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Oral Microbiome of Children Living in an Isolated Area in Myanmar
Several studies have shown that the oral microbiome is related to systemic health, and a co-relation with several specific diseases has been suggested. The oral microbiome depends on environmental- and community-level factors. In this observational study, the oral microbiomes of children of isolated...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312721/ https://www.ncbi.nlm.nih.gov/pubmed/32517039 http://dx.doi.org/10.3390/ijerph17114033 |
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author | Nomura, Yoshiaki Otsuka, Ryoko Hasegawa, Ryo Hanada, Nobuhiro |
author_facet | Nomura, Yoshiaki Otsuka, Ryoko Hasegawa, Ryo Hanada, Nobuhiro |
author_sort | Nomura, Yoshiaki |
collection | PubMed |
description | Several studies have shown that the oral microbiome is related to systemic health, and a co-relation with several specific diseases has been suggested. The oral microbiome depends on environmental- and community-level factors. In this observational study, the oral microbiomes of children of isolated mountain people were analyzed with respect to the core oral microbiome and etiology of dental caries. We collected samples of supragingival plaque from children (age 9–13) living in the Chin state of Myanmar. After DNA extraction and purification, next-generation sequencing of the V3–V4 hypervariable regions of the 16S rRNA was conducted. From thirteen subjects, 263,458 valid reads and 640 operational taxonomic units were generated at a 97% identity cut-off value. At the phylum level, Proteobacteria was the most abundant, followed by Firmicutes and Bacteroides. Forty-four bacteria were detected in total from all the subjects. For children without dental caries, Proteobacteria was abundant. In contrast, in children with dental caries, Firmicutes and Bacteroides were abundant. The oral microbiome of children living in an isolated area may be affected by environmental- and community-level factors. Additionally, the composition of the oral microbiome may affect the risk of dental caries. |
format | Online Article Text |
id | pubmed-7312721 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73127212020-06-26 Oral Microbiome of Children Living in an Isolated Area in Myanmar Nomura, Yoshiaki Otsuka, Ryoko Hasegawa, Ryo Hanada, Nobuhiro Int J Environ Res Public Health Article Several studies have shown that the oral microbiome is related to systemic health, and a co-relation with several specific diseases has been suggested. The oral microbiome depends on environmental- and community-level factors. In this observational study, the oral microbiomes of children of isolated mountain people were analyzed with respect to the core oral microbiome and etiology of dental caries. We collected samples of supragingival plaque from children (age 9–13) living in the Chin state of Myanmar. After DNA extraction and purification, next-generation sequencing of the V3–V4 hypervariable regions of the 16S rRNA was conducted. From thirteen subjects, 263,458 valid reads and 640 operational taxonomic units were generated at a 97% identity cut-off value. At the phylum level, Proteobacteria was the most abundant, followed by Firmicutes and Bacteroides. Forty-four bacteria were detected in total from all the subjects. For children without dental caries, Proteobacteria was abundant. In contrast, in children with dental caries, Firmicutes and Bacteroides were abundant. The oral microbiome of children living in an isolated area may be affected by environmental- and community-level factors. Additionally, the composition of the oral microbiome may affect the risk of dental caries. MDPI 2020-06-05 2020-06 /pmc/articles/PMC7312721/ /pubmed/32517039 http://dx.doi.org/10.3390/ijerph17114033 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nomura, Yoshiaki Otsuka, Ryoko Hasegawa, Ryo Hanada, Nobuhiro Oral Microbiome of Children Living in an Isolated Area in Myanmar |
title | Oral Microbiome of Children Living in an Isolated Area in Myanmar |
title_full | Oral Microbiome of Children Living in an Isolated Area in Myanmar |
title_fullStr | Oral Microbiome of Children Living in an Isolated Area in Myanmar |
title_full_unstemmed | Oral Microbiome of Children Living in an Isolated Area in Myanmar |
title_short | Oral Microbiome of Children Living in an Isolated Area in Myanmar |
title_sort | oral microbiome of children living in an isolated area in myanmar |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312721/ https://www.ncbi.nlm.nih.gov/pubmed/32517039 http://dx.doi.org/10.3390/ijerph17114033 |
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