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Oral Microbiome of Children Living in an Isolated Area in Myanmar

Several studies have shown that the oral microbiome is related to systemic health, and a co-relation with several specific diseases has been suggested. The oral microbiome depends on environmental- and community-level factors. In this observational study, the oral microbiomes of children of isolated...

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Autores principales: Nomura, Yoshiaki, Otsuka, Ryoko, Hasegawa, Ryo, Hanada, Nobuhiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312721/
https://www.ncbi.nlm.nih.gov/pubmed/32517039
http://dx.doi.org/10.3390/ijerph17114033
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author Nomura, Yoshiaki
Otsuka, Ryoko
Hasegawa, Ryo
Hanada, Nobuhiro
author_facet Nomura, Yoshiaki
Otsuka, Ryoko
Hasegawa, Ryo
Hanada, Nobuhiro
author_sort Nomura, Yoshiaki
collection PubMed
description Several studies have shown that the oral microbiome is related to systemic health, and a co-relation with several specific diseases has been suggested. The oral microbiome depends on environmental- and community-level factors. In this observational study, the oral microbiomes of children of isolated mountain people were analyzed with respect to the core oral microbiome and etiology of dental caries. We collected samples of supragingival plaque from children (age 9–13) living in the Chin state of Myanmar. After DNA extraction and purification, next-generation sequencing of the V3–V4 hypervariable regions of the 16S rRNA was conducted. From thirteen subjects, 263,458 valid reads and 640 operational taxonomic units were generated at a 97% identity cut-off value. At the phylum level, Proteobacteria was the most abundant, followed by Firmicutes and Bacteroides. Forty-four bacteria were detected in total from all the subjects. For children without dental caries, Proteobacteria was abundant. In contrast, in children with dental caries, Firmicutes and Bacteroides were abundant. The oral microbiome of children living in an isolated area may be affected by environmental- and community-level factors. Additionally, the composition of the oral microbiome may affect the risk of dental caries.
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spelling pubmed-73127212020-06-26 Oral Microbiome of Children Living in an Isolated Area in Myanmar Nomura, Yoshiaki Otsuka, Ryoko Hasegawa, Ryo Hanada, Nobuhiro Int J Environ Res Public Health Article Several studies have shown that the oral microbiome is related to systemic health, and a co-relation with several specific diseases has been suggested. The oral microbiome depends on environmental- and community-level factors. In this observational study, the oral microbiomes of children of isolated mountain people were analyzed with respect to the core oral microbiome and etiology of dental caries. We collected samples of supragingival plaque from children (age 9–13) living in the Chin state of Myanmar. After DNA extraction and purification, next-generation sequencing of the V3–V4 hypervariable regions of the 16S rRNA was conducted. From thirteen subjects, 263,458 valid reads and 640 operational taxonomic units were generated at a 97% identity cut-off value. At the phylum level, Proteobacteria was the most abundant, followed by Firmicutes and Bacteroides. Forty-four bacteria were detected in total from all the subjects. For children without dental caries, Proteobacteria was abundant. In contrast, in children with dental caries, Firmicutes and Bacteroides were abundant. The oral microbiome of children living in an isolated area may be affected by environmental- and community-level factors. Additionally, the composition of the oral microbiome may affect the risk of dental caries. MDPI 2020-06-05 2020-06 /pmc/articles/PMC7312721/ /pubmed/32517039 http://dx.doi.org/10.3390/ijerph17114033 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nomura, Yoshiaki
Otsuka, Ryoko
Hasegawa, Ryo
Hanada, Nobuhiro
Oral Microbiome of Children Living in an Isolated Area in Myanmar
title Oral Microbiome of Children Living in an Isolated Area in Myanmar
title_full Oral Microbiome of Children Living in an Isolated Area in Myanmar
title_fullStr Oral Microbiome of Children Living in an Isolated Area in Myanmar
title_full_unstemmed Oral Microbiome of Children Living in an Isolated Area in Myanmar
title_short Oral Microbiome of Children Living in an Isolated Area in Myanmar
title_sort oral microbiome of children living in an isolated area in myanmar
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312721/
https://www.ncbi.nlm.nih.gov/pubmed/32517039
http://dx.doi.org/10.3390/ijerph17114033
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