical IVDs. A better understanding of the mechanisms involved in spinal deconditioning with spaceflight would assist in the development of alternative countermeasures to prevent IVD herniation.
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spelling pubmed-73126502020-06-26 DI-5-Cuffs: Lumbar Intervertebral Disc Proteoglycan and Water Content Changes in Humans after Five Days of Dry Immersion to Simulate Microgravity Loïc, Treffel Nastassia, Navasiolava Mkhitaryan, Karen Emmanuelle, Jouan Kathryn, Zuj Guillemette, Gauquelin-Koch Marc-Antoine, Custaud Claude, Gharib Int J Mol Sci Article Most astronauts experience back pain after spaceflight, primarily located in the lumbar region. Intervertebral disc herniations have been observed after real and simulated microgravity. Spinal deconditioning after exposure to microgravity has been described, but the underlying mechanisms are not well understood. The dry immersion (DI) model of microgravity was used with eighteen male volunteers. Half of the participants wore thigh cuffs as a potential countermeasure. The spinal changes and intervertebral disc (IVD) content changes were investigated using magnetic resonance imaging (MRI) analyses with T1-T2 mapping sequences. IVD water content was estimated by the apparent diffusion coefficient (ADC), with proteoglycan content measured using MRI T1-mapping sequences centered in the nucleus pulposus. The use of thigh cuffs had no effect on any of the spinal variables measured. There was significant spinal lengthening for all of the subjects. The ADC and IVD proteoglycan content both increased significantly with DI (7.34 ± 2.23% and 10.09 ± 1.39%, respectively; mean ± standard deviation), p < 0.05). The ADC changes suggest dynamic and rapid water diffusion inside IVDs, linked to gravitational unloading. Further investigation is needed to determine whether similar changes occur in the cervical IVDs. A better understanding of the mechanisms involved in spinal deconditioning with spaceflight would assist in the development of alternative countermeasures to prevent IVD herniation. MDPI 2020-05-26 /pmc/articles/PMC7312650/ /pubmed/32466473 http://dx.doi.org/10.3390/ijms21113748 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Loïc, Treffel
Nastassia, Navasiolava
Mkhitaryan, Karen
Emmanuelle, Jouan
Kathryn, Zuj
Guillemette, Gauquelin-Koch
Marc-Antoine, Custaud
Claude, Gharib
DI-5-Cuffs: Lumbar Intervertebral Disc Proteoglycan and Water Content Changes in Humans after Five Days of Dry Immersion to Simulate Microgravity
title DI-5-Cuffs: Lumbar Intervertebral Disc Proteoglycan and Water Content Changes in Humans after Five Days of Dry Immersion to Simulate Microgravity
title_full DI-5-Cuffs: Lumbar Intervertebral Disc Proteoglycan and Water Content Changes in Humans after Five Days of Dry Immersion to Simulate Microgravity
title_fullStr DI-5-Cuffs: Lumbar Intervertebral Disc Proteoglycan and Water Content Changes in Humans after Five Days of Dry Immersion to Simulate Microgravity
title_full_unstemmed DI-5-Cuffs: Lumbar Intervertebral Disc Proteoglycan and Water Content Changes in Humans after Five Days of Dry Immersion to Simulate Microgravity
title_short DI-5-Cuffs: Lumbar Intervertebral Disc Proteoglycan and Water Content Changes in Humans after Five Days of Dry Immersion to Simulate Microgravity
title_sort di-5-cuffs: lumbar intervertebral disc proteoglycan and water content changes in humans after five days of dry immersion to simulate microgravity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312650/
https://www.ncbi.nlm.nih.gov/pubmed/32466473
http://dx.doi.org/10.3390/ijms21113748
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